pUC Vector: Features, Selectable Markers || How Blue White Recombinant Screening works? Explained
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- Опубликовано: 9 июл 2024
- 5 Minute Video Please subscribe using the link: bit.ly/3kG2kKf
0:20 What is pUC vector and Full formed and developed by?
0:55 Features of pUC vector
1:43 Where gene of insert is inserted in PuC vector? Insertional inactivation
2:43 Blue White colony screening
Advantages of pUC vector
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Hi Sohini Samanta
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Why white and why blue ........
What is the chemical reaction....
Hi Prasenjit Sahoo
β-Galactosidase cleaves X-gal into galactose and 5-bromo-4-chloro-3-hydroxyindole; this second compound is then oxidized into 5,5′-dibromo-4,4′-dichloro-indigo. As its name suggests, this final product is blue in color.
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