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If we have inserted the gene of interest in tetracycline gene then the bacteria will not grow upon treatment with tetracycline then how can we collect it if it dies ????
No, often bacteria with resistance plasmid is resistant to some antibiotics. We differentiate by replica plating. Only bacteria which is transformed with vector can live in ampicillin or tetracycline containing medium. Thank you
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Hi Akash
Thank you so much and welcome!
Glad to share our free certificate course
⏬ Download free E Book: Introduction to Recombinant DNA Technology www.biologyexams4u.com/2022/09/enroll-now-new-online-course-on.html
📢Our Free Online Certificate course on Introduction to Recombinant DNA Technology is live on alison.com; world’s largest free certificate course provider. Enroll now! alison.com/course/introduction-to-recombinant-dna-technology?
Thank you so much:) @biologyexams4u
Nice explanation
Very helpful
If we have inserted the gene of interest in tetracycline gene then the bacteria will not grow upon treatment with tetracycline then how can we collect it if it dies ????
replica plating is for that !!
Tysm for the explanation!!
Happy to help!
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Dont all the bacteries have antibiotik resistance genes how do we differentiate the ones that are inserted or not
No, often bacteria with resistance plasmid is resistant to some antibiotics. We differentiate by replica plating. Only bacteria which is transformed with vector can live in ampicillin or tetracycline containing medium. Thank you