Negative staining of particulate samples for TEM

-A guided walk-through of the practical steps of negative staining of particulate samples.
-Negative staining is a rapid method for screening the morphology, size and arrangement of particulate samples such as proteins, vesicles, isolated organelles, whole viruses as well as bacterial flagellae and pili. The technique can be combined with immunogold detection.
-Find the protocol for negative staining here:
www.gu.se/site...
-More info about negative staining at CCI Gothenburg:
www.gu.se/en/c...

[@lukeaddington2803]

Great video, thank you! Which forceps and auto-pipette are you using?

[@cciuniversityofgothenburg4145]

Hi Luke,
I am glad that you liked the video, and thank you for commenting!
In this video, Anna is using 3C biological grade forceps (503C-NM) from Dumont, which you can order for example on this link: www.tedpella.com/twzr-mis_html/twzr-bio.htm . As long as the forceps are biological grade, we recommend our users to decide which kind (non-inverted, inverted, curved, etc.) according to their own preference.
Regarding the pipettes, we use the this kit in the video www.fishersci.com/shop/products/fisherbrand-elite-pipetter-kit/14388100 .
Best regards,
Charlotte Hamngren Blomqvist on behalf of the CCI team

[@lukeaddington2803]

@@cciuniversityofgothenburg4145 Thank you very much!