DNA supercoiling explained

DNA supercoiling explained - This lecture explains about the DNA supercoiling and topoisomerase. it also explains about the positive and negative DNA supercoiling and the role of DNA topoisomerase in changing the DNA supercoiling. Watch this video lecture to understand the following-
1. DNA supercoiling types and how supercoiling works
2. Twist and writhe of DNA supercoiling
3. Role of topoisomerase in DNA supercoiling
For more information, log on to-
www.shomusbiolo...
Get Shomu's Biology DVD set here-
www.shomusbiolo...
Download the study materials here-
shomusbiology.c...
Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in RUclips. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology-
Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store
Shomu’s Biology assignment services - www.shomusbiology.com/assignment -help
Join Online coaching for CSIR NET exam - www.shomusbiology.com/net-coaching
We are social. Find us on different sites here-
Our Website - www.shomusbiology.com
Facebook page- / shomusbiology
Twitter - / shomusbiology
SlideShare- www.slideshare.net/shomusbiology
Google plus- plus.google.co...
LinkedIn - / suman-bhattacharjee-2a...
RUclips- / thefunsuman
Thank you for watching the video lecture on DNA supercoiling and the role of topoisomerase in DNA supercoiling.

[@mahakehail8425]

Every exams on my life university, i remember your lectures to solve the qustions
And you know what? I'm studying master degree now from your videos to understand again and again😁, So thank you very much!
Finally i hope to upload more vidoes in different topics of science, cuz we are depend on your lecture videos
Really thankk u❤

[@zarachowdary6533]

May Allah bless you with huge success and happiness ... ❤❤❤

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@raveenabiswas3333]

Thank so much whenever I am unable to understand by reading books I refer only your video...a huge thanx to you sir ....I am preparing for net exam...Thank you soo much sir

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@user-qy5ol7dh2k]

Thank you so much Sir for your content, even after 6 years it has helped a lot. Honestly I was lazy to learn this topic but after I went through your video I got the stepping stone I needed

[@shomusbiologyofficial]

All the best

[@moinakhan2151]

I recently found your channel and immunology seems to be very difficult for me that's why I asked but now I have found your playlist thanks for telling Sir.
Your way of explaining is easily understandable.
Thanks again

[@shomusbiologyofficial]

+Moina Khan you're welcome

[@rosafarias1565]

You made this so easy to understand, unlike my professor who complicates things way more than he should!!

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@afiasaiyarachowdhury7408]

Thank you so much for this video. I was literally suffering to understand this topic, but you've explained in such a wonderful way. May The Almighty bless you.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@poonamvijaypuriya8815]

One of the best video for such a tough topic.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@Anjali-gl7fz]

When I have doubts my first preference is shomu's biology.....
Thank you so much for clearing my doubts 😇

[@shomusbiologyofficial]

All the best

[@user-xm6ph3tm4s]

You're the best!!!! Thanks a lot!!!! I remember I had emailed you about a certain question like 4 years ago and you answered, I appreciated it a lot, and ever since i have always came back to your channel when it comes to biology!! I just started med school and your channel will help alot!! :)

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@Honeyaami]

sir, you explain it all in a simple way. easy to grasp.. really like your videos.. very much helpful... thanks a lot...

[@sayantandas4505]

Thankyou very much sir, I had doubt on super coiling but seeing your video everything clear. After seeing your video,
It help me to present my presentation on this topic infront of my college teacher

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@masterjiraya1118]

Thanku so much sir I was totally confused in books it is written that lk. Is constant but in example it is changing but u cleared all my doubts

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@monikumari5303]

Sir here your tell us that linking difference = l- l0 but actually it is l0- l is the linking difference i think so... So the. Linking difference us +3 not -3 so it is positive supercoiling 🦹🤔

[@kavyap4453]

Everyone will not have a good teaching skill. You are blessed by god.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@gunjanpatidar8403]

sir amazing I didn't know about this concept before watching your lecture
thankyou sir for clear my doubts

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@nabajitbharadwaj5304]

At 15:48, you wrote that it is positive supercoiling, how can it be +ve, if the Lk is decreased from 9 to 6, according to your discussion it should be -ve supercoiling. I didn't get it.

