Part-1How to avoid contamination in Tissue culture/How to operate autoclave for proper sterilization

Watch this video followed by the second part for the proper usage of sterilization techniques to avoid contamination in plant tissue culture and microbiology related works. Do not miss the tips and tricks given in second part of this video:
--Fumigation of lab: • Fumigation of Lab
--Types of Sterilization Instruments and their principles: • Types of Sterilization...
--Plant Tissue culture Laboratory -Instruments and their principles with their applications: • Plant Tissue culture L...
--Microbiology lab and the Instruments used: • Microbiology lab and t...
--Life science research laboratory - Instruments and their applications: / upezgsweyw
--Molecular biology lab: • Molecular biology lab

[@sugandhapant6937]

You're doing a great job sir 🙏

[@srinivasmohanty8859]

Amazing explained bro

[@mudavathpadma4976]

Thku so much sir 😊

[@greenhut1642]

IS IT POSSIBLE TO AUTOCLAVE IN NORMAL PRESSURE COOKER,

[@Dr.Rakesh.B]

Yes its possible.
Keep it in high flame for 3-4 whistles and leave it in simmer for 15 minutes and turn off.
We have done many times in cooker, tried and tested method.

[@greenhut1642]

Thankyou sir for the reply,

[@greenhut1642]

How can I contact you in WhatsApp, is it possible to assist me in tissue culture. Presently I had collected all materials for tissue culture. For doing it in my home.

[@greenhut1642]

i done with pressure cooker but the solution didnt solidify, 4gm agar i had used in 500ml,some precipitate were also seen after boiling. is bacteriological grade agar is used

[@Dr.Rakesh.B]

@greenhut1642 Its not because of pressure cooker. Add 5 to 6 gm of agar. Change agar and check once.
3 whistle and 15 minutes simmer.
It works great