Novel Branching Method for Solid Phase Peptide Synthesis

TIDES USA 2024 John Lee, Associate Director Innovation. At present, Polystyrene resin based solid phase peptide synthesis (SPPS) is the “go to” method for both research and industrial scale peptide syntheses. At industrial scale however, the current SPPS introduces major drawbacks; large reactor requirements and enormous amount of chemical wastes generated making synthetic peptides one of the most expensive molecules to produce. We report highly effective “multiple dendrimeric” constructs for the use in SPPS. With the novel branching arrangements with “spacers”, we demonstrated product yield up to 8-fold increased compared to traditional method of SPPS. Peptide size up to 50 amino acid long in length with comparible purity to conventional peptide synthesis has been demonstrated. Furthermore, we show that this approach reduces reactor size which gives signficant application advantages to industry for reducing wastes and facility size.