When I find your videos, without fail, you are the one I can trust to fill those tiny gaps of detail to help my understanding. There are many other great efforts on RUclips like this but your videos have added clarity in the drawings and in the voice/ quality. I'm half deaf and I use subtitles which don't even work on most of these highly technical biology videos. I don't even need subtitles with your voice, I can really hear the high frequency consonants which are what piece words together for my deafness type. Thank you for caring and sharing x
Well I sure am glad you didn't scrap this video, even with the mistake (which I realize was a consequence of explaining PCR at the same time, which *would* use two primers for the exponential growth), it was perfectly clear and understandable. Thank you so much! :D
Can you not have fragments, increasing in length, with termination nucleotides farther upstream of normal dNTPs proceeding the primer (and thus in between the primer and termination nucleotide)? I think I may be missing something, but in other words, how does one make sure the nucleotides in between the termination sites are being covered? *My best guess is that the presence of the other ddNTPs along with the other fragments in the other tubes are supposed to obviate the areas not covered by another tube and in effect, “pick up the slack” between tubes to provide full coverage. This question is specifically for the first way.
Thank you so much, Sir! Your explanations a very helpful! How do you make sure that the dideoxynucteotides attach at every possible location? Just by putting enough of them in the tube and allowing enough time?
I don't get one thing, if the dna strand is unknown and we want to sequence it, how do we make a primer for it? How do we know what sequence the primer has to be if we don't know its sequence! Can someone explain
I have one question, I don't get it, how are the bands visualised in the electrophoresis (in the Sanger Method)? Are ddNTP or dNTP labeled some how? Thank you! Great video :)
Don't you need single strand of DNA or just one primer that reacts with one strand of the double stranded DNA, otherwise you are running 2 simultaneous extension reactions? Your diagram shows 2 primers, one for each strand. Your explanation needs some work such as the ratio of ddnucleotide vs normal nucleotide in the chain extension reaction.
Most people on this planet have heard the term "genie in a bottle" ...this term come about in arab lands where the holy trinity under a grand delusion commanded angels to appear coming out of bottles to trick people and telling them they had three wishes that the genie could grant them ...obviously this is more madness from the holy trinity ...over time stories about the wishes that could be granted and became exaggerated ...there is only so much (a little) that the holy trinity can do. They cannot Create anything of a physical form and can only produce visions that have a solid feeling which feel real to people and some think the vision are Creations when they are not. Only The Creator Of All Particles Can Actually Create.
He got a natural human male voice idiot.. This is how we all sound speaking directly to a camera. BTW Your name Robin sounds gay? Are you a male or a female? No clue from your name mate.... Watch out
You cover all the nitty gritty bits that other videos gloss over but that are crucial for comprehension! I finally understand.
When I find your videos, without fail, you are the one I can trust to fill those tiny gaps of detail to help my understanding. There are many other great efforts on RUclips like this but your videos have added clarity in the drawings and in the voice/ quality. I'm half deaf and I use subtitles which don't even work on most of these highly technical biology videos. I don't even need subtitles with your voice, I can really hear the high frequency consonants which are what piece words together for my deafness type. Thank you for caring and sharing x
WOW. I have never taken a biochem course and I was able to grasp what you taught in this video.
That explanation of yours was marvelous! Thank you so much!
Well I sure am glad you didn't scrap this video, even with the mistake (which I realize was a consequence of explaining PCR at the same time, which *would* use two primers for the exponential growth), it was perfectly clear and understandable. Thank you so much! :D
After watching every youtube video on this.. I finally understand it!
Thank you.:)
you're amazing, got an exam tomorrow and this really helped!
Looking at this 2024 and still the simplest out there. Gracias.
Excellent video, I had watched several videos before this one and couldn't quite understand! Thankyou for this in depth video!! 😁
Thank you so much! This has been really confusing for me and you did a great job of answering all the questions I had. :)
oh my gosh this was so easy to follow along and understand. thanks so much!
Thank you for the detailed video! It helped me understand Sanger Method better~
Thank you very much!!! The video is really great and helpful! I even recommended to my friend. Thanks again.
