Transformation, Conjugation, Transposition and Transduction
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- Опубликовано: 27 фев 2018
- Bacterial genetics is the study of the genetic material and mechanisms that govern the inheritance, variation, and expression of traits in bacteria. Bacteria are single-celled microorganisms that possess a relatively simple yet highly efficient genetic system. Understanding bacterial genetics is essential not only for deciphering the fundamental principles of genetics but also for applications in fields such as medicine, biotechnology, and environmental science.
Here are some key concepts in bacterial genetics:
1. Chromosomes: Bacterial cells typically have a single, circular chromosome located in the nucleoid region. This chromosome carries the essential genetic information required for the bacterium's survival and growth.
2. Plasmids: Plasmids are small, circular pieces of DNA that can exist independently of the main bacterial chromosome. They often carry non-essential genes that can provide selective advantages to the bacteria, such as antibiotic resistance genes or genes involved in metabolic pathways.
3. Horizontal Gene Transfer: Bacteria have unique mechanisms for transferring genetic material between individual cells. Three main mechanisms of horizontal gene transfer are transformation (uptake of free DNA from the environment), transduction (transfer of DNA through bacteriophages), and conjugation (direct transfer of DNA between bacterial cells through a pilus).
4. Mutation: Bacteria can acquire genetic variation through mutations, which are changes in their DNA sequence. Mutations can occur spontaneously during DNA replication or can be induced by external factors like radiation, chemicals, or certain drugs.
5. Gene Expression: Bacteria tightly regulate the expression of their genes to respond to environmental cues and ensure efficient resource utilization. This involves processes such as transcription (copying DNA into RNA) and translation (using RNA to synthesize proteins).
6. Regulatory Elements: Bacterial genomes contain regions known as promoters and operators, which control gene expression by interacting with regulatory proteins. The lac operon in Escherichia coli is a classic example of a bacterial regulatory system.
7. Antibiotic Resistance: Bacterial genetics plays a crucial role in the development and spread of antibiotic resistance. Bacteria can acquire resistance genes through horizontal gene transfer, allowing them to survive exposure to antibiotics that would normally kill susceptible bacteria.
8. Genetic Engineering: Bacterial genetics has revolutionized biotechnology by enabling the manipulation of bacterial genomes for various purposes. Recombinant DNA technology involves inserting specific genes into bacterial cells to produce valuable proteins, such as insulin or enzymes used in industrial processes.
9. Genome Sequencing: The advent of DNA sequencing technology has facilitated the complete sequencing of bacterial genomes. This has led to insights into bacterial evolution, taxonomy, and the identification of potential drug targets.
10. Evolution and Adaptation: Bacteria have incredibly short generation times and high mutation rates, which contribute to their rapid evolution and adaptation to changing environments. This evolutionary process has resulted in the diverse array of bacterial species we observe today.
Bacterial genetics continues to be a vibrant field of research, with ongoing discoveries shedding light on the intricate mechanisms that govern bacterial traits, interactions, and evolution.
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You took a 2 hr lecture from my instructor and made it understandable in a little over 3 min. Awesome!
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Man, these processes seemed so complicated when reading my textbook. Thank you for breaking it down!
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TransPosition: plasmid creation or takeup. TransFormation: Fresh DNA in pool enters bacteria. Transduction: virus shares with bacteria. Conjugation: F+ have plasmid and pili that share with F- bacteria.
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Please explain in detail of transduction
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And thanks a ton for watching
@@USMLEpass can you make a video about Hardy Weinberg population genetics? Please.
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@2:30 what about bacillus subtilis? They're competent too.
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Please tell me how f- bacteria forms ?
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Hey there, the IOS version of the App is currently under development... sorry
if bacteria single cell with dna started life then where did the information came from?
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so when a cell dies and the DNA is set free, another cell can pick it up and incorporate it in its own DNA, which is known as transformation, right? but i was wondering, why would the cell do that, why does it need strange DNA? i know scientists can do this too, but, cells can naturally do this on their own, but why would they?
Correct. I don't know why they do that, they just do. Perhaps because they misrecognize the free DNA as a fragment of their own genome and try to "fix" the broken fragment by integrating it back into their chromosome
@@USMLEpass thanks for sharing your thoughts, despite there not being a clear reason (yet)!
That would be an interesting research idea though!
I know, I know, I should be focusing on the lesson at hand, but is that a Stardew Valley track playing in the background??
FOCUS!!
By conjugation only f+ bacteria forms ? Please give me rpy
Only F+ bacteria can form the mating bridge to start the conjugation process
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please provide references : )
They're in the description under "Refrences"
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All these videos on RUclips about this. They are so sure this happens. So, where's the video evidence from a microscope showing this? After all, if they KNOW it happens, they must have observed it, right?
Uh, yes. You can watch it happen yourself if you like. It doesn't take that high of a magnification to see this stuff. There are real micrograph photos in your textbook. At least there are in mine. What's your angle here?
You could also just Google "bacteria conjugation real video" before you get into conspiracy theories.
Olunga pesuda1st..ipdi explain panranra perula theeya karachi kaadhula oothaadha..
Thanks