Why DAPI only binds to the minor groove AT rich region, And not GC regions? What brings such a great affinity that it's a specific property of DAPI dye. ?
Dapi stains all DNA and for instance you can see blue little dots on cell culture dishes when they are contamined with mycoplama bacteria. Mitochondrions are also stained but the DNA is small and is not as easy to visualise than the large eucaryotic nuclei.
@@novabiotis3993 I guess that explains why DAPI staining is so bad in yeast cells. The whole cell is blue and I can just barely see a darker blue where the nucleus is.
@@thefenerbahcesk4156 Yeast are the smallest eucaryotic cells ! Only 5 micromètres.. so, il your staining is too strong, all thé cytosol will be fluorescent .
Why DAPI only binds to the minor groove AT rich region, And not GC regions? What brings such a great affinity that it's a specific property of DAPI dye. ?
Very useful explanation. Thank you
how does a dead cell that underwent apoptosis appear under the microscope? How to differentiate them from the healthy cells?
Woow cool learn info from student mit edu.
Thank you
Why doesn't DAPI stain mitochondria?
Dapi stains all DNA and for instance you can see blue little dots on cell culture dishes when they are contamined with mycoplama bacteria. Mitochondrions are also stained but the DNA is small and is not as easy to visualise than the large eucaryotic nuclei.
@@novabiotis3993 I guess that explains why DAPI staining is so bad in yeast cells. The whole cell is blue and I can just barely see a darker blue where the nucleus is.
@@thefenerbahcesk4156
Yeast are the smallest eucaryotic cells ! Only 5 micromètres.. so, il your staining is too strong, all thé cytosol will be fluorescent .