A good graphical presentation, but some facts to correct. It is stated that, at first, the proteins are partially separated by gravity. This doesn't really happen, because the column is at 100+ bar pressure (where gravity would be negligible), and many times it is in a horizontal position. Also, you show them separating through the column as with reverse phase. This does not happen. They attach to the column once and elute when the salt content is such that the attraction to the column is lessened. There is only one interaction...attach to the column and then elution.
Thank you for pointing this out! I had misunderstood that part of the process. I'll most likely redo my chromatography series in the future to improve these videos and I'll make sure to include this information then!
Please leave feedback! I'm always trying to improve😇
the best explanation, thanks a lot!!! My biopharma professor was not clear enough, you made it simple!
Fantastic to hear!😇
I loved the explanation! Thank you very much, I finally understand this technique.
I'm very happy to hear that! Glad I could help!
This video was incredibly helpful!
I'm so happy to hear that!😇 Thank you!
first subscriber
Good video , easy to understand
Super to hear that!
Simple and easy 👍
I'm so glad to hear that! Clarity, and simplicity is what I strive for!🙏
Thank you!
I'm happy I could be of service!
A good graphical presentation, but some facts to correct. It is stated that, at first, the proteins are partially separated by gravity. This doesn't really happen, because the column is at 100+ bar pressure (where gravity would be negligible), and many times it is in a horizontal position. Also, you show them separating through the column as with reverse phase. This does not happen. They attach to the column once and elute when the salt content is such that the attraction to the column is lessened. There is only one interaction...attach to the column and then elution.
Thank you for pointing this out! I had misunderstood that part of the process.
I'll most likely redo my chromatography series in the future to improve these videos and I'll make sure to include this information then!
@@LucasLearnz I'm just happy that you're out there making this type of content... especially something like the HIC technique
Thanks
Glad you liked it!