Autoclave = Sterilization Process of Glassware & Nutrient Media by Moist Heat Sterilization (HINDI)
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- Опубликовано: 11 сен 2024
- This video is also available in English Autoclave- General Construction and Working Principle- Moist Heat Sterilization (English). • Autoclave | General Co...
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Autoclave- General Construction and Working Principle- Moist Heat Sterilization (English)
The autoclave is a container similar to a pressure cooker. It is filled with the objects to be sterilised and then sealed. Next, high-temperature steam is forced in under high pressure, thereby displacing air. Moist heat destroys microorganisms by the irreversible coagulation and denaturation of enzymes and structural proteins. The time and temperature to accomplish this depending on pressure and the type of microorganisms to be inactivated. After the necessary time has passed, the steam is released and the sterilised objects are removed. The entire cycle can take from 15 to 60 minutes (batch processing).
A more commonly used method when extended heat is not a concern is to use an autoclave or pressure cooker. When sterilizing in this way, samples are placed into a steam chamber on a shelf or raised floor, and the chamber is closed and heated so that steam forces air out of the vents or exhausts. The pressure is then applied so that the interior temperature reaches 121 °C (250 °F), and this temperature is maintained for between 15 and 30 minutes. This elevated temperature and pressure are sufficient to sterilise samples of any commonly encountered microbes or spores.
This video will help you to understand- autoclave working, the principle of an autoclave, sterilization process, the importance of sterilization, sterilization process, moist heat sterilization, working of an autoclave, construction of autoclave, how autoclave works,
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Ur voice directly penetrates to the brain clearing the whole topic.!!! Thank you mam.
Yes mam I also like your teaching explanation thanks mam
Visual perception is so much more effective
Thanks a lot
Mam and sir you are the best.. you are doing invaluable job for students... thanks
SUDHANSHU RANJAN Thank you so much for your valuable feedback. Its motivate us to give our best
5d5 5
Thx
Thankyou tommorow is my exam n l missed this practical during classes
Big help😊❤️
Isse achha me bata sakta hu
Thank you for this video... video is very helpful.... thank you so much 😊
Bohot atxa laga vedio ko dekh kr or atxyei se samas me a gaya..tqq
maam thanks for excellent knowledge after sterillization is complettedis chamber is emptied from water?how to do it?and where this water should be sent? kind regards
Very nicely explained
Thank you so much mam..
U are a saviour❤
All the best
Very nice, understanding me sooo much tq😊👍👍
Thankyou for tjis video book is boring..
Video is very interesting and informative thank you
Thank you
I'm obliged🙏🏼🙏🏼
Tysm ma'am 💓
Thank you somuch
Very neatly explained..thank you
nice good job help full video ma'am thnks.. 👍
Thank you so much mam 🙏😊
thanks
Nyc mam guiding
Helpfull❤
Thank you for uploading this video
Most welcome
Very nice
Thanks ma'am
Tq so much mam
Thank u mam
Thanks madam
Steam release tight karna ki loose?
Kon kon mlt se h 😗
Vo like karo
Nice video
Thank you
What about vacuum releasing vent
Very helpful 😍 tysm mam
Where to get gasket for this autoclave
Very nice mam
Thank you
Nice voice....
❤️❤️❤️❤️Good
Thanks broh
Should we use distilled water in autoclave? or the tap water?
You can use normal water
Helpful
Thank you
Thank you but I have a small doubt how many whistle s came out
Mam, mera whoicel kam nehi korta.but sefty valve sey pressure bahar hota hey 15 psi may. Same autoclave. Hoi problem hogi?
Nice vedio
Thank you
Can you autoclave lab materials and cultures at the same time?
Yes but cover the lab material's glass ware with aluminium foil
Mam thanks
More videos about sterilization
Mam gauze piece ko kitane temperature pe sterilize khota hai?
Anyone from Shree Janagapati ❤
which autoclave is this and where can I buy it? Can you please help me ma'am.
Autoclave price kya hai ?
No idea. We are actually not seller
Kha milega ye mai Faridabad se hu iski jrurt hai mujhe
What is autoclave size ltrs ?
How to know when the article is sterlised. For how much time do we have to to keep the article in this autoclave..
This details are given in standard pharmacopoeia and we have to follow as per object.
Sir water bath kise kahte hai and isse vaccine ka sterlization hota hai kya
Shyam Agrawal Water bath ek aisa chamber hai jisme pani ko garam karke ek temp par heat kia jata hai. Vaccine ka nahi par iska upyog dusre purpose ke liye kia jata hai
Kon kon se material ko sterilization ke liye autoclave me
Jo heat aur pressure withstand kar paye
Ok 🤍
Dmlt walo kya haal hai
Price kya h
temperature kaise pata chalega ??
Is wale me temperature nahi pata nahi chalta
@@solutionpharmacy fr kaise bol sakte hain ye specific temperature pe operate ho raha hai ?
for example we usually use 121 degree ,15psi for 20 minutes
jab temp he ni dikh raha toh fr how things work ?
Madam is ko ab start krna bhh bta den
how many rubees mam
Can we add any disinfectant solution in autoclave
Not required. It may cause staining
Solution- Pharmacy ok.. thankyou
Aoutocleve machine Jaipur me khan milti h or Kitne rupees Ki sabse sasti machine Kitne ki h
Order karana padta hai..
English subtitles plz
Video is also in english. Please find
Link given in description box
Why emulsion are not sterilized in autoclave?
Heat sensitive and phase separation.
पका दिया
इसे ऑपरेट कर के बताते.तो अच्छा होगा..
वीडियो थोड़ा छोटा होना चाहिए.
और
Dear sir. This was an demo of parts as mentioned on title. Sorry for the length for you but for a new and avg level student is was required.
explain in English
Also available in English. Please check in channel
Why you put English 😕
Sorry sir by mistake that was written as we have uploaded same video in English. Now corrected. Thank you for your information. Sorry again
J
Hindi
Pahle se Hindu me bhi hai, please search in channel
Bekar and risky
Sorry. It not for playing its peactical. Is druving bike or using mobile is 100% safe ?
Thank you ma'am
thank you so much mam 💖