[@shreyamb6118]

Such a nice lecture ❤️ the concept is clear ...
Thankyou sir ....
I always watch your lectures when I have doubts .

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

[@withlovesanya]

Thankyou soo much you explained it soo easily I couldn't understand a word from book. You are of the best teachers out here.

[@sanjuktaghosh8802]

Thank you so much Shomuda. I had quiet a confusion regarding the writhing during under and overwinding. Your additional example of replication solved it.

[@aarthir2447]

Awesome explanation sir.... Tq 🙏❤

[@shomusbiologyofficial]

You're welcome

[@Dream_and_Achieve]

Thank you so much really helpful ❤❤❤ Confused about it earlier but now crystal clear❤❤❤❤

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@maryum7192]

First lecture and i am impressed just wao... U are Amazing Man❤

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@user-gs2vc6hw1i]

I don't understand unfortunately around 12:23 you said DNA has a right-handed (clockwise) helix having a right-handed supercoil (clockwise). That is right-handed helix structured DNA + right turn causes positive writhe right?
And you said around 19:33 "positive supercoiling = left-handed turn = clockwise direction". But why left-handed? shouldn't it be right-handed as you said before?

[@pijushsingha9232]

Ur r just awesome ur teaching is more better than 3D animation

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@KHUSHBOOKUMARI-hw4zm]

You made me understand this topic very easiest way,,, Thankyou sir

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@anmiann601]

Thank you sir..it is very hard to understand the notes provided from college that's why I watch your lucture....it's very easy to grasp the idea thank you so much sir

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@arshnaazhar1490]

I want to be a teacher like you. Love your way of teaching. 🥺💕

[@shomusbiologyofficial]

Thank you

[@medstunts3168]

video was good but it would have been better if animation was available....i am unable to visualize the process still

[@goldmund2902]

just yesterday i found your channel, whilst studying for a test in my genetics module at the university.
you have an awesome way of explaining stuff, i guess i will come back to this channel many times.
keep up the good work!

[@shomusbiologyofficial]

+Dra'nakyuek, Destroyer of Worlds thank you. Glad to help you out. Stay tuned

[@bushrafouani102]

You are an incredible teacher. Thanks a lot. Best of luck in your career

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@supriyapathak1111]

Thank you so much sir for this wonderful explanation 🙏😇

[@shomusbiologyofficial]

Thank you

[@ZohanaeemRana]

Excellent excellent 👌 explaination method is appreciatable!!!

[@shomusbiologyofficial]

Thank you

[@sawma3714]

Another question - When a supercoil is formed as a result of double helix unwinding, topoisomerases fix that and remove the supercoil but the unwounded helix does not necessarily return to its initial helical state. Thus we get diffence in linking number.
This is how i get an image of DNA supercoiling after watching the video. Is it correct?

[@kmrashmi6464]

You are best teacher
Sir please make video in hindi

[@Naveenkumar-oq2ci]

Super bro i got tensioned, u taught me in simple manner

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@amitdhyani5156]

Easiest way of explaining. Shukriya sir👌✌️

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@shirinkurbanova3511]

thanks dr ı had a cloudy wıth topology ... u r great teacher

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@amina23712]

May God bless you for your effort

[@shomusbiologyofficial]

Thank you

[@DpkTamta157]

Very very thanku so much sir❣️

[@shomusbiologyofficial]

You're welcome

[@Vlogdaily09]

Thanks for this lecture for better and easy understanding.....🙏👍👍

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@SL-my4fg]

right hand-anti clock wise?hoowwww? i cant understand it.

