How to Interpret Gel Electrophoresis Results: Different types of plasmid DNA

When using gel electrophoresis to help with molecular cloning, you may run into a common problem - multiple bands of the same sample. How do you chose which one to cut? Just as different sizes of DNA run at different rates through the gel, different plasmid conformations run differently as well. This video covers the structure of agarose, the different types of plasmid conformations, and how to interpret your gel's results.
Resources
How to Interpret DNA Gel Electrophoresis Results - www.goldbio.com/articles/arti...
Agarose Gel Preparation Protocol - www.goldbio.com/documents/108...
How to Choose a GoldBio DNA Ladder - www.goldbio.com/articles/arti...
Related Products
Agarose LE (Molecular Biology Grade) - www.goldbio.com/product/868/a...
GoldBio DNA Ladders - www.goldbio.com/collection/dn...
DNA Amplification - www.goldbio.com/collection/dn...
Written and Presented by Karen Martin; Edited by Karen Pierce
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Gold Biotechnology, Inc. - www.goldbio.com
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[@graemelaubach3106]

Very helpful video, with great animations! Much appreciated :D

[@imanalansary4538]

Thanks for your explanations ❤

[@vazquezvalemtine]

God sent explanation, my lecutere literally sent us to look this up. Thanks

[@iashsch_]

Super useful👍

[@regina5613]

Super!

[@jacksonmulwa9406]

Exactly what I want

[@jahidhassan7775]

Very nice video ma'am. Ma'am how to know which one is plasmid DNA and which one is chromosomal DNA by seeing in gel electrophoresis?

[@dorfinhorg9458]

If you see only two bands on your gel, how can you tell what conformation that plasmid is in? For example, it could be supercoiled and linear, or it could be linear and nicked circular...any advice here?

[@katelar7576]

I guess you can digest it with restriction enzyme and have a separate well with it, so you could say for sure where linear version is.

[@nadasalah8455]

Thanks alot❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤

[@arindampaul5256]

Mam ethydiumbromide is added in gel or Sample plasmid?

[@Goldbio]

Thanks for your question. Generally, it is added in the gel. About 3 minutes into our video about gel electrophoresis, it talks about waiting for your gel to cool and then adding the ethidium bromide or equivalent: ruclips.net/video/tMyYgEQ2pf4/видео.html
:)