Excellent! I finally understand it. However, one question: at 3:40 where the TALENs are biding to the DNA, it seems that the TALENs are too bulky to fit close enough together to bind to successive nucleotides.
It is more specific than CRISPR, but all nuclease technologies have a degree of off-target activity. This introductory talk briefly mentioned obligate heterodimer mutations. These greatly reduce off-target cleavage arising from binding to only one half-site. With these FokI mutations, TALENs are arguably the most specific platform. Specificity isn’t the only issue though, new technologies like prime editing, which avoids creating a DSB, also reduce unwanted repair outcomes. See my talk on that...
Watched your video on ZFNs and CRISPR as well, has helped a heap with my assignment. Thanks you for the great videos 👍
Great to hear Xavier. Best wishes for your studies 👍🏼
RVD stands for Repeat Variable Diresidue. The amino acids in the middle are called central TAL targeting domain.
Your explantions are very clear, I enjoyed your videos and learned a lot. Thank you very much!!!
Really enjoying this series of videos
Thank You
You're very welcome. Glad you're enjoying them!
Extremely helpful making my assignments much easier Thank you!!
Glad you found it useful Jack. Check out our other genome editing videos.
Great video; Shortly and very clearly explained. Thank you!
Thanks Stanley. Glad you found it useful!
Great video, detailed yet short thank you
Glad you found it useful!
Thank you for the very helpful video and good job on the channel!
Thanks for your kind feedback. Hope you enjoy the rest of the videos!
Very nice images!
it is so good ,very helpful
Very clear and helpful presentation. Thanks you so much :))
Thanks Karolina. Glad you found it useful.
Extremely helpful in making my topic discussion for one of my plant breeding subjects. Thank you so much sir!
Glad it helped. Best wishes for your studies!
@@GenomicsGurus Sir if I may. Does "predictable specificity" pertain to the exception of the 12th and 13th amino acids in the repeat?
Residues 12/13 in each repeat are the variable amino acids that make contacts with the bases and thus direct specificity
ITS REALLY A GOOD PRECISE AND HELPFUL VIDEO BE BLESSED
Glad it was helpful!
Thank you
Thank you you so much! :)
Glad you found it useful!
Excellent! I finally understand it. However, one question: at 3:40 where the TALENs are biding to the DNA, it seems that the TALENs are too bulky to fit close enough together to bind to successive nucleotides.
The successive domains curve round the the DNA helix like a propeller - it's an amazing structure
is this method susceptible to off target cleavage like with CRISPR enzymes?
It is more specific than CRISPR, but all nuclease technologies have a degree of off-target activity. This introductory talk briefly mentioned obligate heterodimer mutations. These greatly reduce off-target cleavage arising from binding to only one half-site. With these FokI mutations, TALENs are arguably the most specific platform. Specificity isn’t the only issue though, new technologies like prime editing, which avoids creating a DSB, also reduce unwanted repair outcomes. See my talk on that...
@@GenomicsGurus thank you for the help! :)
SIR PDF AWABILE THIS LECATURE
There is not.
Boka_____ amake aro guliye dilo