How Does Chick ex ovo Culture and Ex ovo CAM Assay Work?

Chicken eggs in the early phase of breeding are between in vitro and in vivo systems and provide a vascular test environment not only to study angiogenesis but also to study tumorigenesis. The video describes the establishment of a refined chick ex ovo culture and CAM assay with survival rates over 50%. Besides it provides a step by step demonstration of the successful application of the ex ovo culture for a large number of scientific applications.

[@jakob1840]

Nice video very informative

[@dimisiks1936]

Hello, so this method doesn't require CO² incubator?

[@Educationalcourses]

It is not necessary if the incubator is not closed hermetically (ventilation grid partially open!) and special Petri dishes with spacers between lid and dish are used.