StatQuest: A gentle introduction to RNA-seq

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  • Опубликовано: 23 дек 2024

Комментарии • 566

  • @statquest
    @statquest  2 года назад +6

    Support StatQuest by buying my book The StatQuest Illustrated Guide to Machine Learning or a Study Guide or Merch!!! statquest.org/statquest-store/

  • @Nefglarien
    @Nefglarien 4 года назад +69

    I can't believe it. I was actually crying and panicking when I clicked on the video because of all that stress caused by "I don't know, I don't understand" thoughts. And your intro just killed me XD

    • @statquest
      @statquest  4 года назад +5

      Bam! :)

    • @kristenapostol6288
      @kristenapostol6288 6 месяцев назад +2

      Me too!! I was breaking down internally from exam stress, then i watched the intro and it was like a breath of fresh air :,,,,)

  • @alexanderpalm6407
    @alexanderpalm6407 5 лет назад +159

    I've learned more from this 18-min long video, than my whole course in transcriptomics. You are so good at explaining these complex processes. Thank you!

    • @statquest
      @statquest  5 лет назад +8

      Hooray! :)

    • @Pongant
      @Pongant 4 года назад +3

      I know the feeling. Transcriptomics is so complex that most people, including those working in the field, seem to get "Betriebsblind" and unable to explain what they are actually doing. Josh Starmer is awesome, I just whish we had instructors like that in every research facility.

    • @grace.florejost4611
      @grace.florejost4611 3 года назад +2

      @@statquest same for me, I've understood this concept really easily thanks to you ( better than what I've understood in a 2h genetic engineering class )

  • @himalayanplanespotter2021
    @himalayanplanespotter2021 6 лет назад +207

    Hey Josh, I just started doing my RNA sequencing and your video was very useful for me to get the basic idea behind the RNA sequencing. I spent hours and hours reading papers and didn't understand a thing about it. I really appreciate what you have done. I think your channel will play a big role in my research. Hats off your efforts in coming up with useful informative videos like this.

    • @statquest
      @statquest  6 лет назад +13

      Awesome! That's great news. Since I made that video, I updated my PCA video to be easier to understand, so if you're interested in that, check out: ruclips.net/video/FgakZw6K1QQ/видео.html

    • @Biomeducated
      @Biomeducated 6 лет назад +5

      @Highlander Yeti same for me! Just found out we're going to do RNAseq, immediately went on RUclips search instead of the classical science databases, came out here! I was already looking into (often expensive) courses, but it is all online! This is the future of learning. I should have had stuff like this 12 years ago to study Biomedical Sciences. That's why I'm on a mission helping future/current Biomeds and life scientists discover channels like this. Already BIG thanks from my part, StatQuest! I've yet to check out the rest of your stuff, but I WILL!

    • @jj-zh5mb
      @jj-zh5mb 7 месяцев назад

      Amazing video! Better than any textbooks or even my professor😂

  • @chasecolin22
    @chasecolin22 5 лет назад +26

    First you got me through my statistics course. Then you pulled me through my machine learning courses. Now, you will save me in my graduate genetics lab. Thank you so much Josh.

    • @statquest
      @statquest  5 лет назад +3

      No way! That’s totally crazy. Good luck with your lab! :)

  • @Grillpander
    @Grillpander 5 лет назад +35

    8:48 Two semesters of Bioinformatics and all I wanted was the sequence "GATTACA" to appear on any one slide during a lecture. Thanks, StatQuest for making my wish finally come true!

  • @simafarokhnejad5882
    @simafarokhnejad5882 11 месяцев назад +3

    This is one of the best videos for explaining RNA-seq. You are fantastic. Thank you very much.

    • @statquest
      @statquest  11 месяцев назад

      Thank you! :)

  • @MrChaluliss
    @MrChaluliss 2 года назад +9

    Call this a 'gentle introduction' if you want. It is really just a high level overview of the process. Such presentations are important to building students mental models of any subject, and honestly are frequently overlooked in many subjects in my experience.
    Great stuff.

