Flow cytometry -3 | Acute lymphoblastic leukemia & lymphoma - What you NEED to know!!!
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- Опубликовано: 25 сен 2020
- This video is the third part of the lecture on flow cytometry and hematopoiesis. In today’s video of the flow cytometry series, we will be covering step by step journey of B-cell hematopoiesis and maturation with changes in CD markers and also the origin of all major B-cell neoplasms including B Lymphoma and acute lymphoblastic leukemia.
• Flow Cytometry - 4 | C...
the first video on flow cytometry:- • Flowcytometry Basics -...
the second video on flow cytometry:- • Flow Cytometry - 2 | H...
the third video on flow cytometry:- • Flow cytometry -3 | A...
the fourth video on flow cytometry:- • Flow Cytometry - 4 | C...
If you have missed our previous videos on the basics of flow cytometry where in part 1 we discussed working principles of the flow cytometry machine, interpretation of graphs, In part 2 of the flow cytometry series, we understood what CD markers are, and how hematopoiesis and cd markers lay down the foundation for understanding flow cytometry and interpretation of flow cytometry plots. We also learned cd markers of myeloid series on flow cytometry plots. If you have missed our previous two videos, there is a link you can see up, do watch them for having a better understanding of this flow cytometry lecture on hematopoiesis and B cell development and in no time, you can become a champion in the interpretation of flow cytometry
B-cell neoplasms are clonal tumors of mature and immature B cells at various stages of differentiation. Each b lymphoma and acute lymphoblastic leukemia subtype bears a phenotypic resemblance to B cell at a stage of differentiation during the hematopoiesis.
So instead of mugging different cd markers for b lymphoma and acute lymphoblastic leukemia, if we just remember the normal postulated counterpart from which b lymphoma and acute lymphoblastic leukemia arises, flow cytometry plots will NEVER BE A BRAIN TEASER AGAIN.
Now let’s move to the part we all want to learn- that is diagnosing b lymphoma on flow cytometry. Diagnosing b lymphoma and acute lymphoblastic leukemia by flow cytometry is a challenge. Let’s understand why?
Many a times in flow cytometry we get a sample from a lymph node or a peripheral blood to be tested for presence of lymphoma . However lymph node might be having reactive lymphoid cell proliferation due to infection , or peripheral blood lymphocytosis may again be reactive . So how do we differentiate both the conditions on flow cytometry plots? The answer is to learn the must know CD markers!!
Each lymphoma subtype bears a phenotypic resemblance to B cells at a particular stage of differentiation. So instead of mugging different markers for lymphomas , if we just remember the normal postulated counterpart from which lymphoma arises , identifying LYMPHOMAS on flow cytometry WOULD NEVER BE A BRAIN TEASER AGAIN.
So that’s all for today in flow cytometry. We sincerely hope that after listening to this video on flow cytometry in b lymphoma and b acute lymphoblastic leukemia, you all will be able to suspect and diagnose them on flow cytometry plots. If any doubts or queries regarding flow cytometry, please do write to us in comments section and we will love to hear from you. If you have not subscribed , make sure you do now so that you don’t miss your updates in oncopathology and hematology.
For basics and working of flow cytometry machine and interpretation of flow cytometry plots , you can watch our first video on flow cytometry series, a link of which is given in description below.
We hope you liked our video and stay tuned for next video on how to identify t cell lymphoms and t acute lymphoblastic leukemia on flow cytometry.
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Very well presented
Thank you
So happy to know you liked it divya !!! ☺️☺️☺️
I think it would be very helpful if you could do a tutorial showing actual flow charts illustrating specific conditions like various lymphomas, leukemias and other lymphoproliferative disorders. You could explain what exactly are the features that allows a hematologist to diagnose a specific condition by flow cytometry charts. I would like to add that all your previous videos have been excellent and congrtgulate you on doing an outstanding job. Do keep it up.
Thank you for making such a wonderful presentation… I am really very grateful to you
Excellent animations and clarity
Thankyou Dr Santosh ☺️
Very nice presentation. Very simplified. Would be lovely to see many more videos from you on flow cytometry.
Can't thank you enough. Very good video.
Excellent videos excellent animation & explanation. Very crisp and clear. Pls post more videos
Please make more presentations on other topics
Great work!
shotarozee glad to know you liked it ☺️
Excellent way to the most dreaded aspects of flow..interpretation of markers and their origins...very well explained and very lucid
Thankyou
Very nice presentation😊 Thankyou
Great work👍
Thank you so much shadab !!!
amazing
Very helpful
It ws ils so Nice thank you so much.
Awesomeee
thanks
I did a research that point that Cyμ should be negative in common B, that is correct ?
on the antigen independent phase of maturation you mentioned that the B cell pan markers are (CD19, CD22 and cytoplasmic) CD79a. all of the sudden on the antigen dependent phase you replaced CD22 with CD20 as a pan marker for B cell. Is this accurate? it is confusing, you didn't mention CD20 at all before this stage. Please clarify.
I have the same question
What if it’s positive for CD19, CD38 and CD45? And what does kappa and lambda mean?
Y is there’s background music?? That’s so distracting!!!
thank you so much!!!!
Thank you !! We stopped making because we didn’t get good response
@@pathogenesis4363 I'm sorry! Most people use valuable tools when they need them the most, but don't give thanks/credit when it is due
really great video...
will be highly obliged if make somthing on PNH,DHR and EMA dyebinding in flow cytometry
i do procedure in my department but principles are not very clear..
Bhartiy Deeksha thank you so much deeksha !!!
It’s students like you who keep us motivated to teach
Would do on pnh soon !!!
@@pathogenesis4363 thanks a lot
Am very late to this discussion but came across it as I was looking for an explanation in a case I have where CD4 count is .10 CD8 is .24 and CD3 is .66 (all 10*9/L). What I cant figure out is CD4 added to CD8 would only be .34 which makes CD3 194% of that. I thought Cd4 plus Cd8 should almost equal CD3. So, what then is expressing CD3 but is not a CD4 or CD4?
Great
Memory B cell is CD 23 negative OR positive???
Plz send me notes about that topic it's urgen6
Diagnosing B cell lymphoma 14:01
Do you have any query ?
Is it possible to get a PDF from your website.
Thanks.
Pdf of which thing sir ??
Please elaborate
@@pathogenesis4363 B cell development.
Either same video content in the form of power point.
Excellent work.
Keep the intro short otherwise nice work 👍🏻
Thankyou