Reverse Transcription PCR

( www.abnova.com ) - Reverse transcription PCR includes two steps. The first step is reverse transcription, in which RNA is reverse transcribed to its DNA complement (complementary DNA, or cDNA) using reverse transcriptase and primers. The second step is amplification using traditional or real-time PCR. More videos at Abnova www.abnova.com

[@weqaclient7770]

(review follows) here's a very nice video of a human hand doing a series of things with a precision pipetter and some small titration tubes. From the distance (and camera lens used), it is completely impossible to garner any sense of what is happening, other than to read the text. This generally makes this a text reading exercise with nice background music.
for any given moment, is something going in or coming out ? here's where we change pipetter tips to avoid cross contamination, here's where we put in the "ingredient x"... try replacing all the captions with the words: here's me trying out my new pipetter i got for xmas !!! and see if the video is one tiny bit more or less informative.

[@jaspingo]

I completely agree. Trying to research RT-PCR and I'm finding it difficult. Thought I'd try watching videos but without captions, I have no idea what's going on.

[@genesisdayrit1]

this beat goes hard

[@francois-xaviersicot1088]

happy to see Takara Thermal Cycler Dice at work :)

[@howabtme]

the machine used in both 2 steps is the thermalcycler right?

[@SpillFreakMusic]

why is rnase used seperately if depc water is already in use?
if we use normal water we can already get the rnase,

[@Lifeofapie]

quick question: why does RNA initially need be denatured? It's already single stranded- ready for the reverse transcription process.

[@Lifeofapie]

ahhh I see. Good point! :)

[@oliviapereira364]

@@Lifeofapie I have that question too, what answer did you get? Thanks!

[@user-cv7lk1yj4c]

Pretty sure it's cos the RNA has probably formed secondary structures (bound to itself) so the high temperature will break the hydrogen bonds it's made between its own bases

[@russelmiller7311]

Nice work! Very informative...

[@78rydwan]

24 thousand viewers , but no comments! come on guys!

[@heysselg.3993]

Syed Ali scientists are very inquisitive and terribly shy creatures. Please don’t scare them...

[@user-qd6el6dc3x]

4:11 how i get this time cycle nimber???

[@skdavidnba]

what is a reverse transcription reaction mix??

[@victorianpunx00]

I wish there was an explanation of the purpose of RT-PCR, nice music though :)

[@jeanfernandez5397]

The purpose is to reveal the genes that express the mrna being reverse transcribed.
And I know, four years ago...lol

[@inamahdi7959]

I know I am a year late, but if you know the RNA sequence, can't you figure out the DNA seq? or do are we making (figuring out the RNA sequence) the RNA using primers?

[@omarrashedsamarraie9466]

@@jeanfernandez5397 hallo plaes halp

[@nunzinho91]

Nice work :)

[@kingsleydunkley9562]

U

[@toxicbrownies]

pooop

[@1TyroneBiggums]

poop