  • @taysaraujocamilo2313
    @taysaraujocamilo2313 3 года назад +2

    Hey! I'm Brazilian, and I've been learning English and molecular bioinformatics. I'm grateful for this video. Thanks a lot, your pronunciation is easy to understand and you have good didactics too.

    • @statquest
      @statquest  3 года назад +1

      Muito obrigado! :)

  • @alexyang3086
    @alexyang3086 3 года назад +4

    This is the clearest explanation in this sequencing videos. Usually explanation of such technology is hard, but they did a great job.

  • @elfa4231
    @elfa4231 3 года назад +2

    Fantastic video. This is the kind of gentle explanation students (including myself) need. Many thanks from Sweden!

    • @statquest
      @statquest  3 года назад

      Glad you enjoyed it!

  • @bryanl1732
    @bryanl1732 5 лет назад +2

    thank you for making this video so accessible by having captions for everything

  • @helenarg520
    @helenarg520 3 года назад +3

    I think it's great how there's an effort to speak and pronounce clearly; It really helps when you're not a native English speaker!

  • @leyiwang3863
    @leyiwang3863 4 года назад +9

    The intro song really fits me lol. I'm literally crying and watching StatQuest right now.

  • @Silvver
    @Silvver 3 года назад +2

    This is actually one of the easiest-to-understand videos I have ever watched about this subject. Thank you for making it so that I can actually understand the information being taught!

    • @statquest
      @statquest  3 года назад

      Glad it was helpful!

    • @daedrmr2dae
      @daedrmr2dae 3 года назад +1

      @@statquest same comment! patiently explained without assuming we're all experts, written text of what you are saying verbally, simple, pedagogical, and systematic. It's great teaching, and so happy it's free!

    • @statquest
      @statquest  3 года назад

      @@daedrmr2dae Thank you!

  • @paulristeca701
    @paulristeca701 5 лет назад +23

    So the "+" symbol used to be a section where individuals could ad more info about their sequence. This is back when we had far smaller output. Since then, data has grown so much that we have developed tools to go through the data more efficiently. In the end, the "+" is simply an artifact from the early days of these files.

    • @matteobarca1050
      @matteobarca1050 Год назад

      I'm not so sure about that, I think it was like that at the origin of this method, but in the new type of document layout, the + symbol could stand for "+ strand", which is often more investigated than the "- strand" of the double helix.
      I could be wrong, for sure, but I remeber like that

  • @sa19h1j
    @sa19h1j Год назад +1

    Josh Starmer I love you, man. I have been spending hours reading and have only added stress and headaches after
    Thank you so much

  • @PrimacyFromGod
    @PrimacyFromGod 3 месяца назад +1

    Great explanation! I'am bachelor's degree from biology of Universitas Indonesia. It's very useful for me to start learn RNA-seq scope. Big thankyou!!

    • @statquest
      @statquest  3 месяца назад

      Glad it was helpful!

  • @MrsPacmaaan
    @MrsPacmaaan 6 лет назад

    I'm writing my bachelor's thesis about RNA-seq and usage of this method in the viruses life cycle, u helped me a lot in understanding this. Thank you!

  • @asmaakterbhuiyan_shanu3951
    @asmaakterbhuiyan_shanu3951 3 года назад +1

    I am seeing this video in December 2021 and I can't thank you enough for this incredible concept clearing video of RNA-seq. Very Concise and subtle explanation, Besides your ENGLISH is really understandable for other language speakers. I am gonna check another playlist too.

  • @janebond3263
    @janebond3263 4 года назад +1

    You stop my worring , stress and anxiety! thank you God and bless Josh Starmer!

    • @statquest
      @statquest  4 года назад

      Thank you very much! :)

  • @briansalkas349
    @briansalkas349 3 года назад +1

    Less than half way through, already learned more than an entire online course!

  • @norestforthescientist1154
    @norestforthescientist1154 2 года назад +2

    As a scientist with no previous experience in big data (who will need to be tuned in for my new research position), all I can say is THANK YOU.

