Alginate Encapsulation of PSCs Using a Co-axial Nozzle | Protocol Preview

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Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle - a 2 minute Preview of the Experimental Protocol
Ikki Horiguchi, Yasuyuki Sakai
The University of Tokyo, Center for International Research on Integrative Biomedical System, Institute of Industrial Science;
We established a method of encapsulating pluripotent stem cells (PS cells) into alginate hydrogel capsules using a co-axial nozzle. This prevents cells from aggregating excessively and limits the shear stress experienced by cells in suspension culture. The technique is applicable to the mass production of PS cells as well as research on stem cell niche.
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