Movats pentachrome

Finally, the long awaiting video for Movat's Pentachrome!
Despite the length and complicated nature of this stain, it is actually quite straight forward once you break it down into its three key steps;
(1) a stain for acidic mucosubstances (Alcian Blue),
(2) an elastin stain, and
(3) a trichrome stain.
The video also examines the requirement for a lengthy treatment with alkaline alcohol following staining with Alcian Blue. This step is often employed in an effort to stabilise Alcian Blue staining, so what happens if you leave it out? The results are interesting!!

[@ChapmanHistology]

Terrific video, Damien. Very informative and really breaking down each step and why you were performing the different steps. Great work!

[@damienharkin]

Thanks Jamie!
Will be taking a break after this one. Always have a lot of fun, but the editing just takes so much time. Am glad to know that at least one person enjoyed it! Hope to post a few more before end of year.

[@mollymurphy6862]

Marking year one as a lab tech in a Dermpath lab, preparing to sit for the exam to be a histotech - Rte 2. These videos really help bring the protocols to life. Especially since I dont see these on a regular basis! Washington State, USA

[@damienharkin]

Thanks Molly, am glad to help and thanks for supporting my channel as a member. Good luck with upcoming exam. Am happy to answer any questions. Bye for now.

[@nonysweet7506]

Thank you so much 🙏🏼

[@damienharkin]

You’re welcome 😊

[@einsteindisguisedasrobinho717]

Today is my One Year Anniversary being a MOHS tech...I was voted least to make it in histology by my classmates........
Watch what I do in my 2nd year.......
Shocking the world!!!!

[@damienharkin]

Happy Anniversary!

[@chrissygarvey2829]

how much time for the 1.5% tartrazine? it wasn't specified!

[@damienharkin]

1 to 2 minutes should be fine.