Sample Preparation for Electron Microscopy

Medicine Technology - School of Medicine - JU
Dr. Hanan Jaafar

[@rabeaaslam3968]

Fixation by glutaraldehdye
Wash with optimum tetraoxide
Wash with pbs
Dehydration with ethanol
Propylene oxide
Infiltrate with resin
Embedding put resin inside a mold
Oven for overnight 60 degrees
Cut the plastic mold
Sectioning by ultramicrotome

[@wafaamaged3570]

Good video

[@alannolan3514]

You deserve a raise!

[@srikantadash4603]

😘😌o god 🙏.... Awesome yar... It's just amazing.

[@ankitayadav1123]

Thank you Ma'am for this clear explanation 😀

[@tariqkhanPathanKabuliwala]

Excellent, very complicated. Thanks for sharing, enlightening us.

[@simranshah3478]

Wow!! Amazed. Thank you

[@karenbernaba6793]

wow you saveddddd my life! best video

[@rudraprasad8934]

Very well explained
Thank you for your hardwork

[@minalbhalerao]

Very useful video of ultramicrotomy sample preparation and processing. Thank you very much.

It's more complicated than I thought :D Thank you.

[@Jozn3]

Some tips at sectioning. Remove the spec. trans light cable. Turn the Chuck not the hole Seg.Arc. and use a Trimmer with a diamond cutter. The razor blades causes defects in your diatome knife sooner or later. :-)

[@uroosamanal8356]

Thank you mam🌺
Very Interesting and Amazing ☺👍

[@ahmadsalim1569]

Thank you so much it was really helpful. Keep up the good work

[@Manisha_Gusain99]

Very much intresting 👏👏👏

[@treefafaroqismail6222]

thank you for sharing very clear and interesting explanation

[@dertejavi1]

hi i have a question, first at all dorry for my english!. to discard the osmiun u use a container onli for it? or u mix with the another discards? thx

[@exploringworldbeauty]

how long sample need to put osmium tetraoxide ? after stain with lead citrate then the sample can be taken for electron microscope ?

[@aliya9711]

how much time the sample should be incubated with Osmium Tetroxide

[@UmaMaheswari-jq4dq]

Thanks a lot

[@as91as1]

very helpful thank you so much

[@mistikaalmira7174]

How do you prepare red blood cells for SEM?

[@factsfirm6672]

Why do we add uranyl acetate first and then lead citrate?

[@samahalhorani5566]

Can be applied for TiO2 sample preparation

[@krishnappa3982]

What is that drop in which you are finally dropping the grid?

[@galactusgamerzzz2104]

Thank you madam

[@sabafj369]

Some of the steps the voice was cut. How much time needed to keep the sample with the stain? Same thing with the last step.

[@alejandratellez8848]

“We are now sectioning our sample *machine beeps* oh god dang it” I felt that

[@ayeshairshad8681]

can you tell me what was the specimen used in this prepration

[@alejandratellez8848]

@@ayeshairshad8681 no, they never mentioned what sample it is but my guess is tissue but looks more like plant

[@ayeshairshad8681]

@@alejandratellez8848 ok thanks

[@anjusangwan4217]

can you tell me which resin to use for post embedding of plant samples? mention resin name please

[@krishnappa3982]

10:03 What is name of blue color container having water and knife in it?

[@paramedical8133]

60 degree temp incubation????

[@amiraanwar6095]

thanks

[@anjusangwan4217]

What was the resin name? please mention

[@uroosamanal8356]

Epoxy Resin....

[@megraelynsatterthwaite]

do you really think this research reflects biological reality after all this processing?

[@II-th7bn]

Nice question

[@ezinneimoko1898]

Please what is the first staining reagent?? I heard urine acetate 🤔

[@fasfas5405]

Uranyl acetate

[@archanamohandoss9975]

😂