  • @alexeileliavski732
    @alexeileliavski732 5 лет назад +2

    Very impressed how you explain pseudo-alignment! So clear and so concise! Amazing!!

    • @statquest
      @statquest  5 лет назад +1

      I'm working on a new video that will do a whole analysis pipeline from start to finish and will include pseudo-alignment.

  • @reggie1021
    @reggie1021 4 года назад +1

    Oh wow. Thanks for the explanation! I didn't really understand this concept until now. I've watched this video 3 times now because it is so comprehensive. Thanks a lot.

    • @statquest
      @statquest  4 года назад

      Glad it was helpful!

  • @reflections86
    @reflections86 Год назад +1

    Hey JOSH…First thanks for making such great videos over the years. I see that you even left your job as a professor and have devoted full time to make this stuff since 2017. Appreciate it as it is difficult to break your comfort zone.
    One thing I would say after reading books on mastery is that all elite masters have this skill to explain a tough concept in 2 minutes and they can drag the same concept for hours and hours. From thousands of comments on your videos, majority says you make things extremely simple which is a validation of mastery. The second aspect of elite masters are after working for about a decade and mastering a field then they bring innovation or a new thing (a new concept, theory, a formula, anything that was never explained). I’m sure this would be that phase and stat quest is part of that, and I hope many things would come along with your creativity which you have exploited to make this elite content.
    There are masters but very few have this skill to make things simpler. Many people might comment that you have mastery or talent in statistics or in music but I can say without any doubt that your talent lies is making complex things easier (the medium could either be through a video or through a book don’t matter), but that’s your core talent which you have used. So I wish you keep using this rare talent and bring more contribution to all the humans.
    I will certainly support statquest by purchasing the guides and encourage all who have benefited from this content. Thanks again and lastly is there any email if I could send you a message or share something of value. Will appreciate it.

    • @statquest
      @statquest  Год назад +1

      Wow! Thank you very much!!! BAM! :)

  • @stevensilz-carson1793
    @stevensilz-carson1793 4 года назад +2

    Thank you for this. My current molecular bio professor recommedned we see it before our first class, and it was well worth the 18 minutes!

    • @statquest
      @statquest  4 года назад

      That's awesome! I'm glad you liked the video. :)

  • @steveskalski5363
    @steveskalski5363 6 лет назад +1

    Simple, concise, and straight forward. Well worth the 18 minutes.

  • @leileicui5803
    @leileicui5803 6 лет назад +2

    Bro, super love the various title songs you used for each videos, that attract me to the statistic world! Thank you!

  • @je6874
    @je6874 6 лет назад +2

    This is honestly amazing... the best explanation on RUclips for RNA-seq!!

  • @aditipatel5916
    @aditipatel5916 3 года назад +1

    how grateful i am to the universe to find this 20hours before my interview

    • @statquest
      @statquest  3 года назад

      Good luck! :)

    • @aleksandrat.5058
      @aleksandrat.5058 3 года назад +1

      I am also revising before my interview :D

    • @statquest
      @statquest  3 года назад

      @@aleksandrat.5058 good luck!

  • @maribelyazdanifar8823
    @maribelyazdanifar8823 4 года назад +2

    Thank you for the clear explanations. This was the first time I learned how the machine reads the bases by taking pictures from the top with single base annealing intervals. It was very informative. I am heading to watch your other videos. Many thanks!

    • @statquest
      @statquest  4 года назад

      Glad it was helpful!

  • @yi4763
    @yi4763 4 года назад +2

    The intro song gets me every time ha. But honestly thanks for breaking down the concepts for a newbie like me, keep up the good work

  • @annie0tan
    @annie0tan Год назад +4

    Hey. I'm a clinician trying to do a basic sciences post doc. I haven't touched my biology notebooks in 10 years... and your Video makes it look... like I could maybe, one day, perhaps... UNDERSTAND transcriptomics. (Also makes me feel like an idiot, but in a gentle and kind manner). Thanks

  • @munaalhammadi4237
    @munaalhammadi4237 3 года назад +2

    "+" sign in raw data indicates the forward strand. If paired-end sequencing was performed there will be files with"+" or "-" signs that indicate forward or reverse strands, respectively.

