Research topic # identification of novel antimicrobial drugs # AST & MIC with plant extracts

AST # MIC # plant extracts # identification of novel drugs# antimicrobial drugs# novel drugs# research topic # Genes & Genus
Hello everyone,
Good morning.
Here comes my new video and it's all about the identification of novel antimicrobial drugs. I also explained about how we can apply the techniques of Antimicrobial susceptibility testing (AST) & minimum inhibitory concentration (MIC) in research.
you will come to know how to perform MIC & AST with plant extracts to detect the antimicrobial property of a compound by watching this video.
Wishing you all good luck.
Thank you
Dr. Divya kotagiri
[e-mail]

[@sashanealand8315]

Thank you for taking time to explain everything so well as you are writing, this is the best way of teaching no reading ppt, just explaining as you are doing. Thank you in my country we no longer have such good teaching

[@malairx]

superb mam

[@Mokshaandsashank]

Good explanation

[@krishnamohan3246]

Super 👌

[@kamisettimuralikrishna5051]

Nice explanation

[@basantasarkar7475]

Excellent explanation mam..

[@reenaalhrabi1631]

You are great 😊 keep the great job 👏

[@genesgenus4939]

Thank you

[@sharecare9432]

hope to see plant pathology practical protion like agriculture microbiology

[@sharecare9432]

Namaste mam , I am from Nepal...your videos of microbiology related topic is very useful to us

[@genesgenus4939]

Thank you

[@carolmotale1760]

What else can I use other than DMSO to reconstitute the dried plant extract?

[@sheerinnatasha8799]

Hi mam! How to determine the stock concentration of the plant extract after drying? I've used 10gm of tulsi leaves and 250 ml of ethanol.

[@anumkhan3927]

Hello mam .your way of teaching is very good and second thing is that what are the alternative option of rotatory evaporator ?

[@genesgenus4939]

Soxhlet extraction can be used as an alternative.. Thank you

[@ChiaHumbert]

Thank you mum for the great lessons . But you said we will add 1:1 bacteria solution and antibacterial extract source on the wells meaning we don't need to culture the bacteria before testing?

[@genesgenus4939]

We need to pick bacterial colonies growing on agar medium and make a suspension of it adjusting it to the required cfu/ml

[@user-jq6xi3bc5k]

And how clan know what excited substance make an effect??

[@vishmithathsara4541]

Madam can I know if I want to make fruit extract to perform an antibacterial activity, how should I prepare the extract. I don't think that it needs to be dried right? Or just homogenizing using a blender will be enough?

[@genesgenus4939]

Yaa... You can prepare fruit extracts by chopping the fruit into pieces and then blend it to get the extract, concentrate it using a rota evaporator...

[@suresh-yl6ki]

Nice explanation mam.,
But if we add 10g in 100 ml of solvent and what will be the final concentration of plant product after roto evaporation??

[@genesgenus4939]

you need to check the weight of extract after rota evaporation. use that extract to carry out antimicrobial or antioxidant study which ever area you are interested in. from this stock, you need to make final concentration of 1 mg/ml or 500 mg/ml using DMSO

[@suresh-yl6ki]

@@genesgenus4939 thank you for your information

[@ankitalokhande2916]

Hi mam can we use resazurin indicator dye to check presence or absence of bacteria

[@genesgenus4939]

Yes we can use

[@user-jq6xi3bc5k]

I want to asked about how can l experimental anew drug test exactly

[@genesgenus4939]

To check for antimicrobial activity of any test compound, select few pathogenic strains and perform MIC as described in the video... Thank you

[@nageshnilawar3415]

Hello ma’am
Can we use this dye with drug also????

[@genesgenus4939]

Dye is to be added after incubation period to check for presence or absence of microbial growth in wells each having different concentration of drug

[@basantasarkar7475]

I have 100 mg methanol extract stock after rota evaporation ..then how to make different concentration using DMSO(1%).should I make 1% DMSO first?

[@genesgenus4939]

You can prepare 1mg/ml concentration by taking 1 mg of methanol extract added with 1 ml of DMSO..

[@genesgenus4939]

If this concentration doesn't work for antimicrobial study, then you can increase the concentration accordingly... All the best..

[@basantasarkar7475]

@@genesgenus4939 thank you mam.

[@SaifAli-rr9jb]

​@@genesgenus4939 but the concentration of DMSO in this case will be 100% and it is toxic so he can add to 1mg extract 100ML DMSO and 900ML