[@rikkigupta9546]

Thanks a lot sir ur all lectures are very informative

[@shomusbiologyofficial]

You're welcome

[@ammarahkapur2389]

Thank you so much sir

[@shomusbiologyofficial]

You're welcome

[@imthadhansar8150]

wow, you are an amazing teacher. God bless you brother

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@negarmokhtari7682]

Thank you very much. It was really helpful

[@shomusbiologyofficial]

You're welcome

[@himadritanayabehera6141]

Very well explained....
Finally understood the theory without animation..

[@pushpankkatare4226]

bhai bahut aacha samzhaya hai maza aa gaya samazh ke

[@shomusbiologyofficial]

You're welcome

[@malik8718]

❤️Thankyou Sir.....

[@shomusbiologyofficial]

You're welcome

[@mgold9999]

As the writhing number (Wr) can be both pos and neg and both increase or decrease the twist number (Tw) as the linking number (Lk) does not change in ccc it's all a matter of the Lk when Wr is 0 which equals Tw and if it's overwound or underwound with respect to Lk naught which is the linking number of fully relaxed DNA under physiological conditions which is usually bp/10.5 and this is what is changed by the Topoisomerases where the Topoisomerase I changes one twist and Topoisomerase II changes two twists (by changing writhes), right? So if delta Lk (Lk minus Lk naught) is negative the DNA structure is negatively supercoiled and has fewer twists when Wr is 0 and if delta Lk is positively supercoiled it has more twists when Wr is 0, right?

[@surajmishra1125]

My professor - Shows confusing animations
me - I have a better option ( Shomu Sir ) :))))

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@fatezurislam3936]

Thank you so much 🫂

[@shomusbiologyofficial]

You're welcome

[@deblinamandal4555]

I can't understand the leacture in my clg class ...... Thanks for this video ...very very needful for me.

[@anisheshdas362]

Thank you

[@shomusbiologyofficial]

You're welcome

[@akeel77]

Thanks so much, I have my exam tomorrow!

[@shomusbiologyofficial]

You're welcome

[@myimmortal9488]

?Right handed supercoiled is negative and left handed is postive in ccDNA why u said the opposite

[@ananyoghosh361]

Its writren in leninger...that right handed helix is positve

[@sudebahar8778]

Thank you so much great explanation and teaching

[@michelehernandez1290]

muchas gracias! me ayudo mucho

[@shomusbiologyofficial]

Thank you

[@kusumqureshi6493]

Sir u r excellent teacher... I understood very well

[@supritadash2535]

Thank u.. so much.. ur lectures always help me..

[@shomusbiologyofficial]

+Suprita Dash Glad you liked my lectures

[@your2321]

Thanks alot

[@shomusbiologyofficial]

You're welcome

[@icsebiology6323]

Thanks for such a fantastic video 🙏

[@shomusbiologyofficial]

You're welcome

[@bareeraarshad2387]

Thanks for such amazing concepts

[@shomusbiologyofficial]

You're welcome

[@bareeraarshad2387]

@@shomusbiologyofficial Respected sir! Can you plz tell your qualification

[@ubaldogennaromastrominico786]

Great lesson...From Italy, my congratulations!

[@shomusbiologyofficial]

You're welcome

[@ubaldogennaromastrominico786]

If anything…Thank you

[@suchit05]

Love u sir❤️

[@shomusbiologyofficial]

Thank you

[@manibharathiduraisamy1344]

Sir nice lecture

[@shomusbiologyofficial]

Thank you

[@sravanigarimella167]

you are explaining things very clear.....but my doubt is clockwise turn of double helix produces negative supercoils ....//// positive ...plz clarify that doubt....and clear many studenyts confusion. (becoz it is useful for many students)

[@prabhleenkaur7756]

I think it produces negative supercoil.

