your videos are insightful. I work at the hospital but I dont conduct lab tests; it is interesting to see/listen a report from a microbiologist on what they're working with.Please do continue making more. I also took microbiology at my university, very challenging but rewarding class.
Great video. I have some remarks if that is ok. I suggest the use of one drop of drop-oxidase on the BAP plate just to rule out any Aeromonas, Plesiomonas, vibrio, or any other oxidase-positive enteric pathogens. I use this method for quicker preliminary identification. I would use an EMB plate instead of MacConkey because the green metallic sheen and the purple color would help me quickly eliminate usual flora and just focus on the pink colonies for further identification. I would include a CIN plate for the recovery of Yersinia sp., Aeromonas, and Klebsiella oxytoca which is present in cases of antibiotic-associated hemorrhagic colitis. Finally, I would include a CVA plate for the recovery of Campylobacter sp. Again, thank you so much for the video.
Hi Veronika and thank you. It's been a while since I made these videos, but I think the Stool II video does address pathogens meaning lactose-negative and/or H2S-positive colonies.
It doesn't look like there are any clear colonies on the HE plate, so these organisms are ultilizing lactose and/or sucrose. Salmonella and Shigella do not utilize lactose or sucrose, so more than likely these colonies are not Salmonella or Shigella.
Hi Hana, on stool cultures we fist look for lactose-negative colonies (possible Shigella) and H2S-positive colonies (possible Salmonella). We also look for a predominance of other organisms. For example if there was a majority of a yeast, that would be abnormal and should be investigated. Other stool media like MacConkey sorbitol and Campylobacter agar would have other work-up parameters.
@@patricktracy9947 There are lactose-negative colonies too there (on the edge of the plate). I am still confused and can you make a video about stool cultures that are concerning as in pathogenic? Thank you!
@@hanazhafirahhanifah8175 Sometimes what happens is the lactose+ colonies in quadrant 1 of the plate start to change color back to being clear. They use all the lactose in the first 24 hours of incubation and turn yellow, and then start using other nutrients and turn clear or non-yellow.
Hi Mark...every lab will have its own standard operating procedures which will include how to report results. My videos are only an example of what is commonly examined. Thank you for your input.
your videos are insightful. I work at the hospital but I dont conduct lab tests; it is interesting to see/listen a report from a microbiologist on what they're working with.Please do continue making more. I also took microbiology at my university, very challenging but rewarding class.
Thank you so much.
your videos are very illustrative and amazing plz do a video on
ELISA
good video
Thank you. Keep continue please
Hi
By the way good videos all of your plate reading videos.
Keep it up sir😊
I am glad they were helpful.
Well-done sir.
Thank you. I hope it was helpful.
Great video. I have some remarks if that is ok. I suggest the use of one drop of drop-oxidase on the BAP plate just to rule out any Aeromonas, Plesiomonas, vibrio, or any other oxidase-positive enteric pathogens. I use this method for quicker preliminary identification. I would use an EMB plate instead of MacConkey because the green metallic sheen and the purple color would help me quickly eliminate usual flora and just focus on the pink colonies for further identification. I would include a CIN plate for the recovery of Yersinia sp., Aeromonas, and Klebsiella oxytoca which is present in cases of antibiotic-associated hemorrhagic colitis. Finally, I would include a CVA plate for the recovery of Campylobacter sp.
Again, thank you so much for the video.
What's CIN?
@@Blevic18 Cefsulodin-Irgasan-Novobiocin (CIN). It's a selective and differential medium for the isolation of Yersinia enterocolitica.
@@Blevic18 Cefsulodin Irgasan novobiocin (CIN) agar
@@Blevic18 Cefsulodin Irgasan novobiocin (CIN) agar
I think he used SMAC media so he did not need to use EMB
I liked this video, but I think it would more interesting to see pathogens on those plates for comparison. Thank you
Hi Veronika and thank you. It's been a while since I made these videos, but I think the Stool II video does address pathogens meaning lactose-negative and/or H2S-positive colonies.
Pls clarify HE plate non lactose colonies are there or not.. If present so why mac plate no non lactose colonies..
It doesn't look like there are any clear colonies on the HE plate, so these organisms are ultilizing lactose and/or sucrose. Salmonella and Shigella do not utilize lactose or sucrose, so more than likely these colonies are not Salmonella or Shigella.
I am still confused how to differentiate normal flora with pathogen because to me, all of these plates contain a lot of colonies.
Hi Hana, on stool cultures we fist look for lactose-negative colonies (possible Shigella) and H2S-positive colonies (possible Salmonella). We also look for a predominance of other organisms. For example if there was a majority of a yeast, that would be abnormal and should be investigated. Other stool media like MacConkey sorbitol and Campylobacter agar would have other work-up parameters.
@@patricktracy9947 There are lactose-negative colonies too there (on the edge of the plate). I am still confused and can you make a video about stool cultures that are concerning as in pathogenic? Thank you!
@@hanazhafirahhanifah8175 Sometimes what happens is the lactose+ colonies in quadrant 1 of the plate start to change color back to being clear. They use all the lactose in the first 24 hours of incubation and turn yellow, and then start using other nutrients and turn clear or non-yellow.
Why not use SSA or Xld plate for stool culture 🤔
Hi Rami, yes I could use those, but Hektoen-enteric agar does the same thing. It is used for stool pathogens just as SSA and XLD are.
I think you should report that as a "NO ENTERIC PATHOGEN ISOLATED"
Hi Mark...every lab will have its own standard operating procedures which will include how to report results. My videos are only an example of what is commonly examined. Thank you for your input.