Some chicken left in a safety cabinet for a few weeks makes a good Grocott/Extended PAS fungi control, although a little on the ‘strongly’ positive side.
I guess because it’s a small hollow tube that is easy to work with and we have lots of excess cord all the time in the lab. I’ve heard appendix can be used in similar way also.
This is awesome. Can your lab cultivate Helicobacter pylori or Treponema and use the same method for creating controls for H pylori IHC and Warthin Starry/ Treponema IHC ?
I have heard that it can be done with Helicobacter (if I recall correctly, someone told me they used to do that and put it into appendix as a control). But I don’t think that Treponema palladium can be routinely grown and culture in the laboratory. It is always a struggle to find good syphilis controls. In my lab, I am lucky enough to have the spirochete immunostain for Treponema so thankfully, I never have to use the warthin starry stain which I find very difficult to interpret!
Dr Jerard, I would like to know if you decontaminate your tissue processor after running the blocks. I work in Clinical setting and worry about the risk of contaminating other blocks. (I sent you an email as well) thanks 🙏🏻
The organisms are fixed in formalin so they are dead. So I don’t think any need to decontaminate (also processor runs formalin and absolute alcohol through it so it basically decontaminates itself with each run). It’s different than cutting fresh tissue with TB or other AFB in a cryostat where decontamination is needed. I suppose small pieces of agar with organisms could break loose and get into other blocks (which is a risk with any specimen). I haven’t seen that happen and even if it did, it would be pretty obvious to the pathologist microscopically that it was a piece of floating agar with AFB from control block rather than real infection.
Some chicken left in a safety cabinet for a few weeks makes a good Grocott/Extended PAS fungi control, although a little on the ‘strongly’ positive side.
Thank you so much
Excellent content! Thanks for sharing! May I ask why umbilical cord was chosen instead of some other tissue?
I guess because it’s a small hollow tube that is easy to work with and we have lots of excess cord all the time in the lab. I’ve heard appendix can be used in similar way also.
This is awesome. Can your lab cultivate Helicobacter pylori or Treponema and use the same method for creating controls for H pylori IHC and Warthin Starry/ Treponema IHC ?
I have heard that it can be done with Helicobacter (if I recall correctly, someone told me they used to do that and put it into appendix as a control). But I don’t think that Treponema palladium can be routinely grown and culture in the laboratory. It is always a struggle to find good syphilis controls. In my lab, I am lucky enough to have the spirochete immunostain for Treponema so thankfully, I never have to use the warthin starry stain which I find very difficult to interpret!
Muito bom.
Dr Jerard, I would like to know if you decontaminate your tissue processor after running the blocks. I work in Clinical setting and worry about the risk of contaminating other blocks. (I sent you an email as well) thanks 🙏🏻
The organisms are fixed in formalin so they are dead. So I don’t think any need to decontaminate (also processor runs formalin and absolute alcohol through it so it basically decontaminates itself with each run). It’s different than cutting fresh tissue with TB or other AFB in a cryostat where decontamination is needed. I suppose small pieces of agar with organisms could break loose and get into other blocks (which is a risk with any specimen). I haven’t seen that happen and even if it did, it would be pretty obvious to the pathologist microscopically that it was a piece of floating agar with AFB from control block rather than real infection.
@@JMGardnerMD thanks so much! 🙏🏻I appreciate you taking ge time to reply.