A quick and dirty tutorial on how to quantify a gel image using ImageJ.

[@jikunli]

real useful stuff! much better than those non-video tutorials!

[@almosteverything122]

Thanks for the video. Very helpful as i was doing it for the first time.

[@ioeopep]

Hi Kenny, thank you so much for this tutorial! So helpful!!
God bless.
Greetings from New Zealand.

[@emil9599]

As I know, we can also find dna concentration (ug/ul) of a band if we know dna concentration of ladder. But how to find actual mass of certain band. Lets say I loaded 3ul. But then it separated to several bands. How can I find volume in each band?

[@tianjiang8861]

I used to just draw rectangular boxes on the bands and measure the intensity of that. How is that different from this approach? Thank you!

[@samarmostafa5194]

Hundred thanks for your great useful video

[@anthonytan6143]

I love you so much really saved me

[@madisonlotstein212]

Hi what is the benefit of using one big box instead of individual boxes around each blot?

[@selot1]

Thank you for saving so much of my time and energy!!!

[@quantumodality]

Thanks a million. You saved my day! : ) God bless you.

[@surendra2836]

It is really helpful, thank you.

[@gijs9133]

Analysis of SDS-Page has been done by the quick & dirty method (Kenny, 2012).

[@DungNguyen-nr2xb]

Can Image J evaluate the purity level of protein? How can I mesure purity level of protein? Please, help me

[@kasthurirajendran6293]

Thanks for the video

[@epicureansage]

If I Want to calculate fold change how would I do that and then plot graph in excel?

[@MissUche1]

OMG Thank the Research Gods!!!! I totally appreciate this!!!!

[@OpeningBatsman]

Love your desktop at the start, do you have a link for it?

[@kyoung731]

Here you go: i.imgur.com/hdijH.jpg

[@jqx4552]

great tutorial,thanks

[@givtnc]

Thank u very much! You are a life saver!

[@RRR66620]

Awesome man!!

[@jz1110]

very useful, thanks

[@jaazaq1]

Great job! Thanx a lot.

[@YieeHsu]

thank you very much!

[@patrickmina3349]

Where can i download the software

[@kyoung731]

Right here imagej.nih.gov/ij/

[@TheO0o00]

Thank you very much

[@soydandogan7422]

thank you

[@alvarosequeida761]

The % is calculated respect to what?

[@kyoung731]

To whatever you want.

[@yovitaardiyani3447]

In the video, it's shows 10% until 15%.. What is the band of 100% ? And could we calculate percentage by comparing 2 bands ? For example band A 60% and band B 40% ?

[@kyoung731]

I don't believe ImageJ is capable of opening pdf files. Convert your images to a tif file first.

[@Linda-hd5ew]

Thank you so much!