😂 I was literally going to say that exact same thing man. I wasn't even going to watch this video because I already know this technique but after I seen the wet paper towel with the silicone I said well damn I guess my theory is right that you can learn something from everybody. Kudos, my friend. Thank you for making a video for all of us ignorant mushroom lovers😂😊.
Love your paper punch hack for the holes. I've been drilling them and the burr can be a pain to clean up. If you push lightly from the inside with the wet paper towel on your very last massage of the RTV blob you will get a nice raised convex area exactly above the whole. Like a Bull's Eye for the syringe needle.
Sir, I appreciate your videos. May I suggest that you use the new cheap, thin Chinese snap lids for this purpose, as they are So thin as to be questionably functional for canning purposes. They will be much easier to punch.
Is there a specific reason you use a hole punch to make the holes or can the same results be achieved with a drill or a hammer and nail or something else?
@@SporenSprout❤ thank you so much! I decided to order the injection ports but I was mostly concerned about the mocropore tape but would that then eliminate the need to unscrew the lids when under pressure?
@@Klinezilla I still unscrew the lids a bit while using the 2 layers of micropore tape and pressure cooking but yeah it’s not really needed for grain jars. With LC if you don’t unscrew a bit the liquids will splash up and hit the get the tape wet under pressure. Wet tape can cause contamination.
i was about to say that is the sole purpose of using foil in both a PC and or instapot along with the broke boi -popcorn tek boil method ( i dont use this) i prefer the instapot have had good results so far@@SporenSprout
@@SporenSprout ig the paper tape isnt bothered during pressure cooking or steam bathing ur jars? Meaning the adhesive doesnt lose any hold or degrade during all that heat?
@@NightWitch1630 two layers of tape and some aluminum foil over top of the jars (protects from steam seeping through tape) while pressure cooking and you're golden.
I've mostly seen the air exchange hole filled with polyfill. Is paper tape alright too? On shroomery a few users were saying micropore tape here is much worse than polyfill.
I’ve made a very large number of liquid cultures like this and I’ve never had a problem. 2 pieces of 3M tape works everytime. Just make sure to keep the jar high off the ground. You don’t want a bunch of dust settling on the lid. You can wrap a coffee filter around the lid for extra dust protection if your room is dusty. The cool part about this hobby is that you can do whatever works for you, so feel free to experiment with whatever is easiest for you to access.
Yeah I would use 2-4 pieces. It does prevent contamination especially if you can keep it from getting wet and prevent dust from landing on the lid over time. If you want more security you should use waterproof synthetic filter discs instead.
Why red RTV and not gray. I’ve seen both but wasn’t sure if it was temp related. I’ve got a few tube of the gray that are unopened and all ready paid for by some other project
Grey RTV has higher density when cured for higher vibration resistance and tight tolerance applications. Red RTV is for exhaust and high heat applications. Gray will probably work fine but I have not used it.
If you wanted to get crazy, you can just inoculate through a hole covered with micropore tape and then just put another layer of tape over the hole very very fast and in a clean environment.
They take 2-3 hours to dry but as soon as the Rtv is not sticky anymore you can use them and the pressure cooker will finish solidifying the Rtv. They hold up very well, and you can add more than one injection port if you want in the future.
My surface is perfectly clean (cleaned with acetone) but the silicone peels right off after drying... this is useless. Going back to self adhesive injection ports... much easier to apply and stays on.
@@SporenSprout How? From what I read, silicone doens't stick to metal or plastic. I have tried, sanding the surface, clean the surface etc. It still peels off just by pickig it with finger nail.
Hey spore! My spots of silicone are no longer sticky but they still smell really strongly; will the grains be safe? I put the lids on the jars to carry through to another and when I took them off it smelt really really strong. Just wanted to check before putting my grains in the pc!
When I squeeze out the silicone, the lid is set on top of a lid ring and that’s where it cures for around 4 hours and then can be pressure cooked. If you mean that you let the silicone cure while set on top of a jar, the bottom silicone probably is still wet because no air got to it. The smell of silicone isn’t a big deal so I wouldn’t worry about it.
Do u shake and break with this method? Doing my first inoculation tomorrow and I read somewhere that if u shake the jars, if any of the grains touch the microtape it could potentially cause contamination. Let me know what ya think!
@@SporenSprout Thank you! I ordered your waterproof filter disks from your Etsy store! 🙂 Happy to support your store! Thanks for such a great explanation. I'm enjoying watching all your vids. Peace and thanks!