    • @statquest
      @statquest  3 года назад +1

      Thanks!

    • @sdsa007
      @sdsa007 2 года назад

      this makes sense… i think its easy for the machine to figure out in the beginning since probably the oligo adaptors, which bind to the flow cell floor, are probably colour-coded for forward and reverse adaptors, and the adaptors themselves, are also probably colour coded, and the camera can pick up on this at the start, which means the algorithm can interpret forward and reverse strand locations and write it to the file.

  • @patriciamunyadzwe1023
    @patriciamunyadzwe1023 10 месяцев назад +1

    Thank you so much for the video, I have been having headache to grasp the RNA seq concept, now I am set for transcriptomics module.

  • @30tomthomas
    @30tomthomas 6 лет назад +1

    this is the clearest explanation on the internet about rna seq, everywhere else makes it sounds like gobbledygook

    • @statquest
      @statquest  6 лет назад

      Hooray! I'm glad you like my video. :)

  • @cedrichill7499
    @cedrichill7499 5 лет назад +1

    Thanks for this tutorial. It's much better than others I've viewed.

  • @torlarsen2212
    @torlarsen2212 2 года назад +3

    I LOVE gentle intros

  • @sanjanatule
    @sanjanatule 4 года назад +11

    This is an amazing video! I have learnt a lot. We would like to see more videos on bioinformatics.

  • @charlottel9717
    @charlottel9717 5 лет назад +8

    This technique finally makes sense! Thank you so much, this really helped a lot. Very clearly explained and easy to follow.

  • @andromeda3780
    @andromeda3780 4 года назад +3

    Normally I get bored very quickly from explanation but hey this didn't feel like 18 mins at all :D Very useful content and you explain the ideas in pretty well organized way. Thank you that was really helpful ✔

    • @statquest
      @statquest  4 года назад +1

      Thank you very much! :)

  • @Charlottes_WorldWideWeb
    @Charlottes_WorldWideWeb 6 лет назад

    My God Lord this was heaven sent. Any you guys are hilarious! I don't think it's "excessive self promotion" at all, I'm going to watch your PCA one right now!

  • @MarianaFreitas-z1j
    @MarianaFreitas-z1j Год назад +1

    Thank you for this video! It help me a lot, I didn't find anywhere a clear explanation of this content.

  • @dianalauralabastida9166
    @dianalauralabastida9166 3 года назад +2

    This is really well-explained! You got a suscriber! Hope you continue making videos

    • @statquest
      @statquest  3 года назад

      Thank you very much! :)

  • @abinaya276
    @abinaya276 Год назад +1

    you are literally saving my entire life rn

  • @MrErPeeeG
    @MrErPeeeG 7 лет назад +34

    According to the "+" in fastQ, I have heard once, that in this line it used to be a repeat of the sequence name without "@" at the begining, to ensure that quality regards to the sequence above, but to lower file weight it has been switched to the "+". That's what I have heard, but it would be worth to approve somehow this information, I have no sources to do that.

    • @sweboulund
      @sweboulund 7 лет назад +5

      I was taught in my course in metagenome and microbiome analysis, that the + line exists so you can put comments there! Never seen one with comments though.

    • @oliverslay6687
      @oliverslay6687 7 лет назад +2

      MrErPeeeg is correct; but also it signals the end of the sequence lines. FastQ spec: @

      +[]

      A requirement is that the following '+' is optional, but if it appears right after '+', it should be identical to the following '@'. maq.sourceforge.net/fastq.shtml

    • @RamakrishnanRS
      @RamakrishnanRS 5 лет назад +6

      The reason is that FASTQ is a combination of FASTA and QUAL files. Each of those has identifier followed by data. FASTA has sequence, QUAL has quality scores. interleaving these two pieces of information (2 lines from each file "zippered in") gives us 4 lines where 2 of the lines are identical. Later on, when machines got smarter, the third line became obsolete (and expensive space-wise) but is retained in a stunted form for backward compatibility.

