JID June 2024 Cover: Mammary bud epithelium (TO EDIT)

mammary bud, hair follicle, tissue recombination, Sox2
Mammary bud epithelium adopts hair follicle cell fate upon contact with the hair follicle dermal condensate. E13.5 mouse mammary bud epithelium expressing mGFP (cyan) was manually separated from the surrounding mammary mesenchyme after enzyme treatment, and recombined with the E16.5 back skin mesenchyme dissected from a mouse embryo ubiquitously expressing mTdtomato (magenta). The recombinant was cultured for 6 days before fixation and whole-mount immunostaining with the dermal papilla marker Sox2 (yellow). Z-stack images were captured using Leica SP8 Upright confocal microscope with an HC PL APO 20x/0.75 IMM CORR CS2 objective. For more detail, see the article “Stabilization of epithelial beta-catenin compromises mammary call fate acquisition and branching morphogenesis” by Satta et al doi.org/10.101....