After the silicon drys it’s ready to go. Sometimes a tiny piece of silicon will get stuck in the needle when injecting and end up inside your liquid culture. If you insert the needle at an angle with the opening of the needle end facing up and then straighten normally, it works better for me. You shouldn’t have a problem with the tiny red piece of silicon causing contamination.
Am I still good to shake my grain jars when they're partially colonized? And do you keep tin foil on through the colonization process or just until they're inoculated?
@@sethhaze1610 no problem! The silicone will usually withstand around 6-10 injections before becoming compromised. It’s easy to replace the silicone or dispose of the lid entirely if it starts to rust. I’ve been using these lids religiously for years and I love them, good luck!
Yes you can use a lighter without causing damage. 18 gauge needle works well, I wouldn’t go any thicker than that. If the needle is brand new out of the sterile package I only use ISO
Have you ever tried just injecting through the paper tape and immediately covering the injection sight with another piece of tape? This seems easier in my novice rookie brain.
Tighten the lid ring and then loosen it a quarter turn. Sometimes I forget to loosen it a bit and it’s not really a big deal. The micropore tape might come loose a little so just make sure the tape is secure after.
That happens to me a lot and never causes problems but I do try to avoid that. It’s more of a concern if after months of sitting, the liquid splashes up and hits the tape.
@@SporenSprout Ok thank you! I was thinking that once my LC is ready I'll transfer in 10ml syringes and store them in the fridge like that, is it better than keeping it in the jar?
@@SporenSprout thank you for sharing this. I am trying my first grow using this method. do you use foil as well in the pressure cooker along with the tape?
Micropore tape before cooking, remember your aluminum foil. Protects the lid from moisture. It doesn't hurt to do two layers of tape. I always do, make a little x.
I don’t understand the point of having a sealed injection port if there is a paper tape vent where air can freely move in and out ? Not being a dick. I honestly don’t understand ?
The simplest Methode: (German...dolmetsch this!)Kauft Teppichklebeband.Schneidet so tief ein daß ihr 2Lagen runterschneidet.Auf das Loch kleben.Jetzt habt ihr eine doppelseitig klebendes Band das absolut abdichtet.Einfach anheben und impfen..sofort wieder zumachen!Ohne Zeitverzögerung.Hält auch Hitze aus.Alternativ anheben etwas rotes Silikon drauf und trocknen lassen nachdem zugemacht wurde.Lässt sich anheben und zumachen!Null Kontaminations! Probiert es aus!I have Baggies ..have no Gummiports.Became this Idea!!!
![Megan Thee Stallion - Neva Play (feat. RM) [Official Video]](http://i.ytimg.com/vi/TpYTyAaTRts/mqdefault.jpg)
The wet paper towel tip made the entire video worth it! (the rest was great too)
😂 I was literally going to say that exact same thing man. I wasn't even going to watch this video because I already know this technique but after I seen the wet paper towel with the silicone I said well damn I guess my theory is right that you can learn something from everybody.
Kudos, my friend. Thank you for making a video for all of us ignorant mushroom lovers😂😊.
Dude thanks for this. By far the simplest most cost effective method I’ve found
using a wet rag to fix up the port is f***en INSPIRED! Thank you dude!!
Glad I could help! Thanks for watching 🙏
Love your paper punch hack for the holes.
I've been drilling them and the burr can be a pain to clean up.
If you push lightly from the inside with the wet paper towel on your very last massage of the RTV blob you will get a nice raised convex area exactly above the whole.
Like a Bull's Eye for the syringe needle.
My hole puncher broke I've been using a hammer and a nail its so much faster
I love that idea!
Gracias senior, by far the simplest and makes the most sense of any method I've seen.
Thank you! You saved me so much money. Terrific and helpful video
Good explanation and clear instructions!!!!
Thank you so much 🙏
@@SporenSprout Is it necessary for me to make a hole for the gas exchange? I mean can I get the same result by loosening the lid after sterilization?
Sir, I appreciate your videos.
May I suggest that you use the new cheap, thin Chinese snap lids for this purpose, as they are So thin as to be questionably functional for canning purposes. They will be much easier to punch.
Excellent video! This is RipperJack FYI, the person who sent you a message regarding an order last night.
Great tips, concise video. Thank you!
I had been looking for this video so much jajaja Thank you!!!
Thanks for watching!
God bless you for this
I really appreciate your comment 🙏
Very helpful, thanks!
Very helpful
Great video, thank you!
Will give the one layer a try this go thanks ☺️
You make this really easy.
Useful, thank you
Amazing 😂💯📈
Thank you so much!
thanks
Loving the tattoo, very cool
Broke AF and this is making my day. Hey man, why Red silicone?