    • @saramalik5440
      @saramalik5440 4 года назад

      Too many ads

  • @trannusaran6164
    @trannusaran6164 6 лет назад

    These are great, I’ve been inundating myself with media about genomics and bioinformatics, but a lot of the specific techniques and terminology were still going over my head.
    Thanks for the thorough, but no-nonsense explanations!

  • @taylorshen658
    @taylorshen658 Год назад +1

    this 18-min video is way more concise than 2-years study at Uni.

  • @fifi7244
    @fifi7244 4 года назад +1

    im so glad I found ur channel to potentially save my course

    • @statquest
      @statquest  4 года назад

      Hooray and good luck! :)

  • @chaosdesigner123
    @chaosdesigner123 5 лет назад +2

    I'm just starting my internship for my bioinformatics studies. Just watched the intro and feel so blessed to have a Video like this. So... thanks a lot ^^

    • @statquest
      @statquest  5 лет назад

      Hooray!!! Good luck with your internship. :)

  • @emfazz96
    @emfazz96 7 лет назад +5

    1:27 I love that you did this

  • @hikadani
    @hikadani 7 месяцев назад +1

    i love you. cozy stat vids. this is good for the soul

  • @wissenistmacht6685
    @wissenistmacht6685 2 года назад +1

    This is such a good video. I felt lost until now. Thank you

  • @yimingzhang1251
    @yimingzhang1251 11 месяцев назад +1

    Really helpful video, and extremely easy to understand for beginners, many thanks!

    • @statquest
      @statquest  11 месяцев назад

      Glad it was helpful!

  • @stylutionnet
    @stylutionnet 3 года назад +1

    This is an incredibly brilliant playlist. Thank you sp much for your videos!

  • @johnmandrake8829
    @johnmandrake8829 5 лет назад +1

    someone give this man a medal please

  • @nunitchagucci3047
    @nunitchagucci3047 5 лет назад +3

    Thank you! This is the best video I have ever seen! ❤️

  • @preeti97rox
    @preeti97rox 4 года назад +1

    Your channel is a blessing i might clear an interview after watching all this xD

    • @statquest
      @statquest  4 года назад

      Good luck! Let me know how it goes. :)

  • @ericgathirwa8454
    @ericgathirwa8454 4 года назад +1

    Great description. Thank you.
    Additional information/description about the sequence may be put in the third line that starts with the +

    • @statquest
      @statquest  4 года назад +1

      Thank you very much! :)

  • @liubajc6130
    @liubajc6130 6 лет назад +1

    I loved this explanation!!!. It is the most didactic I have seen of all. Congratulations and many thanks. It was very useful. I really appreciate what you have done.

    • @statquest
      @statquest  6 лет назад

      Thank you so much!!! :)

  • @pepperateash8952
    @pepperateash8952 Месяц назад +1

    Wow this is such a god send for my presentation tomorrow

  • @suhanguo2316
    @suhanguo2316 4 года назад +3

    Love the song! Also, thank you for explaining the fragments part. I didn't know that I don't fully understand the rationale behind fragmentation until you explained it. It's an important side note to understand RNA-seq.

  • @junrancao3896
    @junrancao3896 Год назад

    For 7:30, the + symbol is the orientation I believe. That is, the direction from 3' to 5' or vice versa in which the sequence is read. Great video.

    • @statquest
      @statquest  Год назад

      So is it sometimes a '-'?

    • @junrancao3896
      @junrancao3896 Год назад +1

      @@statquest I want to say a hesitant yes :D I remember reading about orientation representation in NGS somewhere: It is almost universally + because only the forward/reverse strand is kept as one of the final steps of sequencing.

  • @leipingzeng3018
    @leipingzeng3018 6 лет назад +1

    Excellent course! Thank you for making these stuff so clear! It's much easier to understand!

