TNP #34 - Zeiss Microscope Motorized XYZ Axis Stage Focus Stacking & Image Stitching Tutorial
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- Опубликовано: 5 май 2023
- In this episode Shahriar continues his journey with upgrading the Zeiss microscope. The microscope is now entirely motorized including the XYZ axis. This, combined with a high-quality Nikon DLSR allows the creation of some extraordinary focus stacked imaged. The interface between the microscope & DSLR is handled through an LMScope adapter:
www.lmscope.com/en/produkte_e...
The microscope is controlled via a custom GUI which manages all of the microscope functions as well as a fully automated focus stacking routine.
www.TheSignalPath.com
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It might be worth disabling auto white balance on the camera. It looked like the layers were changing colour slightly as the focus changed which is probably caused by this. I'd probably also set a fixed ISO and if shoot in RAW if it's supported by your focus stacking software. I can't imagine JPEG artifacts are ideal for focus stacking - especially with all the sharp lines you see in ICs.
Great advice here!
Wow, that kit must have cost a pretty penny.
Absolutely great video, I love your videos on optics. Recently I bought an old but fancy Leica fluorescence microscope. It's missing the xyz controller, so this video certainly contains some useful info for me. Thanks.
I do lots of focus stacking of plants and small insects. Since you have stack and stich capabilities, I would recommend looking at zerene stacker as a software to use. It has much better batch processing and retouching tools. It is much easier to set up a batch job in zerene, and have it automatically stack all the images and save them out for stitching for me. I would also recommend trying out the focus stacking capability in Affinity Photo. Helicon and zerene both give you the option to use a depth map or pyramid maximum algorithm, but affinity photo seems to use a hybridized method which (I think) combines both stacking algorithms, making some images have less halo artifacts without having to retouch. Affinity photo also has stitching which has worked pretty well for stacked images for me.
Another great video. Cheers.🍻
I was outbid on a 3 port microscope with a motorised xy stage but missing the controller that went for over EUR500.
Your results are amazing and perhaps I should have bid a bit more.
Talking to us like it hasn't been two _years_ since the last video about this microscope... lol. You over-estimate my memory.
Man, I wish we had nice scope like this...I can stare at these mmic for days.
beautiful
Nice video. Back at a previous job we had a zeiss axioplan(I think) that was super nice with darkfield and a motorized stage and even a motorized objective changer. It was one of our nicest visual optical microscopes, as opposed to our camera only microscopes(as in one with no eyepieces). I remember we also had a Keyence VR-X000(or similar) with 3d profilometry, 2d stitching and, multi-focal point stacking. Man I wish I had one of those still. Who knows we(my former employer) might have made some of those passive devices in the Keysight synthesizer, though Keysight probably made them internally.
That is a pretty impressive control program that you wrote(I think that was what you said).
Very cool stuff. Looks like a great improvement over your previous setup.
Спасибо за очень интересные ролики с хорошим оборудованием.
Great video, as always!!! Reminds me of the time when i did this as a student 30 years ago 😅 this was way harder with the technology we had back then. Thank you so much and keep up the good work!
amazing images, really great. Didnt know that the focus stacking works so well.
Very nice it is, somewhat hard focus stacking by hand!
I am well impressed.
I did some focus stacking in Photoshop with a 105mm macro lens in the past, it's amazing. I'll definitely check onto that software though. Thanks.
One function that would be interesting to do would be a slow feature of CHDK for old canon point and shoot cameras. Basically they had an option to do focus stacking where each stage could have exposure bracketing for making a focus stacked HDR image. It didn't catch on as much back then since it used the mechanical shutter, but with electronic shutters there is no worry about wearing out a shutter when a massive number of images are captured.
Awesome !....cheers.
Nice
Wow that pc software looks sweet, what software framework did you use to create those knobs?
Looks like ~~labview~~. Correction. That is written in matlab.
Yes, Matlab.
That stage looks pretty awesome. I have an AmScope Trinocular scope and it was an absolute pain to find the right combination of parts to get the camera in alignment/focus... would be nice to attach one of my M4/3 cameras to it to get better image quality. Would be interesting to hear you talk about your ASIC projects sometime (eg. the computer design, how/where they were manufactured, etc.).
16:03 this is kinda SMD with dies, very cool en pretty. I wish I was brave enough to work with fuming nitric accid and had the money for a Xray machine. I repair electronics (Measurement/calibration/maritime gear) for a living and often I am curious what went wrong inside a component. On the other hand, I would probably spending more time doing that then repairing :-)
👏👏👍
Fantastic setup! Can we assume you've got some fancy MEMs devices to show off the capabilities of it next?
I've also been getting into focus stacking microscope pictures recently as well but nothing anywhere near as fancy, just with a microscope objective directly mounted to a camera on a tube extension. Pictures are no where near as high quality but still good enough and attainable for the average hobbyist.
It's just amazing what humans can build. I'm such a poor guy compared to those real geniuses around the world.
What kind of scale is used for positional feedback? Is there an online service manual?
I wonder whether it's possible to stick regular image stacks with darkfield image stacks...
All these little squares on your chip, what are they intended for? Are they maybe there to help uniform etching?
They are metal fills. Required to meet a uniform density specs for the fabrication process.
I guess you spent part of your postdoc in Japan? Wasn't expecting to see Japanese.
Can you slightly tilt the specimen and make a photo from two angles to create a stereo image?
Are you open sourcing the GUI?
More you show this microscope more I won't it 😆
The japanese text says "Hugo"
Is not better a Global Shutter camera like, for example, the Flir Chameleon3 cameras, instead of a DLSR? When you get used to the global shutter, you can't stand seeing moving pictures showing rolling shutter again.
I can get 60FPS from this camera and with such a large sensor I get very little noise at low-light. So rolling effects are minimized.
How do you afford all this stuff!
ヒューゴ Hugo?
Yes, my middle name.
2:41 It probably knows this because it knows where it isn't...
is it possible to add HDR too?
It’s definitely possible! I started doing something like that for a project at work.
Surprised how janky the software is. It's still a fuzzy process of edge detection to correlate pixels with layers.
.... So, I can do all this with a $100 AmScope microscope, right? 😂
You actually could. The results wouldn’t be as good, but nothing stopping you
That's a pretty fancy milling machine you have there. Although, I don't see how you're going to attach The cutting head.
just shoot lasers through the microscope lens.