Is there a specific reason you use a hole punch to make the holes or can the same results be achieved with a drill or a hammer and nail or something else?
You can definitely use whatever works best for you.
Wont the tape adhesive wane if it's pressurecooked?
No it’s paper tape
Just made mine last night weoooooo!!
Long shot on getting an answer since this is such an old video but can these lids be used in a pressure cooker to sterilize grain?
Yes I use them for grain and LC media just fine.
@@SporenSprout❤ thank you so much! I decided to order the injection ports but I was mostly concerned about the mocropore tape but would that then eliminate the need to unscrew the lids when under pressure?
@@Klinezilla I still unscrew the lids a bit while using the 2 layers of micropore tape and pressure cooking but yeah it’s not really needed for grain jars. With LC if you don’t unscrew a bit the liquids will splash up and hit the get the tape wet under pressure. Wet tape can cause contamination.
@@SporenSproutthank you so much 🎉❤
i was about to say that is the sole purpose of using foil in both a PC and or instapot along with the broke boi -popcorn tek boil method ( i dont use this) i prefer the instapot have had good results so far@@SporenSprout
Question: Would you be able to just have one hole for injecting and then cover it with micropore tape after innoculation?
Yes thats fine
@@SporenSprout ig the paper tape isnt bothered during pressure cooking or steam bathing ur jars? Meaning the adhesive doesnt lose any hold or degrade during all that heat?
@@NightWitch1630 No, 2 layers holds up perfect.
@@SporenSprout lol okay I can do that 😝
@@NightWitch1630 two layers of tape and some aluminum foil over top of the jars (protects from steam seeping through tape) while pressure cooking and you're golden.
Thank you sir👍🏼
Could u also use micropore tape for the gas exchange tape … or should u use the paper tape
Yes micropore tape is best. Check out these new lids tho! ruclips.net/video/naQryLe8SHk/видео.html
is the paper tape enough? don't you need micropore tape? or are they pretty much the same?
They are the same. Medical paper tape
I've mostly seen the air exchange hole filled with polyfill. Is paper tape alright too? On shroomery a few users were saying micropore tape here is much worse than polyfill.
I’ve made a very large number of liquid cultures like this and I’ve never had a problem. 2 pieces of 3M tape works everytime. Just make sure to keep the jar high off the ground. You don’t want a bunch of dust settling on the lid. You can wrap a coffee filter around the lid for extra dust protection if your room is dusty. The cool part about this hobby is that you can do whatever works for you, so feel free to experiment with whatever is easiest for you to access.
Does paper tape prevent harmfull bacteria from getting inside the jar? 🤔
Yeah I would use 2-4 pieces. It does prevent contamination especially if you can keep it from getting wet and prevent dust from landing on the lid over time. If you want more security you should use waterproof synthetic filter discs instead.
does the micropore tape withstand sterilization? is it better to replace it after sterilization to make sure it has fresh good glue?
It does withstand pressure cooking and doesn’t need to be added after
Where'd you get the background music from its 🔥
Do I put the tape on before it goes into my PC
Yes 2 layers
Perfect. I have plenty of tape but don't wanna go out and get polyfill. Do you suggest tinfoil over lids while pressure cooking?
@@sethhaze1610 Yea I use foil
Why red RTV and not gray. I’ve seen both but wasn’t sure if it was temp related. I’ve got a few tube of the gray that are unopened and all ready paid for by some other project
Grey RTV has higher density when cured for higher vibration resistance and tight tolerance applications. Red RTV is for exhaust and high heat applications. Gray will probably work fine but I have not used it.
Genius, pre-made lids are like 2$ each. only thing I don't already have on hand is the silicone
If you wanted to get crazy, you can just inoculate through a hole covered with micropore tape and then just put another layer of tape over the hole very very fast and in a clean environment.
@@SporenSprout sounds like a good idea for grains but I might as well invest in the silicone for liquid culture
Just wondering if this will work on plastic ball lids.?
Thanks great information 👍
Yes it works
Thanks mate.
Do these hold up pretty good after being used in a pressure cooker a few times?
Yes. I have had no problems.
Hey thanks so much how long till they dry enough to go in pressure cooker.
Do they hold up. Thanks you saved me 100 bucks if not 300 over time
They take 2-3 hours to dry but as soon as the Rtv is not sticky anymore you can use them and the pressure cooker will finish solidifying the Rtv. They hold up very well, and you can add more than one injection port if you want in the future.
@Spore n' Sprout thanks man so much this is a game changer !!
Do you have a link to which exact jars you are using? Thanks in advance!
hey, will the paper tape "survive" the pressure cooker?
Yes
Does black etc work too?