    • @statquest
      @statquest  6 лет назад

      Hooray! I'm glad the video was helpful. :)

  • @NM-tx7zm
    @NM-tx7zm 7 месяцев назад +1

    Your videos help me immensely! Thank you!

  • @mcatprep7053
    @mcatprep7053 5 лет назад +1

    best explanation for this on youtube.... Thanks man. You're the best.

  • @LintonF1
    @LintonF1 4 года назад +1

    Clear, concise, well done! Loved it!

    • @statquest
      @statquest  4 года назад

      Thank you very much! :)

  • @dewisukma5297
    @dewisukma5297 4 года назад +1

    Thank you for sharing, it is very useful to increase my knowledge of RNAseq

    • @statquest
      @statquest  4 года назад

      Glad it was helpful!

  • @renataialchina8887
    @renataialchina8887 6 лет назад +1

    Thank you so much for this very gentle intro to RNA-seq. It helped me with my defense!

    • @statquest
      @statquest  6 лет назад +1

      Hooray!!! Congratulations on your defense! I hope it went well. :)

  • @janebond3263
    @janebond3263 4 года назад +1

    You are totally amaizing! Thank you so so much for so complete explanations . Some details are diff to find or figure out.

  • @piasen9958
    @piasen9958 3 года назад +1

    Hey Josh, amazing content, incredible way of teaching.
    P.S. 'excessive self promotion' killed me! Thanks for being ausum.

  • @quickbiology5159
    @quickbiology5159 5 месяцев назад +1

    wonderful i always wondered how the dot like thing tell you about sequence ,lovely you cleared my basic doubt

  • @fban3735
    @fban3735 2 года назад +1

    Perfect for the lay-person!! Thanks much

  • @bojanristivojevic3396
    @bojanristivojevic3396 6 лет назад

    Thank you so much for this video. It is a great starting point for me, and I have a feeling that I'll be coming back to your channel. Keep up the great work! :)

  • @Thisispow
    @Thisispow 2 года назад +1

    Hey, really good video!
    This was very educational, straight to the point and very much coherent.
    Thanks

  • @brunoguerra4650
    @brunoguerra4650 7 лет назад

    Amazing content ! Easy to understand and complete as possible for YT! Keep it up! Cheers !

  • @bifengxiao9300
    @bifengxiao9300 4 месяца назад +1

    Hi!This might be too late to ask. Firsst of all, thanks for your video, I have no experience with RNA sequencing and struggled to follow through during my bioinformatics class in Graduate school, this video is helping me understand!!! I was wondering what does a sample actually refer to. I though about them as a group of amplified target DNA being sequenced and read. But at 10:55 there is an explanation about bulk RNA sequencing sample that made me confused. Would you mind clarifying this? Thank you very much!

    • @statquest
      @statquest  4 месяца назад

      It really depends. A sample could be RNA from an individual tissue from an individual person, or it could be pooled from that person, or it could be a pool of people. So, "sample" refers to whatever unit the experiment is based on.

  • @jeswang8532
    @jeswang8532 6 лет назад +1

    This is so clear! I'm so happy I checked this. Thank you!

  • @monicasteffimatchado1780
    @monicasteffimatchado1780 4 года назад +1

    Thank you for explaining all the concept in a simple way for easy understanding. Could you please also post a video on pseudo-alignment in RNA-Seq

    • @statquest
      @statquest  4 года назад

      You're the first person to ask about that. I wish I could tell you I would cover it soon. However, I have a long over due promise to make a series on neural networks and another series on time series and mixed models... So it might be a while to get to this. :(

    • @monicasteffimatchado1780
      @monicasteffimatchado1780 4 года назад +1

      @@statquest Thank you so much for the reply. It is a well worth the wait.

  • @Sathishkumar-ru8gc
    @Sathishkumar-ru8gc 3 года назад

    Dear Josh, can you please make a video on small RNA-seq, I think you are the best teacher to explain small RNA-seq in detail.
    Hope you consider.
    Thank You.

    • @statquest
      @statquest  3 года назад

      I'll keep that in mind.

