Autoclave = Sterilization Process of Glassware & Nutrient Media by Moist Heat Sterilization (HINDI)

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  • Опубликовано: 11 сен 2024
  • This video is also available in English Autoclave- General Construction and Working Principle- Moist Heat Sterilization (English). • Autoclave | General Co...
    Download "Solution Pharmacy" Mobile App to Get All Uploaded Notes, Model Question Papers, Answer Papers, Online Test and other GPAT Materials - play.google.co...
    Autoclave- General Construction and Working Principle- Moist Heat Sterilization (English)
    The autoclave is a container similar to a pressure cooker. It is filled with the objects to be sterilised and then sealed. Next, high-temperature steam is forced in under high pressure, thereby displacing air. Moist heat destroys microorganisms by the irreversible coagulation and denaturation of enzymes and structural proteins. The time and temperature to accomplish this depending on pressure and the type of microorganisms to be inactivated. After the necessary time has passed, the steam is released and the sterilised objects are removed. The entire cycle can take from 15 to 60 minutes (batch processing).
    A more commonly used method when extended heat is not a concern is to use an autoclave or pressure cooker. When sterilizing in this way, samples are placed into a steam chamber on a shelf or raised floor, and the chamber is closed and heated so that steam forces air out of the vents or exhausts. The pressure is then applied so that the interior temperature reaches 121 °C (250 °F), and this temperature is maintained for between 15 and 30 minutes. This elevated temperature and pressure are sufficient to sterilise samples of any commonly encountered microbes or spores.
    This video will help you to understand- autoclave working, the principle of an autoclave, sterilization process, the importance of sterilization, sterilization process, moist heat sterilization, working of an autoclave, construction of autoclave, how autoclave works,
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Комментарии • 104

  • @shraddhadubey4276
    @shraddhadubey4276 3 года назад +30

    Ur voice directly penetrates to the brain clearing the whole topic.!!! Thank you mam.

    • @premwele9440
      @premwele9440 2 года назад

      Yes mam I also like your teaching explanation thanks mam

  • @Indrajit-DN94
    @Indrajit-DN94 Год назад +10

    Visual perception is so much more effective
    Thanks a lot

  • @sudhanshuranjan1881
    @sudhanshuranjan1881 5 лет назад +16

    Mam and sir you are the best.. you are doing invaluable job for students... thanks

  • @jinalvanjara5240
    @jinalvanjara5240 Год назад +4

    Thankyou tommorow is my exam n l missed this practical during classes
    Big help😊❤️

    • @mayankrai772
      @mayankrai772 2 месяца назад

      Isse achha me bata sakta hu

  • @vandanaprajapat9656
    @vandanaprajapat9656 Месяц назад +1

    Thank you for this video... video is very helpful.... thank you so much 😊

  • @hazarikahemanta3760
    @hazarikahemanta3760 4 года назад +1

    Bohot atxa laga vedio ko dekh kr or atxyei se samas me a gaya..tqq

  • @ShahzadMalik-of2ox
    @ShahzadMalik-of2ox 14 дней назад +1

    maam thanks for excellent knowledge after sterillization is complettedis chamber is emptied from water?how to do it?and where this water should be sent? kind regards

  • @Automationstudy
    @Automationstudy 6 месяцев назад +1

    Very nicely explained

  • @crazyqueen.7212
    @crazyqueen.7212 9 месяцев назад +1

    Thank you so much mam..
    U are a saviour❤

  • @shoyabmasuldar2856
    @shoyabmasuldar2856 3 года назад +1

    Very nice, understanding me sooo much tq😊👍👍

  • @aashishraj3620
    @aashishraj3620 4 года назад +1

    Thankyou for tjis video book is boring..
    Video is very interesting and informative thank you

