Thank you for the excellent explanation. I wanna ask a questions. As like what you said in the video, one of ingredients for sequencing is primer. How if we have the unknown sequence which there's no information about it in database? I mean how can we decide to choose and design the primer for starting the sequencing? Can it be able to use universal primer? Thank you
An unknown DNA template was sequenced using Sanger’s method. The sequence of the primer used was 5’TCGACGGTGGA 3’. Reactions were carried out in 4 tubes each containing dATP, dGTP, dCTP and dTTP, 32P labelled above primer, DNA polymerase but only one ddNTPs in each tube. For example tube1 has ddGTP, tube2 has ddCTP, tube3 has ddATP and tube4 has ddTTP. Tube 1 produced labeled DNA products of 12nt, 15nt, 18nt and 26nt. Tube 2 produced 14nt, 17nt and 23nt, Tube 3 produced 20nt, 22nt, and 24nt and Tube 4 produced 13nt, 16nt, 19nt, 21nt, and 25nt long products. Write the correct nucleotide sequence of unknown DNA template that should include primer sequence? Can you answer this tomorrow is my assignment deadline please help me
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Very clear explanation. Thanks a lot❤️
Thankyou for being my savier ✨🌸
My pleasure
you explained very well bro ❤
Very clear explanation 🤩 THANK YOU SO MUCH 😇
My pleasure
while drawing the agarose gel diagram which way do u start
Great ❤
Please explain maxam Gilbert DNA sequencing
Thank you sooo much to teach indetail bro ur God to me ❤️
My pleasure
EXCELLENT
Nice explaination
Please explain how we got the results..i-e we want to find the sequence..
Thank you for the excellent explanation.
I wanna ask a questions. As like what you said in the video, one of ingredients for sequencing is primer. How if we have the unknown sequence which there's no information about it in database? I mean how can we decide to choose and design the primer for starting the sequencing? Can it be able to use universal primer?
Thank you
Incase of unknown sequence how could you fit primer exactly the terminal of the dna sequence?
sir yae botany or zoology dono same process hai dna sequencing ki
How can sequence of primer be known when don't know about the sequence of DNA?
An unknown DNA template was sequenced using Sanger’s method. The sequence of the primer used was 5’TCGACGGTGGA 3’. Reactions were carried out in 4 tubes each containing dATP, dGTP, dCTP and dTTP, 32P labelled above primer, DNA polymerase but only one ddNTPs in each tube. For example tube1 has ddGTP, tube2 has ddCTP, tube3 has ddATP and tube4 has ddTTP. Tube 1 produced labeled DNA products of 12nt, 15nt, 18nt and 26nt. Tube 2 produced 14nt, 17nt and 23nt, Tube 3 produced 20nt, 22nt, and 24nt and Tube 4 produced 13nt, 16nt, 19nt, 21nt, and 25nt long products. Write the correct nucleotide sequence of unknown DNA template that should include primer sequence?
Can you answer this tomorrow is my assignment deadline please help me
Is it become 5'-TCGACGGTGGAGTCGTCGTATACATC-3' ?
Isn't it polyacralamide gel electrophoresis
Off course ur lecture will be helpful... But a small suggestion pls give slow explanations... There is chance to understand very easily yet..
Play the video on 0.75x speed
This video is well explicit
Thank you
how can you know primer sequence as the sequence to be performed unknown to you
Primer sequences depends on our intrest. To know that exact location only we are using primers of our intrest
@@bioscience6853 for a new microorganism how can u use primer to sequence its genome??
❤
Thanks bro
Thanks sir❤️❤️
My pleasure
Tq sir
My pleasure
Tqqq😂