Selectable Marker: Definition || How Selectable Marker helps in selection of recombinant colonies?||
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- Опубликовано: 24 июл 2024
- A 5 minute video What is the purpose of Selectable marker?||
Steps in rDNA technology: • Steps in Recombinant D...
0:00 Introduction
0:30 Selectable Marker: Definition What is a selectable marker
0:50 Selectable Marker explanation with pBR 322as example
1:33 Selectable Marker and restriction site; insertional inactivation
2:45 How insernational inactivation helps in the selection of recombinant colonies
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Thank you so much
Thank you so much sir. Your explanation on selectable markers was very helpful!
Very nice explanation sir this was the most difficult topic for me but now it's very clear thank u
Thanks a lot sir , I clearly understood this, it's fully doubled before now it is crystal clear ❤️🔥🔥🔥
Love from kerala
Good explanation sir
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Thank you sir😊
Thanks sir for explainng what other utube vdos didnt explain😀
This is very helpful. Thank you so much.
Thank you very much for the explanation. 😊😊
Thank you very much for this vedio. Very helpful
Thank you so much this video is very helpful 😊
Nice explanation 👌👍Thanks... I was in search of this kind of video only... 😊
ME TOO
Thanks sir after long time confusion dur
Very helpful dir
Thank you sir
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Glad to hear that
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Amazing explanation 🙏🙏🙏🙏🙏
Hi Tanvi
Thanks and welcome
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Too good👍
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What's use of this type of selection.....
Reply me please
Thank you so much sir
Most welcome
Thank u so much sir
Most welcome
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Hello, I want to ask that if the red colonies and the green one (according to your color codes) look the same on the Ampicillin plate, how can we select for the one that has the insert?
Its by the position of the colonies. We use replica plating method. this video may help you ruclips.net/video/xcbrtkFn7-U/видео.html
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thank u sir
Hi demi g0d3ss
Welcome...
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Sir! Is master plate have tetracycline and ampicilin?
Master plate is the original culture without any antibiotics. We need to keep it intact to find out the recombinant colonies. That's why we use replica plating to find out colonies growing in antibiotic containing medium.
how to determine which is recombinant/non-recombinant within a colony??
That step is comparatively easy. if we grow the colonies in an antibiotic containing medium, Only the recombinants can grow as it contains vector that contain antibiotic resistance genes like tetracyclin or ampicillin (selectable marker).
The next step the selection of transformed colonies with unchanged vector and recombinant vector with our gene of interest. Suppose our gene of interest is inserted in Tetracycline region, that gene is no more active (called as insertional inactivation). Therefore the one with recombinant vector can grow only in Ampicillin containing medium but cannot grow in Tet containing medium. This is how selection works. I think the image in the video will help you...
Just sharing a video on pUC vector for your reference. ruclips.net/video/SxKQ65b2J18/видео.html
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@@biologyexams4u if we treat the recombinant vector with tet then the bacteria will die then how will we obtain our gene of interest
I have the same doubt
Why non transformed gene will not grow in both medium?
Non transformed means bacteria without our vector. So It doesn't have antibiotic resistance gene. So normally it cannot grow in antibiotic containing medium.... Hope I clarified.. Thank you
Yes now I understood
@@biologyexams4u if we will use EcoRI then which selectable marker will be there, coz in EcoRI no selectable market is present in its region?
OKAY...but where is insertional inactivation here -_-
Your sound is very bad😓😓
Really appreciate your sincere words....Hope the content helped your understanding...
Thank you so much for your sincere comment:)
Simple explanation.Thankyou so much
Sir here in this case selectable marker is ampicillin resistanct gene or tetracycline resistant gene?
Both here one helps in insertion of gene and other helps in selection. We can use any of these as selectable marker
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