Mitochondrial ATP synthase a subunit structure by cryo-EM and evolutionary covariance

A model of the proton conducting a subunit was constructed by tracing the protein sequence through a 6-7 Å resolution cryo-EM map while satisfying constraints from evolutionary covariance analysis. The green density is from the a subunit, the red density is from the b subunit and the blue density is from subunit A6L. The ribbon diagram represents the a subunit sequence and the red lines show pairs of residues predicted to be in contact be evolutionary covariance. This work is described in detail in Zhou A, Rohou A, Schep DG, Bason JV, Montgomery MG, Walker JE, Grigorieff N, Rubinstein JL (2015). Structure and conformational states of the bovine mitochondrial ATP synthase by cryo-EM. eLife e10180. doi: 10.7554/eLife.10180.