Hi Dr. Raki. I work with beneficial fungi of plants, generally considered safe for humans. I am interested in characterising the extracellular extracts with ethyl acetate. How would you suggest to operate to obtain the culture filtrate? Can I work on the bench Or do I have to do this in the safety laminar? To separate the broth, do I have to use the filter sterilisation with syringes (to remove the spores as well), or can I just filtrate with a Whatmann filter paper and think about getting rid just of the mycelia? THANK YOU
Its always advisable to work in laminar though ur sample is safe you can avoid contaminants from outside if your working in LAF. Filtering the spores would give you more exact information about crystals since your going for characterization spores might be a hindrance
Sir.. I have prepared plant extract using methanol and ethanol solvent.. Now I got semi liquid thick viscous extract... So, what diluent should I used to dilute this crude extract since it is not soluble in water.. Some said DMSO is best diluent but it is toxic.. Sir please suggest me what diluent should I used? Thank you sir in advance😊
Yes it will evaporate all.the solvent then you can add known amount and redissolve. It's must if u do. For lower work at UG and PG we don't do so properly.
My doubt is the I use the methanolic solvent to the extraction of plant material but after extraction how to separate the ethanol and my plant extract when I used the your technique that is not sufficient because in the extract ethanol also be present so how I separate
Hi, sir currently i m working on the green synthesis of silver nanoparticles from plant extract can you help me please i will be very thankful to you .🙂🙂
sir I prepared different solvent extract silver nanoparticles... sir please suggest me that how the uv spectra performed of different solvent extract and I need the silver nanoparticle extract solidify or direct liquid solution is used for uv and which solvent used as a blank
Bro I made extract from seeds and it is an oil extract now how can I make its dilute solutions as in ur previous videos I have seen that if we have 1mg extract then we add 1ml solvent then what about oil?
1:10 ratio for extraction. After evaporation add accordingly to make a known concentration. Watch the video on methanolic extract of plants for more info
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It's a commercial knowledge,and u r giving with free of cost
Great person with great explanation
Clearly audible understandable easily to digest
Thank you means a lot :) Just these small words keeps me going 🙏
Well explained, currently I have started working on some plant extracts ,It was really very helpful.
thank you so much anna
bro thank you .. really i like the way u explainl
Very helpful
Sir I have prepared extract so which size of filter paper I will use. There are many sizes so which size you will prefer??
Thank you so much
Hi Dr. Raki. I work with beneficial fungi of plants, generally considered safe for humans. I am interested in characterising the extracellular extracts with ethyl acetate. How would you suggest to operate to obtain the culture filtrate? Can I work on the bench Or do I have to do this in the safety laminar? To separate the broth, do I have to use the filter sterilisation with syringes (to remove the spores as well), or can I just filtrate with a Whatmann filter paper and think about getting rid just of the mycelia? THANK YOU
Its always advisable to work in laminar though ur sample is safe you can avoid contaminants from outside if your working in LAF. Filtering the spores would give you more exact information about crystals since your going for characterization spores might be a hindrance
Sir.. I have prepared plant extract using methanol and ethanol solvent.. Now I got semi liquid thick viscous extract... So, what diluent should I used to dilute this crude extract since it is not soluble in water.. Some said DMSO is best diluent but it is toxic.. Sir please suggest me what diluent should I used? Thank you sir in advance😊
You should use the same solvent , methanol and ethanol only. Since u used these solvents only in the first
It is compulsory,
Mentha extract have to stay 48 hours in room temperature ???
Mentha or methanol? What 48 hours ??
Mentha(Pudina/Mint) extract with solvent methanol.
It keeping 48 hours in room temperature , is that my question.
Yes it will evaporate all.the solvent then you can add known amount and redissolve. It's must if u do. For lower work at UG and PG we don't do so properly.
The pellet is not horizontal with the tube
Is it due to the angle of rotor ?
I want to know how to prepare nanoparticle from plant extract?
👏👏👏👏👏
How to extract Bisphenol A (phytoremediated) from Plant?
My doubt is the I use the methanolic solvent to the extraction of plant material but after extraction how to separate the ethanol and my plant extract when I used the your technique that is not sufficient because in the extract ethanol also be present so how I separate
thank u
Hi, sir currently i m working on the green synthesis of silver nanoparticles from plant extract can you help me please i will be very thankful to you .🙂🙂
sir I prepared different solvent extract silver nanoparticles... sir please suggest me that how the uv spectra performed of different solvent extract and I need the silver nanoparticle extract solidify or direct liquid solution is used for uv and which solvent used as a blank
Mail me rakeshb96@yahoo.com I'll send my silver nanoparticles paper
Pls. How do I calculate concentration?
What the video in my channel for methanolic extract preparation follow that for calculation
Can u please tell me .. that whatman paper can we use from both side ?
Yes
Can i centrifuge volatile oil to separate residues
Yes you can centrifuge them at around 10,000 rpm for 10 min and separate the residues and collect the oil
Bro I made extract from seeds and it is an oil extract now how can I make its dilute solutions as in ur previous videos I have seen that if we have 1mg extract then we add 1ml solvent then what about oil?
You can add organic solvents which are soluble to your oils like chloroform acetone. Check the solubility practically or by reading papers
@@Dr.Rakesh.B Sir I want to ask about calculations... how much solvent should be add...
1:10 ratio for extraction. After evaporation add accordingly to make a known concentration. Watch the video on methanolic extract of plants for more info
@@Dr.Rakesh.B ok thanks
Plz sir make a vdo on how to prepare sample for gc ms analysis...
It is already there in the channel priya. You can follow the same method how we made methanolic extract. Same method to be followed.
@@Dr.Rakesh.B ok sir...thanks a lot
Please do vedio for ftir sample for both extract
procedure for the isolation of single compounds used in TLC Methods
If we dont have rotory evaporator. What will be the alternative.
You can use hot air oven and dry it but the solvent will be evaporated so it can't be re-used
sir i need help to you how i contact to you?
Thank you soo much Sir, I have an questions regarding antimicrobial screening. Can you share your email Sir
Rakeshb96@yahoo.com
Very helpful