Seems easy enough! How important is the ph of the water? Is a lower temperature than 25 degrees just going to slow the growth or is it essential to have that temp? How come the culture in the vials is not green and why only dimmed light at first? Sorry so many questions I need to know everything! Thanks!!
Yeah algae is quite an easy thing, especially Freshwater. At a lower temp it would just be slower growth although too cold and it would inhibit the growth. It is a very pale green, may be hard to detect on camera, but as it grows it will darken in green, since setting up my culture the water is already starting to look more green tinged. I'm not entirely sure why the dimmed light at first honestly, the pH should be around 7, which with treated tap water that should be fine, I wouldn't worry too much about trying to add chemicals to alter the pH, as it is just adding more into the water that is passed on to the fish/whatever you are feeding the algae. No need to apologise for asking questions it is always encouraged. 😊
In terms of the growth medium I'm not sure you can make your own, but should be okay without it, if you are growing it to eat it may not be suitable to use anyway. You can get an algae harvesting screen to separate the algae from the culture medium, I hope this helps
I like it. But everything you have used here is that really foodsafe? I saw a skeleton head on one of the flasks. And i want to buy a culture to start my own. But i life in the Netherlands and i cannot find anny chlorella vulgaris or the chlorella pyrenoidosa algae. Only the suplements. I need life chlorella. Can you help me?? I really hoping for you to answer because i have looked and searched every were. And most youtube videos about chlorella do not show where to buy the life cultures. You had them in those two little flasks and you failed to tell us where you got them. Greetings,, Kitty.
Great video, how long will the culture survive for in the bottle or rather how long until you will need to upgrade the bottle. Are you growing it for personal interest or food for ?
Thank you! It will survive quite a long time in there, it depends on how quickly it will grow, once it hits a very dark green colour, I will either harvest some or change it to a larger container, the colour indicates the density basically. I am growing it for personal interest and for looking at under the microscope.
Hi Miss, I'm very very interested in the subject I have to do an open culture of chlorella v. exactly; it's for a project but I am very poor in English suddenly I understand nothing. if you could do a transcription it would help me a lot ps: I used google trad
In today's video I am setting up a Chlorella Vulgaris culture, C. VULGARIS is a species of Freshwater algae. So what is algae, what is phytoplankton? Phytoplankton is plankton consisting of microscopic plants such as algae, there are various species/genus of algae,this is what makes up Phytoplankton. Today I am focusing on chlorella vulgaris, a species of Freshwater algae,it grows in Freshwater (no salt) like tap water. Phytoplankton are marine microscopic algae,there are many different species both Freshwater and marine. Algae is at the bottom of the food chain, so supports life everywhere, all the way up to great sharks. Zooplankton, microscopic animals, feed on the phytoplankton, which are then eaten by small fish and crustaceans which are then eaten by larger fish/small sharks which are then eaten by apex predators, all the way up the food chain algae is what supports life. Algae is vital for life, and very important. Different algae looks different under the microscope, spirulina looks like little spirals/snakes, Chlorella looks like tiny, round very green circles under the microscope. Different algae can be fed to different species, Freshwater algae like Chlorella can support Freshwater species. Chlorella is a single celled green algae, it is photosynthetic although requires quite dim light for the first few weeks,and after that brighter light but not direct sunlight. The ideal temperature is 25°c (77°f), so a heating mat is highly recommended, but you can also find a warm spot without direct sunlight, and dim light for the first few weeks. One important thing to remember about chlorella, is that it will sink to the bottom of your culture jar, so make sure you give it a shake/invert it a few times to make sure it stays within the water column. What you will need: Chlorella starter culture Tap water Tap water conditioner Cultire jar Measuring jug Small measure (for tap water conditioner/growth nutrient) Optional but recommended: Growth nutrient Starting the culture: I washed my hands and put gloves on so not to contaminate the culture,I have an algae culturing manual from algae research and supply in the USA, it explains about different algae and their key features. I am using tap water, I don't want to use standard tap water due to risk of contaminants so am going to use tap water conditioner, I have a jug of 500ml of tap water, I use aquacare or API tap water conditioner. Due to them being so concentrated I am making up a much larger amount than I will actually need as it makes measuring simpler. I tend to make up 1 litre and then save it. I am using aquacare today it is 1 capful (5ml per 20litres) so I only need a tiny amount. I am using 0.01 for 500ml. I ensured there were no air bubbles and then squeezed this into the water, using the syringe to mix it in. So here are the culture vials, I have two of these. I have a culture flask, I am starting off small and will be upgrading to larger containers as I go along. I unscrew the lid and tap the algae vials into the culture container, although not done in the video I advice using a pipette for this. I am now topping up the culture flask with the treated tap water, now there is just one last thing to add which is the growth nutrient, that is kept in my fridge. This is my phytoplankton nutrient from ReefPhyto where I get most of my algae culturing supplies from, 3ml per 2 litres of water, I will be adding 1ml, 6ml can be added for your first 2 litres as the amount can be doubled, so 1ml should be fine, a little extra shouldn't matter too much. This nutrient is based on the Gulliard f2 medium,with a fresh syringe i am drawing up 1ml and putting this straight into the culture flask. This will now go back in my fridge. I am just shaking the flask a little to help with the aeration. So it is photosynthetic but requires dim light for the first few weeks,so I am going to put it in my bedroom where there is some light but it is mostly dim. So that is how to set up a basis chlorella culture. It does need air so I aerate by shaking the culture flask.
Seems easy enough! How important is the ph of the water? Is a lower temperature than 25 degrees just going to slow the growth or is it essential to have that temp? How come the culture in the vials is not green and why only dimmed light at first? Sorry so many questions I need to know everything! Thanks!!