  • @giovannaamaral9019
    @giovannaamaral9019 3 года назад +1

    I just loved it! You made it so simple! Thank u so much!

  • @Sveccha93
    @Sveccha93 6 лет назад +11

    I finally understand like ten things. Thank you!

  • @wonyt1995
    @wonyt1995 Год назад

    As mentioned around the 3-minute mark of the video, it was discussed that when creating cDNA, it is possible to observe fragmentation of RNA. You mentioned that this is a technical limitation. In that case, for example, if there is one mRNA and it is fragmented, the number of mRNAs would increase depending on the number of fragments. I'm curious to know how this issue is being addressed. I'm asking this question now as I've started studying. Thank you.

    • @statquest
      @statquest  Год назад

      There are lots of ways to normalize the number of fragments sequenced per gene. For example: ruclips.net/video/TTUrtCY2k-w/видео.html and ruclips.net/video/UFB993xufUU/видео.html

  • @rrrprogram8667
    @rrrprogram8667 6 лет назад +4

    Ya I even can watch Statquest when I am bored ... Great video

  • @Hozanaa
    @Hozanaa 6 лет назад +1

    Your introduction song made me smile 😄

  • @legendpossible7159
    @legendpossible7159 2 года назад +2

    My report on RNA-Seq is due in 74 hours and I’m just starting this. Fingers crossed.

  • @anmolpardeshi3138
    @anmolpardeshi3138 11 месяцев назад

    at 13:35 when you state that 'we counted the reads per gene' are you referring to read depth - meaning how much (quantity) of the mrna i.e. gene is being expressed?

    • @statquest
      @statquest  11 месяцев назад

      It's a relative quantity.

  • @yiannisconstantinou4834
    @yiannisconstantinou4834 3 года назад +1

    Super helpful and informative, thank you!! You are amazing at explaining things!

  • @kiranraj1553
    @kiranraj1553 4 года назад +1

    This is the best video ever. Thanks a lot!!!

  • @agentgunnso
    @agentgunnso 4 года назад +2

    Yay! thank so much for this video!!! you are excellent!!!

    • @statquest
      @statquest  4 года назад +1

      Glad you liked it!!

  • @josueibarra4718
    @josueibarra4718 4 года назад

    4:15 I have a question: if this is a DNA fragment, shouldn't it be double-stranded? And after, in consequence, when the probes attach themselves to the nucleotides, won't the former attach to nucleotides in both strands? Is this something that is known/on purpose, for example, for increasing the confidence of a read?
    Thank you in advance!

    • @statquest
      @statquest  4 года назад

      In these experiments we denature the DNA to make it single stranded.

    • @josueibarra4718
      @josueibarra4718 4 года назад +1

      @@statquest thank you! Great video btw, very explicative and understandable :)

    • @statquest
      @statquest  4 года назад

      @@josueibarra4718 Thanks! :)

  • @mingweisun1590
    @mingweisun1590 3 года назад +1

    Fantastic explanation! Thank you!

  • @ethanjavan
    @ethanjavan Год назад +1

    thank you so much for the awesome simple explaining

  • @ahsenkonac7992
    @ahsenkonac7992 Год назад +1

    Super helpful and clear! Thanks!

  • @oscarmunoz2269
    @oscarmunoz2269 4 года назад +1

    good explanation and graphics keep up the good work

  • @grsbiosciences
    @grsbiosciences 2 года назад

    Sir, you explained pea is used to reduce the data to plot that means to reduce genes and then you explained pica for samples. Could you please explain clearly sir

    • @statquest
      @statquest  2 года назад

      See: ruclips.net/video/FgakZw6K1QQ/видео.html

  • @dist321
    @dist321 6 лет назад +1

    Thank you very much for your videos! They are indeed clearly explained.

  • @tiberium87
    @tiberium87 6 лет назад

    This is utterly fantastic, thanks a lot for making these!

  • @chumawinnerobiora7749
    @chumawinnerobiora7749 2 года назад +1

    Thank you Sir for this information. you made my day