  • @bipss3365
    @bipss3365 Год назад +1

    I'm obliged🙏🏼🙏🏼

  • @ruchika_899
    @ruchika_899 Год назад +1

    Tysm ma'am 💓

  • @gururajagvt1582
    @gururajagvt1582 2 года назад +2

    Thank you somuch

  • @dhanvindpk8276
    @dhanvindpk8276 3 года назад +3

    Very neatly explained..thank you

  • @bilalahmad6102
    @bilalahmad6102 5 лет назад +1

    nice good job help full video ma'am thnks.. 👍

  • @poojakamble7292
    @poojakamble7292 Год назад +1

    Thank you so much mam 🙏😊

  • @Satendra.maurya
    @Satendra.maurya Год назад +1

    thanks

  • @mamta5026
    @mamta5026 3 года назад +1

    Nyc mam guiding

  • @aarzoofiza700
    @aarzoofiza700 Год назад +1

    Helpfull❤

  • @user-bo7bh5lt1m
    @user-bo7bh5lt1m 5 лет назад +1

    Thank you for uploading this video

  • @KuldeepKumar-tn2bo
    @KuldeepKumar-tn2bo 4 года назад +1

    Very nice

  • @artistrajesha.r.k.v6442
    @artistrajesha.r.k.v6442 4 года назад +1

    Thanks ma'am

  • @shaiklathifmunnisha1426
    @shaiklathifmunnisha1426 2 года назад +1

    Tq so much mam

  • @kiranpal4348
    @kiranpal4348 Год назад +1

    Thank u mam

  • @rahulporadhiya3032
    @rahulporadhiya3032 3 года назад +1

    Thanks madam

  • @Dearlazytraveller
    @Dearlazytraveller 3 года назад +1

    Steam release tight karna ki loose?

  • @Nikii-qz9xc
    @Nikii-qz9xc Год назад +2

    Kon kon mlt se h 😗
    Vo like karo

  • @forknowledge9162
    @forknowledge9162 4 года назад +1

    Nice video

  • @PreetiSharma-bi4me
    @PreetiSharma-bi4me 3 года назад +2

    What about vacuum releasing vent

  • @vaishnavigopaltupkar2306
    @vaishnavigopaltupkar2306 4 года назад +2

    Very helpful 😍 tysm mam

    • @rajnipuri912
      @rajnipuri912 Год назад

      Where to get gasket for this autoclave

  • @BITTUKUMAR-eb9oy
    @BITTUKUMAR-eb9oy 5 лет назад +1

    Very nice mam

  • @mohanrock5810
    @mohanrock5810 4 года назад

    Nice voice....

  • @KuldeepKumar-tn2bo
    @KuldeepKumar-tn2bo 4 года назад +1

    ❤️❤️❤️❤️Good

  • @rsingh1508
    @rsingh1508 3 года назад

    Thanks broh

  • @onlyislamytch
    @onlyislamytch 3 года назад +1

    Should we use distilled water in autoclave? or the tap water?

  • @aventadorgamer1575
    @aventadorgamer1575 3 года назад +1

    Helpful

  • @gowthamib1766
    @gowthamib1766 2 года назад

    Thank you but I have a small doubt how many whistle s came out

  • @simon1793
    @simon1793 3 года назад

    Mam, mera whoicel kam nehi korta.but sefty valve sey pressure bahar hota hey 15 psi may. Same autoclave. Hoi problem hogi?

  • @Rajeevkumar-dx2up
    @Rajeevkumar-dx2up 5 лет назад +1

    Nice vedio

  • @nicoledelas-alas319
    @nicoledelas-alas319 2 года назад +2

    Can you autoclave lab materials and cultures at the same time?

  • @rahulporadhiya3032
    @rahulporadhiya3032 3 года назад

    Mam thanks

  • @bikashsahoo6539
    @bikashsahoo6539 2 года назад

    More videos about sterilization

  • @tusharrajput7850
    @tusharrajput7850 Год назад

    Mam gauze piece ko kitane temperature pe sterilize khota hai?

  • @julisharkar3315
    @julisharkar3315 5 месяцев назад +1

    Anyone from Shree Janagapati ❤

  • @rishabhchourasia5697
    @rishabhchourasia5697 Год назад

    which autoclave is this and where can I buy it? Can you please help me ma'am.

  • @jagannathhalder9822
    @jagannathhalder9822 3 года назад +1

    Autoclave price kya hai ?

  • @sadhnakalra9957
    @sadhnakalra9957 2 года назад

    Kha milega ye mai Faridabad se hu iski jrurt hai mujhe

  • @surajchougule4351
    @surajchougule4351 2 года назад

    What is autoclave size ltrs ?

