Sequencing at Speed
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- Опубликовано: 15 июл 2024
- This 3D animation gives a simple overview of next generation DNA sequencing, known as massively parallel sequencing.
This 3D animation shows how scientists read the letters in DNA, a process called DNA sequencing. The method shown is known as Next Generation Sequencing or Massively Parallel Sequencing. This is one of the main methods used in sequencing labs around the world.
We start by seeing the DNA being chopped up and modified so that it sticks to the surface if a flow cell. Lots of copies of the DNA fragments are made resulting in millions of small clusters of single stranded DNA. The DNA is then sequenced by adding primers, DNA polymerase enzymes and fluorescently-labelled DNA bases. Lasers pass of the DNA clusters causing the bases causing the fluorescently labelled bases to glow. This fluorescent glow is then detected by a digital camera.
Once this colour has been recorded, the fluorescent terminator tag is removed from the base, allowing a new fluorescently labelled terminator base to be added. The process is repeated and the data exported from the sequencing machine for assembly and analysis.
For more information, please view the video and explore related resources on our site: www.yourgenome.org/video/seque...
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Animated by Polymime Animation Company Ltd.
www.polymime.com
/ polymimestudio 
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I love this channel! Just great! Thank you for all of this...
Nice animation and your voice is a joy to listen to. Well done!
Excellent
Nice video! Just remember this is the principle of Illumina sequencing. Others Next Generation sequencing such as Oxford Nanopore (ONT) are waaay faster and works differen, although with few limitations.
Such a clear and superb demonstration of the methodology. Thank you.
Outstanding video
Awesome!
super explaining👏👏
Can I translate your videos into Uzbek and use them on your channel?
This process sounds from a novice perspective to still be a horrendously inefficient way to read 3 billion pairs from a sequence of 2-bit values. Seems ridiculous to have to cut and replicate and re-bind all those pairs with fluorescent bases and primers. This will certainly one day in the future be sped up to read the sequence in less than a minute.
Definitely, this process takes 4-8 weeks!
Can I ask about application that the video is created on?
wow, genius
How is the DNA assessmbled? How do we know where on the DNA these sequences belong to?
Guys that looks like you are “training” the DNA polymerase enzymes!
Very nice would you please write the translation of what u say in arabic on the video
👍
May i use your video to dub it into urdu
of course I point to the source
Hi.I'm uzbek.
I'm from uzbeksiton.
Só faltou a legenda
24 times the video says DNA. Did I miss any?
We want translation into arabic please ❤
Thanks for great videos❤❤❤❤
Legenda para português
upload video on Genes, complimentary genes lethal genes and epistasis
Hello please add a turkish translatıon
bu kanalı türkçeye çevirip paylaşanlar oluyor genelde
Heart me! Do you think youtube Is good for small Creators? Also, Can we be friends? :D
Vous êtes étudiants en médecine ?🤔 avides d’apprendre et de découvrir quotidiennement pleines de nouvelles choses ?💡 votre insatiable curiosité est votre sacrée qualité mais vous ne savez pas comment l’assouvir ? et bien , vous êtes surement l’abonné recherché soyez la bienvenu parmi nous .😊
Étant convaincus qu’une bonne pratique médicale nécessite des bons acquis et que cela ne vient jamais du jour au lendemain mais demande certainement un engagement quotidien , nous avons eu l’idée de créer ce nouveau concept LE MAJORANT , qui consiste à partager avec vous le MSG FORT DU JOUR (reflexe, note clinique, erreur à éviter...)
Vous êtes prêts à s’embarquer avec nous dans cette aventure ? c’est partie ! 😍