Thanks Eva. I am learning Cryo EM now and it was really curious when you mentioned about septins in yeast. I was the first person to solve the structures of yeast septins at high resolution. Nice how we are connected by coincidence.
1. Her presentation is top notch. She speaks clearly and intentional, boldly and confident. She's articulate and logical. 2. She's extremely intelligent. 3. I'm very much attracted to her, I can't hide that! Perhaps some day I'll marry a TEM CEM Electron Microscopy professional.
Slightly annoyed by the use of metaphors and personification, rather than explaining some things scientifically, however, it was generally a good video.
One thing caught my attention on the cryo electron microscopy method... You say, fast freezing, a million degrees per second. Really? Think about that and then please define your meaning of the word degrees.
It is not about defining degrees. Your fallacy is probably that you think of the temperature scale and a million degrees and wonder how that fits in. However, the sample goes from ambient to liquid nitrogen temperature, i.e., from around 20 C to -196 C. That is a drop in -216 C. Now if that temperature drop happens in a time frame of a couple of hundreds of nanoseconds, that is a cooling RATE of a million degrees per second, which neither implies that your sample was acquired from the surface of the sun nor that it is cooled to temperatures where even hell freezes, i.e., below absolute zero at -273.15 C, but only that heat exchange happens extremely fast.
This has answered all of the questions I had about cryo-EM! Thank you!
Thanks Eva. I am learning Cryo EM now and it was really curious when you mentioned about septins in yeast. I was the first person to solve the structures of yeast septins at high resolution. Nice how we are connected by coincidence.
5:30 Electron interactions.
25:00 Sample types.
Thank you, Eva! Great introduction! Very helpful!
Thank you! Very clear, detail and very well explained. Very good teacher. Lovely !
very useful..thanks a lot Eva..
Opened my mind into more fascination with EM
Thanks Dr Nogales. It is very helpful.
At about 7 mins in, that is not an X-Ray image. It's a Nulcear Medicine bone scan.
Love her work on tubulin :)
This is amazingly explained.
Thank you Dr.
introduction was really great, helped a lot for studying, thank you
Thanks for posting this video!
Nice and informative. especially for biological samples.Super.
Very well explained. Thank you Eva
Worth wathing this video...! Thnx much for posting it,..!
beautiful video, very helpful. Thank you very much.
great information please provide your references for how you discovered these idea's
When an expert in a field run analysis from sample prep to image rendition!
Really excellent video.
This is such a good lecture video on this topic! Thank you!
thank you so much Eva
thank you very much great explenation!
1. Her presentation is top notch. She speaks clearly and intentional, boldly and confident. She's articulate and logical.
2. She's extremely intelligent.
3. I'm very much attracted to her, I can't hide that! Perhaps some day I'll marry a TEM CEM Electron Microscopy professional.
Amazingly instructive video though.
Eva you Rock!
very thankful
good video and buen acento...
Thank you
WOOHOO, ELECTRON MICROSCOPY!
GO SCIENCE! ROCK ON!
Great... thanks!
Fantástico!
it can only be seen through electron microscope hiv it microscopic
Need EM test to get diagnosed with hiv its microscopic.
necessity mother of invention
Cheers
She is 🔥
for hiv
Very good video gracias. You´re pretty
Slightly annoyed by the use of metaphors and personification, rather than explaining some things scientifically, however, it was generally a good video.
One thing caught my attention on the cryo electron microscopy method... You say, fast freezing, a million degrees per second. Really? Think about that and then please define your meaning of the word degrees.
It is not about defining degrees. Your fallacy is probably that you think of the temperature scale and a million degrees and wonder how that fits in. However, the sample goes from ambient to liquid nitrogen temperature, i.e., from around 20 C to -196 C. That is a drop in -216 C. Now if that temperature drop happens in a time frame of a couple of hundreds of nanoseconds, that is a cooling RATE of a million degrees per second, which neither implies that your sample was acquired from the surface of the sun nor that it is cooled to temperatures where even hell freezes, i.e., below absolute zero at -273.15 C, but only that heat exchange happens extremely fast.
Hot
YEAH! SCIENCE BITCH!
Hot chick doing the science...