  • @namitadewan3001
    @namitadewan3001 4 года назад

    How to know when the article is sterlised. For how much time do we have to to keep the article in this autoclave..

    • @solutionpharmacy
      @solutionpharmacy  4 года назад

      This details are given in standard pharmacopoeia and we have to follow as per object.

  • @shyamagrawal36
    @shyamagrawal36 5 лет назад

    Sir water bath kise kahte hai and isse vaccine ka sterlization hota hai kya

    • @solutionpharmacy
      @solutionpharmacy  5 лет назад

      Shyam Agrawal Water bath ek aisa chamber hai jisme pani ko garam karke ek temp par heat kia jata hai. Vaccine ka nahi par iska upyog dusre purpose ke liye kia jata hai

  • @techritvik6064
    @techritvik6064 7 месяцев назад

    Kon kon se material ko sterilization ke liye autoclave me

  • @M04-Orhan
    @M04-Orhan 3 месяца назад

    Dmlt walo kya haal hai

  • @agmpublicschool
    @agmpublicschool Год назад

    Price kya h

  • @beinghuman4457
    @beinghuman4457 4 дня назад

    temperature kaise pata chalega ??

    • @solutionpharmacy
      @solutionpharmacy  4 дня назад

      Is wale me temperature nahi pata nahi chalta

    • @beinghuman4457
      @beinghuman4457 4 дня назад

      @@solutionpharmacy fr kaise bol sakte hain ye specific temperature pe operate ho raha hai ?
      for example we usually use 121 degree ,15psi for 20 minutes
      jab temp he ni dikh raha toh fr how things work ?

  • @sumairaraza8811
    @sumairaraza8811 3 года назад

    Madam is ko ab start krna bhh bta den

  • @Daytodaytimepass
    @Daytodaytimepass 4 года назад

    how many rubees mam

  • @amitingle6025
    @amitingle6025 4 года назад

    Can we add any disinfectant solution in autoclave

  • @SunitaSharma-ew3fl
    @SunitaSharma-ew3fl 4 года назад

    Aoutocleve machine Jaipur me khan milti h or Kitne rupees Ki sabse sasti machine Kitne ki h

  • @mahadabdi4158
    @mahadabdi4158 3 года назад

    English subtitles plz

  • @mehtajaydip6884
    @mehtajaydip6884 4 года назад

    Why emulsion are not sterilized in autoclave?

  • @himanshu9414
    @himanshu9414 4 года назад

    पका दिया
    इसे ऑपरेट कर के बताते.तो अच्छा होगा..
    वीडियो थोड़ा छोटा होना चाहिए.
    और

    • @solutionpharmacy
      @solutionpharmacy  4 года назад

      Dear sir. This was an demo of parts as mentioned on title. Sorry for the length for you but for a new and avg level student is was required.

  • @KiranYadav-qc3cl
    @KiranYadav-qc3cl 2 года назад

    explain in English

    • @solutionpharmacy
      @solutionpharmacy  2 года назад

      Also available in English. Please check in channel

  • @dora293
    @dora293 2 года назад

    Why you put English 😕

    • @solutionpharmacy
      @solutionpharmacy  2 года назад

      Sorry sir by mistake that was written as we have uploaded same video in English. Now corrected. Thank you for your information. Sorry again

  • @HindustaniDil-tu8eq
    @HindustaniDil-tu8eq 8 месяцев назад

    J

  • @DhirajKumar-nj2xi
    @DhirajKumar-nj2xi 7 месяцев назад

    Hindi

    • @solutionpharmacy
      @solutionpharmacy  7 месяцев назад

      Pahle se Hindu me bhi hai, please search in channel

  • @JatinderSingh-uk6og
    @JatinderSingh-uk6og 4 года назад +1

    Bekar and risky

    • @solutionpharmacy
      @solutionpharmacy  4 года назад

      Sorry. It not for playing its peactical. Is druving bike or using mobile is 100% safe ?

  • @futuremotivation9266
    @futuremotivation9266 Год назад

    Thank you ma'am

  • @_Aasiya_
    @_Aasiya_ Год назад

    thank you so much mam 💖