My surface is perfectly clean (cleaned with acetone) but the silicone peels right off after drying... this is useless. Going back to self adhesive injection ports... much easier to apply and stays on.
I have made thousands of them and learned how to do it in a way where it doesn’t peel off. It’s just how you did it.
@@SporenSprout How? From what I read, silicone doens't stick to metal or plastic. I have tried, sanding the surface, clean the surface etc. It still peels off just by pickig it with finger nail.
I don't have a hole punch so what size drill bit would you recommend? Or does it matter?
1/4 “ is good. Doesn’t matter much as long as you can fit the needle though.
How many uses can you get out of each lid?
About 5 times before disposing.
Once the injection ports are spent or done…is that easy to remove to make a new injection port? Thanks!
Yes, you can tear it right out.
Do you by any chance know if the paper tape is going to survive the pressure cooker pasteurization?
Yeah it does. Use 2-3 layers
@@SporenSprout ay appreciate it bro, still answering comments on a 3 year old video
@@davisen3257 No problem! Good luck man! 🍄
After flame sterilizing do you stick the red hit needle into the silicone or wait for it to cool?
Wait for it to cool.
Can the paper tape tolerate being pressure cooked?!
Yes, 2 layers is best.
@@SporenSprout thanks!
Hey spore! My spots of silicone are no longer sticky but they still smell really strongly; will the grains be safe? I put the lids on the jars to carry through to another and when I took them off it smelt really really strong. Just wanted to check before putting my grains in the pc!
When I squeeze out the silicone, the lid is set on top of a lid ring and that’s where it cures for around 4 hours and then can be pressure cooked. If you mean that you let the silicone cure while set on top of a jar, the bottom silicone probably is still wet because no air got to it. The smell of silicone isn’t a big deal so I wouldn’t worry about it.
@@SporenSprout yeah that’s exactly what I did! I’ve left them now to cure overnight probably. Thanks 🙏
Do u shake and break with this method? Doing my first inoculation tomorrow and I read somewhere that if u shake the jars, if any of the grains touch the microtape it could potentially cause contamination. Let me know what ya think!
Yes shaking is perfectly fine. Don’t worry about that, It will not happen.
@@SporenSprout great! thank you!!
@@samanthamolencamp6017 spray some iso onto the tape if you are concerned about that happening. Likely to have any effect but you cannot be to sterile
Is it ok to use micropore tape instead of paper tape?
Yeah. Check this out though: How to Make the Best Mushroom Liquid Culture Lids
ruclips.net/video/naQryLe8SHk/видео.html
@@SporenSprout Thank you! I ordered your waterproof filter disks from your Etsy store! 🙂
Happy to support your store! Thanks for such a great explanation.
I'm enjoying watching all your vids. Peace and thanks!
Can you use jars you find, or do they need to be mason jars?
Any jar is good
What kind of a tape is it?
Here: amzn.to/4bTZzQ5
Or check newest video: How to Make the Best Mushroom Liquid Culture Lids
ruclips.net/video/naQryLe8SHk/видео.html
A peice of advice use parchment paper to smooth it out
Thank you. Is this micropore tape good for liquid culture?
I have used 2 layers of tape for liquid culture for years and it works great. Just try not to splash the liquid up and hit the tape.
@@SporenSprout Thank you, you are great!
Is it ready to go after this procedure? Does any of the silicone go inside the hole of the syringe ? Thanks!
After the silicon drys it’s ready to go. Sometimes a tiny piece of silicon will get stuck in the needle when injecting and end up inside your liquid culture. If you insert the needle at an angle with the opening of the needle end facing up and then straighten normally, it works better for me. You shouldn’t have a problem with the tiny red piece of silicon causing contamination.
@@SporenSprout Thank you for that. I have some small silicon that ended up in my jars and agar jars and hoping for no contamination from it.
Am I still good to shake my grain jars when they're partially colonized? And do you keep tin foil on through the colonization process or just until they're inoculated?
Yes you can shake the jars at around 25% to speed up colonization. Tinfoil is not used during colonization.
Thank you for being so responsive; I have my lids cureing now!
@@sethhaze1610 no problem! The silicone will usually withstand around 6-10 injections before becoming compromised. It’s easy to replace the silicone or dispose of the lid entirely if it starts to rust. I’ve been using these lids religiously for years and I love them, good luck!
When we inject spores can we use a lighter to sterilize without damaging the inoculation port or do we just use iso alcohol?
Yes you can use a lighter without causing damage. 18 gauge needle works well, I wouldn’t go any thicker than that. If the needle is brand new out of the sterile package I only use ISO
@@SporenSprout How about forming the silicone into a little bowl that would hold a couple drops of alcohol?
