Learn About Illumina's Infinium Protocol

Let's walk through the protocol for the Illumina Ininium assay lab step-by-step.This workflow applies to the Illumina Infinium™ assay lab for HD, HTS, and LCG format BeadChips™ and is intended as a visual introduction and reference for new Infinium users.
Protocol steps demonstrated:
- DNA Amplification, Fragmentation, Precipitation, Resuspension, BeadChip™ Hybridization using a hyberdization buffer, Washing and XStain, and Scanning.
-Key techniques and best practices highlighted:
- Decanting the amplification plate
- Removing the cover seal
- Maintaining the Glass Back Plates
- Assembling the flow through chamber.
See the Infinium Assay Lab Setup and Procedures Guide here: support.illumina.com//downloa...
To learn more about selecting BeadChips, required equipment, setting up your lab, and data analysis, watch the “Discover the Flexible, Scalable Infinium Workflow” here:
• Discover the Flexible,...
#illumina #wholegenomesequencing #infinium #genomics

[@HewanDemissie]

Thank you. This is excellent. Thus, I can work in the lab on my sample.

[@Igcaet]

OMG, this video is perfect! I've searched it for so long! Thanks for uploading, even if it was only in dec 20th, better than never xD

[@Igcaet]

I have a question: if we have a CG heterozygous (like I do have in my data), how will the scan differentiate both green signals and not misinterpret it as being homozygous?
The illumina reference guide itself does now cite "fluorophore", not even once. And both there and in their videos, they only show biotin associated with C and G, which after will have a green signal, and DNP with A and T, with a red signal. How is it possible to differentiate CG or AT heterozygous?

[@yohanaalemseged6683]

thank you for this very intersting !!!

[@user-qi9rr8mk5j]

Hello! Im glad to see this video! And i need a little help. What is the maximum time period between chip dying and scanning? Can i dye chip in my laboratory and then read in second laboratory the next day? Thank you!

[@Igcaet]

Helo! Here with my colleagues we used the Infinium Multi-Ethnic Global-8 Array, and it took us three days the whole process. In the third day we've stained our chip, then after washing it we've put on a vacuum incubator and after 1-2 hours it was completely dry, so we could scan it, with excellent call rates. Watch out for the chip must be read until 72h after staining.

[@user-qi9rr8mk5j]

@@IgcaetThank you for answer!