sir there is a mistake in row E column H (Here comes 3 instead of 5 because when we substract 11-8(gap) we get 3), But you wrote 5, and there is also a mistake in the last cell where you wrote 27 but if we add E and E match score which is 6 we get 34 because we add diagnonal values when there is match. Plz respond and solve this query.
Dear sir, is totally right but where you have arise mistake, there is no mistake. If you see in the first mistake which you have mentioned,the value will come 5 because if you do 11- 8 = 3, but if you see in the blousum table E with H، the value is 0 , so the diagonal value is 5 . Then 5 - 0= 5 .. so 5 is greater that way he writes 5... In second mistake E is match with E the value is 6 .. so you will add the value with the diagonal, so in diagonal the value is 21 .. so 21 + 6 = 27.. so sir write 27
Hi sir. Is there any videos on how to align globally using blosum 62?
Sometimes you add a gap-penalty and sometimes you don't. Can you explain why?
Thanks it was really helpful
Pleasure
sir there is a mistake in row E column H (Here comes 3 instead of 5 because when we substract 11-8(gap) we get 3), But you wrote 5, and there is also a mistake in the last cell where you wrote 27 but if we add E and E match score which is 6 we get 34 because we add diagnonal values when there is match. Plz respond and solve this query.
Dear sir, is totally right but where you have arise mistake, there is no mistake. If you see in the first mistake which you have mentioned,the value will come 5 because if you do 11- 8 = 3, but if you see in the blousum table E with H، the value is 0 , so the diagonal value is 5 . Then 5 - 0= 5 .. so 5 is greater that way he writes 5... In second mistake E is match with E the value is 6 .. so you will add the value with the diagonal, so in diagonal the value is 21 .. so 21 + 6 = 27.. so sir write 27
mistake in W and E as 6-3 +3 BUT 12-8+4