@@zhuliu There is no need really, just wipe the silicone port with alcohol before injection and make sure to use a sterile needle.
Have you ever tried just injecting through the paper tape and immediately covering the injection sight with another piece of tape? This seems easier in my novice rookie brain.
Yes I have done that, it was actually my first video:
ruclips.net/video/vQMiybw67ak/видео.html
Hey where do you get that tape, , it breathes ? What’ is it called .It doesn’t come off in the pressure cooker.
amzn.to/3eIngCK
Do 2 pieces of the tape. Works every time.
will this work for liquid culture lids
Yeah I use them, just don’t tip the jar upside down and get liquid on the tape
Any reason you use red RTV? I have black, and clear. Does red stand up to heat better?
Red is high temp resistant
@@SporenSprout Thanks, I googled the brand I have and it says high temp 650 degrees. Guess the brand matters.
So if I am using these on broke boi technique/jars, should my lids be fully closed shut when streaming them in a pot or slightly cracked open?
Tighten the lid ring and then loosen it a quarter turn. Sometimes I forget to loosen it a bit and it’s not really a big deal. The micropore tape might come loose a little so just make sure the tape is secure after.
@@SporenSprout thanks! Then I'm sure you close it up after once it's cooled down right? Since the tape hole provides CO2 exchange?
@@wyattzelenka1 Yes I tighten it immediately after it’s done.
@@SporenSprout you rock thanks so much!
I did this but my micropore tape got wet from the PC even if I wrapped it in foil. Is it still good? Or will it likely contam the jar?
That happens to me a lot and never causes problems but I do try to avoid that. It’s more of a concern if after months of sitting, the liquid splashes up and hits the tape.
@@SporenSprout Ok thank you! I was thinking that once my LC is ready I'll transfer in 10ml syringes and store them in the fridge like that, is it better than keeping it in the jar?
@@Fourpey Yes that is a good idea.
How many times can these ports be injected before starting to lose their integrity?
@@aarondavis8224 usually around 10 injections or so
Hey ,can i uee any multi purpose sealent
Thanks
Has to be high temp
@SporenSprout Cheers bro 👌🏻
Is this fine for an autoclave?
Yes
@@SporenSprout thx a lot
How does the paper tapes holds up after the pressure cooker ?
Perfectly fine
Is paper tape the same as micropore tape?
@@tamie341 yes.
remember your aluminum foil over the lid during cook and it holds up perfectly.
So the paper tape holds in 90sec pressure cooker?
Aluminum foil over the lids during cook. Keeps the steam off of the lids. Your tape will be fine.
Question: a dude from reddit said that micropore tape should be placed after pressure cooking. True?
No, micropore is used before pressure cooking.
@@SporenSprout thank you for sharing this. I am trying my first grow using this method. do you use foil as well in the pressure cooker along with the tape?
@@chrixin1 It works every time! Yes, cover the lid with a square of tinfoil to prevent moisture from seeping in through the filter.
@@SporenSprout do they seal when u pc them?
Micropore tape before cooking, remember your aluminum foil. Protects the lid from moisture. It doesn't hurt to do two layers of tape. I always do, make a little x.
Will the tape and silicon weaken when jars are in a pressure cooker?
No they are fine.
i have the same question! thanks for asking that.
Is that a psilocybin tattoo?
It’s serotonin
demonstration?
Lots of demonstrations in my other videos
I don’t understand the point of having a sealed injection port if there is a paper tape vent where air can freely move in and out ? Not being a dick. I honestly don’t understand ?
Yes paper tape blocks contaminants from entering while allowing fresh air exchange
@@SporenSprout ohhh ok. That makes sense then. Air flow but no bacteria contamination. Gotcha. Thanks. Great vid
Technically you don’t need the silicone and could just use paper tape for both , am I right?
@@billybob1052 yes
@@SporenSprout does 3M fabric micropore tape not work as well as paper?
The simplest Methode: (German...dolmetsch this!)Kauft Teppichklebeband.Schneidet so tief ein daß ihr 2Lagen runterschneidet.Auf das Loch kleben.Jetzt habt ihr eine doppelseitig klebendes Band das absolut abdichtet.Einfach anheben und impfen..sofort wieder zumachen!Ohne Zeitverzögerung.Hält auch Hitze aus.Alternativ anheben etwas rotes Silikon drauf und trocknen lassen nachdem zugemacht wurde.Lässt sich anheben und zumachen!Null Kontaminations! Probiert es aus!I have Baggies ..have no Gummiports.Became this Idea!!!
Thanks