His tag protein purification | Application of his tag purification | Affinity chromatography

In this video, we will talk about His tag protein purification in detail and discuss its application. this falls under Affinity chromatography.
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[@aljenembtry7781]

Can't wait!!!

[@artpage6163]

Thank you for the easy explanation..can you speak about Fusion tag protein;Fc and suitable elution buffer since it is large molecules

[@samriddhidubey2417]

How basic buffer pH affects the binding of histidine tagged protein in Ni-NTA column?

[@vv-lx4se]

no mention of imidazole competitor or chemistry

[@MolecularMedicine327]

Can you give me the refrence of your information
Thanks you for this video

[@user-jq6bw4hh2h]

thank you so much sir for nice explanation.
Sir i want to ask you why we use imidazole for purification in this technique what is the major role and how it works?

[@animatedbiologywitharpan]

Imidazole competes with the his-tag for binding to the metal-charged resin and thus is used for elution of the protein from an IMAC column.

[@bharatupadhyay9216]

Thank you so much 💓

[@Suraj_gawali]

This vidio help me for drug discovery.

[@animatedbiologywitharpan]

Please share with friends

[@pani6697]

very helpful video thank you so much. can you also provide it as a ppt file or pdf somewhere so we can use it and keep it? thank you

[@animatedbiologywitharpan]

Ppt is chargeable ($5) and pdf ($2). If you are interested let us know via email. [e-mail] is the channel email

[@armani4656]

Very good video 😀

[@animatedbiologywitharpan]

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