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thank you so much sir for nice explanation. Sir i want to ask you why we use imidazole for purification in this technique what is the major role and how it works?
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
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Thank you for the easy explanation..can you speak about Fusion tag protein;Fc and suitable elution buffer since it is large molecules
Well explained! Keep making such videos
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no mention of imidazole competitor or chemistry
Very good video 😀
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thank you so much sir for nice explanation.
Sir i want to ask you why we use imidazole for purification in this technique what is the major role and how it works?
Imidazole competes with the his-tag for binding to the metal-charged resin and thus is used for elution of the protein from an IMAC column.
Thank you so much 💓
شكرا جزيلا
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
How basic buffer pH affects the binding of histidine tagged protein in Ni-NTA column?
If there is low pH, the Histidine residues will be protonated and thus are not binding to Ni2+ resin anymore. Let me know if it's helpful.
Can you give me the refrence of your information
Thanks you for this video
This vidio help me for drug discovery.
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very helpful video thank you so much. can you also provide it as a ppt file or pdf somewhere so we can use it and keep it? thank you
Ppt is chargeable ($5) and pdf ($2). If you are interested let us know via email. Arpanparichha1994@gmail.com is the channel email
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