The use of 2 𝐻 2H in the Signal-to-Noise (S/N) ratio calculation is primarily due to the main signal consideration, not the noise signal. Main Signal Consideration: The signal (the analyte peak) is expected to be symmetric around its apex. When you measure the height of the peak ( 𝐻 H), it represents the intensity of the analyte at the apex. However, the total signal contribution includes both the rising and falling portions of the peak. Thus, to properly account for the total signal strength, we use 2 𝐻 2H, which reflects both sides of the peak's vertical span, ensuring an accurate estimate of the analyte's signal.
Sir same doubt here, for example if Run time is 50 minutes and peak eluted 20 minutes.than what is criterea of (h) value .near peak OR take up to 50 minutes .
It should be over a distance>or = to 5 times width at half height of main peak Ex. Main peak height is 50 cm so at 25 cm measure width, suppose it comes 2cm so you take > or = to 10cm on both side of main peak
Sir want to know during integration of LOQ solution peak in empower what should be start time and end time for baseline noise . Plaese must reply and give me your contact no. I want to talk to you
The use of
2
𝐻
2H in the Signal-to-Noise (S/N) ratio calculation is primarily due to the main signal consideration, not the noise signal.
Main Signal Consideration:
The signal (the analyte peak) is expected to be symmetric around its apex. When you measure the height of the peak (
𝐻
H), it represents the intensity of the analyte at the apex.
However, the total signal contribution includes both the rising and falling portions of the peak. Thus, to properly account for the total signal strength, we use
2
𝐻
2H, which reflects both sides of the peak's vertical span, ensuring an accurate estimate of the analyte's signal.
Excellent explanation chacha
Very good and Exact explained, thanks sir
Nice information sir
Thanks sir for sharing your valuable information
Thanks for clearity
Excellent sir ..
your videos so useful sir, thanks lot
Great Sir
Very nice and clear but in some videos the background music is quite irritating, my suggestion would be to remove them.
Thank u sir this video is very useful
Amazing sir
I agree with your disclaimer 😂
How do you get H in the first place? Is that from the average value of h?
It's mean u have to indicate capital -H2&H2 instead of for noise base line (h2&h2)
Sir download the mechanism for measuring the quantum yield of carbon dots using quinine sulfate as standard please
Why validation is required pls explain sir
Sir, i have a doubt about the limits of LOQ and LOD limits
i.e., LOQ is 10:1
LOD is 3:1
why we should consider these limits why not the other?
Please sir give one lecture on how to select dissolution parameters and dissolution media
Can you please repeat why the 2 factor is there?????
What's the limit of S/N ratio
Is it method 1 or method 2 sir?
Sir please take classes on stability study's
Sir same doubt here, for example if Run time is 50 minutes and peak eluted 20 minutes.than what is criterea of (h) value .near peak OR take up to 50 minutes .
It should be over a distance>or = to 5 times width at half height of main peak
Ex. Main peak height is 50 cm so at 25 cm measure width, suppose it comes 2cm so you take > or = to 10cm on both side of main peak
please explain how LOD= 3 x [C]/(S/N), LOQ= 10 x [C]/(S/N)
Sir, from which reference book I can learn all this technical concept apart USP?
Snyder book for method deve6
Limit of detection s n ratio
👍👍
One example
Why calculated s/n
Sir want to know during integration of LOQ solution peak in empower what should be start time and end time for baseline noise . Plaese must reply and give me your contact no. I want to talk to you
Hplc calibration