Can you not have fragments, increasing in length, with termination nucleotides farther upstream of normal dNTPs proceeding the primer (and thus in between the primer and termination nucleotide)? I think I may be missing something, but in other words, how does one make sure the nucleotides in between the termination sites are being covered?
*My best guess is that the presence of the other ddNTPs along with the other fragments in the other tubes are supposed to obviate the areas not covered by another tube and in effect, “pick up the slack” between tubes to provide full coverage. This question is specifically for the first way.
Lovely accent. Very educated speaker. Knows his ochem.
Thank you so much, Sir! Your explanations a very helpful! How do you make sure that the dideoxynucteotides attach at every possible location? Just by putting enough of them in the tube and allowing enough time?
Thanks for such a clear explanation!
Thanks for this great clear explanation.
Finally i understood,thank you soo much...
I don't get one thing, if the dna strand is unknown and we want to sequence it, how do we make a primer for it? How do we know what sequence the primer has to be if we don't know its sequence! Can someone explain
Thank you, this was very helpful for me.
I have one question, I don't get it, how are the bands visualised in the electrophoresis (in the Sanger Method)? Are ddNTP or dNTP labeled some how?
Thank you! Great video :)
So for instance in tube a there are ddATPs AND dATPs ???
this really nice video in explanation
I don't understand. How many ddNTPs do you put in one test tube?? And how can the polymerase products have varying lengths in single testtube??
There are different percentages in the mix, and in the case of T for example it´s quantity will be much higher than the normal dNTP.
How do you make the ssDNA in the first place?? And with only the primer present??
How do you isolate ssDNA for the testtubes??
3:14
Why are you keep making this sound 😖
Sir this is wonderful :)
How do you get ssDNA in these tubes to begin with??
Thank You :) Thorough explanation
Excellent ! Thank you!
How do you ensire that it is not just the 2 ssDNAs that reanneal??
Why dont the two strands just reanneal??
Brilliant video...I'm starting to think science is fun.
Do you do a video about high-through put cloning??
Wont the heat from the pcr reaction destroy the dNTPs?
it was great, thank you
the primer is in the 3 prime end not in the 5 prime end
very well explained!
thank you, it was really helpful.
thank you!! It is really helpful
If you dont know the sequence then how can you use proper prime?
+lateef lateef my guess is that they use a primer on a sequence commonly known to be in every strand (i.e. the tata box) but I thought the same thing.
thanx
HardlySimpleFilmsFCX
tqvm! i hvfinal exam coming n this helps alot in undrstanding
very helpful. Thank you
so awesome keep going!
who easy you made it?
awesome explanation, thanks :-)
Don't you need single strand of DNA or just one primer that reacts with one strand of the double stranded DNA, otherwise you are running 2 simultaneous extension reactions? Your diagram shows 2 primers, one for each strand. Your explanation needs some work such as the ratio of ddnucleotide vs normal nucleotide in the chain extension reaction.
brilliant !
awesome thank you so much
thank you so much
thank u
great video helped alot thumbs up! :)
Thank you for this! :-)
Thanks a lot :)
very didactical!
thanks
Le but de cette méthode svp!!
THANK YOUUUUUU!!
helpful!!
Most people on this planet have heard the term "genie in a bottle" ...this term come about in arab lands where the holy trinity under a grand delusion commanded angels to appear coming out of bottles to trick people and telling them they had three wishes that the genie could grant them ...obviously this is more madness from the holy trinity ...over time stories about the wishes that could be granted and became exaggerated ...there is only so much (a little) that the holy trinity can do. They cannot Create anything of a physical form and can only produce visions that have a solid feeling which feel real to people and some think the vision are Creations when they are not. Only The Creator Of All Particles Can Actually Create.
Won't you only be able to make one round of pcr??
And if not, do you somehow make pcr on 5'-overhang products??
im a sanger why do I know so little
"Agarose" put me off! Rest is good.
Chube
crazy gay voice
He got a natural human male voice idiot.. This is how we all sound speaking directly to a camera. BTW Your name Robin sounds gay? Are you a male or a female? No clue from your name mate.... Watch out
im a transdog
dog born in a human body
DID u assume my gender ?!
check your previlege
How do you make the ssDNA in the first place?? And with only the primer present??