hello doctor please can you helpe me I am a phd student and i work with embryonated eggs for cultiving the parasits inside the chorioallantoïc fluid I find a obstacle how can I do for injecting the parasitis specifically inside this fluid
Hello! I am a 12th grade student doing a thesis about whether the extract of a dragon fruit stem has anti-angiogenic properties via CAM assay. This video has been pretty useful so far. But I have a few questions to ask: 1.) In this video, you used chicken eggs... Is it still the same process if we use duck eggs? 2.) When do people usually apply a certain extract (in this case our dragon fruit stem extract) into the egg? What’s the process of that? Are there any considerations or requirements? 3.) What specific kind of tape did you use? Moreso, what type of scissors did you use to cut the eggshell? (very impressive by the way) Sorry for the questions, I’m a graduating student and I really need this to work… I probably have other questions still but I’m still working on getting my proposal approved.
1. We see some sort of vessels autolysis in some embryos(quite often). 2. Egg content becomes cloudy and turns into a paste(also often). We are suggesting contamination or lack of O2. Maybe you have faced such things and have some tips?
@@dmytrokrasnienkov1645 I suggest sealing the window so tight to prevent any air bubbles, also during the windowing process, many eggs developed cracks in the shell so carefully check up the egg after windowing.
Regarding the 10-day chicken embryo : I noticed ( in the same batch of eggs) that some of the embryos look normal but others are surrounded by blood and even the embryo skin is red -looks like is full of blood. Why is that ? thanks
I think that means the embryo died. Slowly guide the lower blade of the scissors into the egg being sure to keep the tips up against the inside of the shell so, you don't hurt the embryo.
@@Educationalcourses After I took out the embryo I thought it was about a kind of disease of the hen or because the food of the hen - the embryo seems to be full of blood. and it has no life- not any movement- but it has at least 50% of the size of a healthy embryo .
hello doctor please can you helpe me I am a phd student and i work with embryonated eggs for cultiving the parasits inside the chorioallantoïc fluid I find a obstacle how can I do for injecting the parasitis specifically inside this fluid
Why not tape a piece of seran wrap over the whole do you don't have to tape a retape the door to look?
hello doctor please can you helpe me I am a phd student and i work with embryonated eggs for cultiving the parasits inside the chorioallantoïc fluid I find a obstacle how can I do for injecting the parasitis specifically inside this fluid
Hello! I am a 12th grade student doing a thesis about whether the extract of a dragon fruit stem has anti-angiogenic properties via CAM assay. This video has been pretty useful so far. But I have a few questions to ask:
1.) In this video, you used chicken eggs... Is it still the same process if we use duck eggs?
2.) When do people usually apply a certain extract (in this case our dragon fruit stem extract) into the egg? What’s the process of that? Are there any considerations or requirements?
3.) What specific kind of tape did you use? Moreso, what type of scissors did you use to cut the eggshell? (very impressive by the way)
Sorry for the questions, I’m a graduating student and I really need this to work… I probably have other questions still but I’m still working on getting my proposal approved.
For question1: yes, I think you can use the same procedure
For question 2: It differs depending on your study and what you want to observe. So, I advise you to talk to your supervisor about that matter.
for question 3: It is 3M clear plastic tape, the scissors are straight 4" dissection scissors.
@@Educationalcourses Thank you so so much!
Thank you for this video. We have tried it, but we have few problems:
1. We see some sort of vessels autolysis in some embryos(quite often).
2. Egg content becomes cloudy and turns into a paste(also often).
We are suggesting contamination or lack of O2. Maybe you have faced such things and have some tips?
@@dmytrokrasnienkov1645 I suggest sealing the window so tight to prevent any air bubbles, also during the windowing process, many eggs developed cracks in the shell so carefully check up the egg after windowing.
Regarding the 10-day chicken embryo : I noticed ( in the same batch of eggs) that some of the embryos look normal but others are surrounded by blood and even the embryo skin is red -looks like is full of blood. Why is that ? thanks
I think that means the embryo died. Slowly guide the lower blade of the scissors into the egg being sure to keep the tips up against the inside of the shell so, you don't hurt the embryo.
@@Educationalcourses After I took out the embryo I thought it was about a kind of disease of the hen or because the food of the hen - the embryo seems to be full of blood. and it has no life- not any movement- but it has at least 50% of the size of a healthy embryo .
What are the survival rates of the eggs ?
This method allows survival to E10 or later
Thanks
hello doctor please can you helpe me I am a phd student and i work with embryonated eggs for cultiving the parasits inside the chorioallantoïc fluid I find a obstacle how can I do for injecting the parasitis specifically inside this fluid