Collect the DNA and use PCR to amplify it. Restriction endonucleases are used to hydrolyse the DNA. Use gel electrophoresis to separate the DNA fragments. The DNA fragments are separated based on charge with smaller fragments of DNA moving faster and further. The DNA should be treated with an alkali to make it single stranded. The DNA probes can then be added and would hybridise with the DNA fragments. Wash away excess probes which did not bind. The probe would have fluorescence or a radioactive tracer bound to it. Use UV light or an audioradiography to determine if the gene/allele/VNTR is present.
Ok, so after the separation of DNA at the separation stage of electrophoresis, the DNA probes are just added to the gel? Where on the gel? How are the probes added? How do they randonly just mix with the single stranded DNA VNTRS?
Hi miss, I was just wondering how would this be used for medical diagnosis as I thought that VNTR’s are part of introns, so surely they don’t do anything because they don’t code for proteins so how could they cause disease?
I think it's because the VNTRs don't cause the disease, but certain VNTRs are common in people with the disease and so if present can indicate the disease. But I may be wrong! In my textbook it says 'Genetic fingerprinting can help in diagnosing diseases such as Huntington's disease. It results from AGC on one end of chromosome 4 being repeated over and over again.'
There are 2 methods to amplify DNA, in vivo and in vitro(PCR). Why is it that we can only use PCR and not in vivo? Is it because in vivo deals with bacteria cells or?
On the child's fingerprint the 6th band from the top doesn't have an equivalent band on the mother nor any of the father's fingerprints. How does this work? Also, why are some of the mother's bands not present in the child's fingerprint? Thanks
Hello, I have had a look and I think this is an error in that image. Not all of the mother's bands will be present in the child's fingerprint as the child's DNA is a mix of the mother and father's DNA not a complete copy of either parent. I hope that helps.
Hello, I have had a look and I think this is an error in that image. Not all of the mother's bands will be present in the child's fingerprint as the child's DNA is a mix of the mother and father's DNA not a complete copy of either parent. I hope that helps.
![I.N "HALLUCINATION" | [Stray Kids : SKZ-PLAYER]](http://i.ytimg.com/vi/n5B5q1Hwt_U/mqdefault.jpg)
Thank you so much , Praise to Allah that I found this video, saved my A Levels Bio
Thank you ☺ That's so great to hear how much the videos are helping you!
Best of luck with your A-levels 🤞
What grade did you get mate?
Collect the DNA and use PCR to amplify it.
Restriction endonucleases are used to hydrolyse the DNA.
Use gel electrophoresis to separate the DNA fragments.
The DNA fragments are separated based on charge with smaller fragments of DNA moving faster and further.
The DNA should be treated with an alkali to make it single stranded.
The DNA probes can then be added and would hybridise with the DNA fragments.
Wash away excess probes which did not bind.
The probe would have fluorescence or a radioactive tracer bound to it.
Use UV light or an audioradiography to determine if the gene/allele/VNTR is present.
THANK YOU
🤓🤓🤓
@@qasimkhan34😂
So brilliant..i'm teaching this topic at A level for the first time and its so easy to understand now! 🙏
😁😁 so happy to have helped!
Uses:
Forensic Science
Paternity Testing
Medical Diagnosis
Animal and Plant Breeding Programmes
your videos are so helpfull! thank you!
ahh so pleased ☺ Hope they continue to help you with your studies.
11:47 why dose band 6 from top of child not overlap with the mother or father
Ok, so after the separation of DNA at the separation stage of electrophoresis, the DNA probes are just added to the gel? Where on the gel? How are the probes added? How do they randonly just mix with the single stranded DNA VNTRS?
Hi miss, I was just wondering how would this be used for medical diagnosis as I thought that VNTR’s are part of introns, so surely they don’t do anything because they don’t code for proteins so how could they cause disease?
I think it's because the VNTRs don't cause the disease, but certain VNTRs are common in people with the disease and so if present can indicate the disease. But I may be wrong! In my textbook it says 'Genetic fingerprinting can help in diagnosing diseases such as Huntington's disease. It results from AGC on one end of chromosome 4 being repeated over and over again.'
Really helpful for my EUT thank you!
You're very welcome!
Your grades were E U and T???!
@@miscellaneousz2681 I believe it’s end of unit test
There are 2 methods to amplify DNA, in vivo and in vitro(PCR). Why is it that we can only use PCR and not in vivo? Is it because in vivo deals with bacteria cells or?
In vivo is used sometimes. PCR is automated and rapid so easier
Thank you for this video!
You are so welcome!
On the child's fingerprint the 6th band from the top doesn't have an equivalent band on the mother nor any of the father's fingerprints. How does this work? Also, why are some of the mother's bands not present in the child's fingerprint? Thanks
Hello,
I have had a look and I think this is an error in that image.
Not all of the mother's bands will be present in the child's fingerprint as the child's DNA is a mix of the mother and father's DNA not a complete copy of either parent.
I hope that helps.
@@MissEstruchBiology could it the top band have occurred due to mutations
@@MissEstruchBiology Could it have been due to a mutation in the Childs DNA/genes?
very informative!
Thank you 😊 Hope it helped you
11:42 the 4th yellow band for the child doesn’t match father 1 or any other father so where has that come from?
Hello,
I have had a look and I think this is an error in that image.
Not all of the mother's bands will be present in the child's fingerprint as the child's DNA is a mix of the mother and father's DNA not a complete copy of either parent.
I hope that helps.
Why would we separate the dna by size if we can just add an alkali and proceed ?
Great video
Thank you!
Is there a video on gene therapy?
Thanks! ❤
You're welcome 😊
is this edexcel , cambridge , or oxford alevel bio
Hello,
Its specifically AQA Biology.
Thankyou!
You're welcome!
Why is it called genetic fingerprinting tho if you don't necessarily have to use ones finger
good point, it is misleading! The idea is fingerprints are unique and so I a the DNA banding pattern
Thank you
Hope you found it helpful 😊
@@MissEstruchBiology I did thanks- was the only useful resource I could find
Topic 8 makes no sense I swear😭
Its a tough topic, stick at it and keep reviewing the overview mind map to see how it all links
None of the results are the father
Nope, as there is an error in the image with one extra DNA band.