Hi again, yes, I had a chat to Chris after making this video and he said that from memory the perls was done first and the VG obviously needs to be done last to avoid being washed out. It’s an unusual combination.
Can you explain the troubleshooting of schmorl"s techqnique especially we fail to get the lovely Turnbull blue in most cases inspite the same section produced black color in fontana silver Is schmorl"s techqnique affected by the amount of melanin?
Hi Meroo, Schmorl’s relies upon melanin or lipifuscin reducing the ferric chloride applied to ferrous ions which then combined with the potassium ferricyanide to produce Turnbull’s blue (ferrous ferricyanide). Things that can affect staining. 1. Make sure that your ferric chloride solution is of analytical grade since traces of fe2+ ions will bind to the ferricyanide in solution (does your solution appear slightly green?). 2. We find that some melanin turns more of a dark green colour when treated by schmorl’s. This might be due to original intensity of melanin colour type. 3. I haven’t specifically tested this, but traces of aldehyde within the tissue may non-specifically reduce the ferric ions thus increasing background staining. Hope this helps. Send me a picture if need further feedback.
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Intersting lovely combination
espically perl"s / PAS/ vangesion
as I thought this the order isn't it?
Hi again, yes, I had a chat to Chris after making this video and he said that from memory the perls was done first and the VG obviously needs to be done last to avoid being washed out. It’s an unusual combination.
Can you explain the troubleshooting of schmorl"s techqnique especially we fail to get the lovely Turnbull blue in most cases inspite the same section produced black color in fontana silver
Is schmorl"s techqnique affected by the amount of melanin?
Hi Meroo,
Schmorl’s relies upon melanin or lipifuscin reducing the ferric chloride applied to ferrous ions which then combined with the potassium ferricyanide to produce Turnbull’s blue (ferrous ferricyanide).
Things that can affect staining.
1. Make sure that your ferric chloride solution is of analytical grade since traces of fe2+ ions will bind to the ferricyanide in solution (does your solution appear slightly green?).
2. We find that some melanin turns more of a dark green colour when treated by schmorl’s. This might be due to original intensity of melanin colour type.
3. I haven’t specifically tested this, but traces of aldehyde within the tissue may non-specifically reduce the ferric ions thus increasing background staining.
Hope this helps. Send me a picture if need further feedback.