Plasmid DNA Purification using QIAprep Spin Miniprep Kit and Vacuum Manifold (Updated Version)

The classic QIAGEN bind-wash-wash-elute protocol for the QIAprep Spin Miniprep Kit involves several centrifugation steps. While guaranteeing excellent flow-through of all the wash buffers and eluates, loading spin columns into the centrifuge also requires extra handling steps. But surprisingly, you can save up to 10 minutes by doing the wash steps on a vacuum device.

[@EddieBoyle-l7m]

Davis Joseph Davis Larry Thompson Brenda

[@issaynarumi4088]

Wash using 0.5 ml Buffer PB, and then wash using 0.75 ml Buffer PE. But, the volume of the former seems the same as that of latter.

[@Anonymous9683]

I realize this was 5 years ago and is entirely inconsequential, but I can't help myself. Of course the easy answer is that they reused the footage since why would you not, but how do you know for sure that's what they did? The only answer I've been able to come up with is flicking quickly between frames of the 0.5 ml and 0.75 ml footage and noting that the positions of the two frontmost valves are seemingly identical between the two shots. I think the chances of that happening are really slim. Anyways, back to work

[@jebl0001]

Do you have a product number for the vacuum manifold?