Keynote Presentation: Nucleic Acid Detection with CRISPR Diagnostics

Presented At:
Molecular Diagnostics Virtual Event 2019
Presented By:
Omar Abudayyeh, PhD - MIT McGovern Institute Fellow
Speaker Biography:
Omar Abudayyeh is a MIT McGovern Institute Fellow where he conducts independent research on investigating novel bacterial defense systems for genome editing and gene delivery properties. He previously was at Harvard Medical School and the Harvard-MIT Health Sciences and Technology program as an MD/PhD student.
Co-Presented By:
Jonathan Gootenberg, PhD - MIT McGovern Institute Fellow, Massachusetts Institute of Technology
Speaker Biography:
Jonathan Gootenberg earned his bachelor's degree in mathematics and biological engineering at MIT. He received his PhD in Systems Biology from Harvard University, during which he conducted research with Aviv Regev and Feng Zhang at the McGovern Institute and Broad Institute of MIT and Harvard. During his PhD, Gootenberg focused on the development of molecular technologies for treating and sensing disease states, crossing disciplines by utilizing novel computational techniques, microbiology, biochemistry, and molecular biology to uncover new CRISPR tools, including Cas12 and Cas13.
Webinar:
Keynote Presentation: Nucleic Acid Detection with CRISPR Diagnostics
Webinar Abstract:
Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen detection, genotyping, and disease monitoring. We combine the RNA- targeting CRISPR effector Cas13 with isothermal amplification to establish a CRISPR-based diagnostic (CRISPR-Dx), providing rapid DNA or RNA detection with attomolar sensitivity and single-base mismatch specificity. We use this Cas13a-based molecular detection platform, termed SHERLOCK, to detect specific strains of Zika and Dengue virus, distinguish pathogenic bacteria, genotype human DNA, and identify cell-free tumor DNA mutations. By leveraging CRISPR enzymology and lateral flow devices, we demonstrate further improvements on SHERLOCK, including multiplexing and instrument-free readout. CRISPR-Dx forms the basis for multiplexable, portable, rapid, and quantitative detection of nucleic acids.
Learning Objectives:
1. At this seminar, attendees will gain and understanding of CRISPR enzyme diversity and the distinguish characteristics of Cas12 and Cas13 enzymes.
2. After attending this seminar, attendees will be able to describe the development of CRISPR tools for diagnostics in the field and the clinic
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[@emilywangvan]

wow, u r brilliant!