[@purvangparmar16]

Thankyou sir for explaining this topic which is very difficult to understand.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts to help

[@Sambedana221]

Pls make a separate video on topoisomerase

[@shomusbiologyofficial]

Okay

[@pijushsingha9232]

Bro ur awesome

[@shomusbiologyofficial]

Thank you

[@mogalejohn2864]

wow!!! I really enjoyed and learnt a lot of things from this video.

[@shomusbiologyofficial]

You're welcome

[@theresecaparas3377]

What do you think is the importance of the DNA to have a negative and a postive supercoiling?

[@meenublossom6860]

A big thank you... 😊 For explaining supercoiling in such a relexed n easy way 🙏🙏🙏👌👌Awsome lecture ✌️✌️👏👌

[@RubyKumari-mz4co]

Sir plz provide animation vedio for the same...

[@aat85]

You are amazing 🤗👏👏👏👏👏 thank you

[@shomusbiologyofficial]

Glad you liked my lectures

[@aat85]

Your lecture help me with those biology classes and you teach very well 🤗👏 thank you for all the work that you do.

[@user-mo7hl5pi8j]

acha dada bas inhi Ko reply 😊😊

[@Kiwi.an_bean]

Sir I didn't get it full if we talk about counterclockwise writhing,why doesn't the number of twists decrease?
Dna here has right handed twisting originally and upon being negatively super coiled why does its twists increase, shouldn't they decrease as it'll open the twisting??

[@reshmapremjith3239]

Superb class

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@rukhsarjeandani9247]

your voice is hardly audible but you explained it very well

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@mirajmohammad1169]

Thank you sir...😊 Great to learn from you.

[@shomusbiologyofficial]

Glad to hear that you are getting benefit from the videos

[@sadiakhalid3109]

Before watching video i like your video

[@shomusbiologyofficial]

Thank you

[@ayeshaashraf1996]

super amazing 💓

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@patarwalparveen]

Your videos are always amazing..easy to understand. Please upload videos on diseases asked in NET exam

[@user-rw7bm7nn5h]

Thank you sir

[@shomusbiologyofficial]

You're welcome

[@rezanur356]

it was a wonderful explanation

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@lakshmiparvathy5826]

Can't thank u enough❤️

[@shomusbiologyofficial]

Don't thank just like and share

[@chandanhalder7215]

Thanks a lot sir🙏☺

[@shomusbiologyofficial]

You're welcome

[@nehabhandari7747]

You made it super easy sir ji :)

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@ashutoshs1966]

I really appreciate the efforts..
But at 12:35 u said right handed coill means clockwise turn..is that correct?

[@ankitasamal8067]

Best lecture on this topic✨

[@shomusbiologyofficial]

Thank you

[@swastikapatra6958]

Sir left-handed supercoiling is positive and right-handed is negative so the writh will be positive in left n negative during right

[@chashabhusho]

Will you please explain how twist is decreased during separation of a closed circular DNA?

[@mmmpr2261]

I feel like...I owe you so much!

[@Biologywithjoshisingh]

Thnku sir

[@shomusbiologyofficial]

You're welcome

[@dakomaz5709]

god bless you shomu

[@shomusbiologyofficial]

Thank you

[@anjalikushwah4448]

Superb sir

[@azrathaseen]

Amazing lecture learnt a lot !!!

[@shomusbiologyofficial]

+Azra Thaseen glad to hear that

[@Manikalens]

Sir it was really helpful... Thankuu

[@mitalgangrade213]

Sir..... Plz clear my doubt
Earlier in video u said DNA is right handed so if rt handed helix coiled in rt then wr will be positive
And later in video u said positive supercoiling is always left sided.... Plz help to clear concept sir

[@gayatribhatt4869]

Thanku so much sir ... 😇

[@shomusbiologyofficial]

You're welcome

[@muhammadzuabi]

I think the LK should be 10 according to the drawing and the definition. Did I miss something?

[@oscarniiottodarko4510]

me too i also think it should be 10

[@manasranjan700]

Absolutely ...