Yeah algae is quite an easy thing, especially Freshwater. At a lower temp it would just be slower growth although too cold and it would inhibit the growth. It is a very pale green, may be hard to detect on camera, but as it grows it will darken in green, since setting up my culture the water is already starting to look more green tinged. I'm not entirely sure why the dimmed light at first honestly, the pH should be around 7, which with treated tap water that should be fine, I wouldn't worry too much about trying to add chemicals to alter the pH, as it is just adding more into the water that is passed on to the fish/whatever you are feeding the algae. No need to apologise for asking questions it is always encouraged. 😊
@@TeaandtheDeepbluesea alright thanks! I feel sufficiently prepared now! I hope my inverts who will eat it will be happy!
@@criminalanimal sounds like it. I'd love to hear how you get on
Hello!! Is there any way we can make growth nutrient at home?
How can i make my own food to feed the culture and how to harvest when I want to eat
In terms of the growth medium I'm not sure you can make your own, but should be okay without it, if you are growing it to eat it may not be suitable to use anyway. You can get an algae harvesting screen to separate the algae from the culture medium, I hope this helps
I like it. But everything you have used here is that really foodsafe? I saw a skeleton head on one of the flasks. And i want to buy a culture to start my own. But i life in the Netherlands and i cannot find anny chlorella vulgaris or the chlorella pyrenoidosa algae. Only the suplements. I need life chlorella.
Can you help me??
I really hoping for you to answer because i have looked and searched every were.
And most youtube videos about chlorella do not show where to buy the life cultures.
You had them in those two little flasks and you failed to tell us where you got them.
Greetings,, Kitty.
Great video, how long will the culture survive for in the bottle or rather how long until you will need to upgrade the bottle.
Are you growing it for personal interest or food for ?
Thank you! It will survive quite a long time in there, it depends on how quickly it will grow, once it hits a very dark green colour, I will either harvest some or change it to a larger container, the colour indicates the density basically. I am growing it for personal interest and for looking at under the microscope.
@@TeaandtheDeepbluesea you doing a video on the culture under the microscope 🔬?
Most definitely, got a lot of microscope stuff to film, I have quite a few algae cultures
How much time it takes?
Wonderful! I’ll be doing this for my smoothies 😌 Minus the tap water stuff, I’ll use a CO2 mineral water instead
Hi Miss, I'm very very interested in the subject
I have to do an open culture of chlorella v. exactly; it's for a project
but I am very poor in English suddenly I understand nothing.
if you could do a transcription it would help me a lot
ps: I used google trad
Hello, Thank you for your comment. I can work on a transcript for you no problem, I will get that done and comment back when it is done:)
In today's video I am setting up a Chlorella Vulgaris culture, C. VULGARIS is a species of Freshwater algae. So what is algae, what is phytoplankton? Phytoplankton is plankton consisting of microscopic plants such as algae, there are various species/genus of algae,this is what makes up Phytoplankton. Today I am focusing on chlorella vulgaris, a species of Freshwater algae,it grows in Freshwater (no salt) like tap water. Phytoplankton are marine microscopic algae,there are many different species both Freshwater and marine. Algae is at the bottom of the food chain, so supports life everywhere, all the way up to great sharks. Zooplankton, microscopic animals, feed on the phytoplankton, which are then eaten by small fish and crustaceans which are then eaten by larger fish/small sharks which are then eaten by apex predators, all the way up the food chain algae is what supports life. Algae is vital for life, and very important. Different algae looks different under the microscope, spirulina looks like little spirals/snakes, Chlorella looks like tiny, round very green circles under the microscope. Different algae can be fed to different species, Freshwater algae like Chlorella can support Freshwater species.
Chlorella is a single celled green algae, it is photosynthetic although requires quite dim light for the first few weeks,and after that brighter light but not direct sunlight. The ideal temperature is 25°c (77°f), so a heating mat is highly recommended, but you can also find a warm spot without direct sunlight, and dim light for the first few weeks. One important thing to remember about chlorella, is that it will sink to the bottom of your culture jar, so make sure you give it a shake/invert it a few times to make sure it stays within the water column.
What you will need:
Chlorella starter culture
Tap water
Tap water conditioner
Cultire jar
Measuring jug
Small measure (for tap water conditioner/growth nutrient)
Optional but recommended:
Growth nutrient
Starting the culture:
I washed my hands and put gloves on so not to contaminate the culture,I have an algae culturing manual from algae research and supply in the USA, it explains about different algae and their key features.
I am using tap water, I don't want to use standard tap water due to risk of contaminants so am going to use tap water conditioner, I have a jug of 500ml of tap water, I use aquacare or API tap water conditioner. Due to them being so concentrated I am making up a much larger amount than I will actually need as it makes measuring simpler. I tend to make up 1 litre and then save it. I am using aquacare today it is 1 capful (5ml per 20litres) so I only need a tiny amount. I am using 0.01 for 500ml. I ensured there were no air bubbles and then squeezed this into the water, using the syringe to mix it in.
So here are the culture vials, I have two of these. I have a culture flask, I am starting off small and will be upgrading to larger containers as I go along. I unscrew the lid and tap the algae vials into the culture container, although not done in the video I advice using a pipette for this. I am now topping up the culture flask with the treated tap water, now there is just one last thing to add which is the growth nutrient, that is kept in my fridge.
This is my phytoplankton nutrient from ReefPhyto where I get most of my algae culturing supplies from, 3ml per 2 litres of water, I will be adding 1ml, 6ml can be added for your first 2 litres as the amount can be doubled, so 1ml should be fine, a little extra shouldn't matter too much. This nutrient is based on the Gulliard f2 medium,with a fresh syringe i am drawing up 1ml and putting this straight into the culture flask. This will now go back in my fridge. I am just shaking the flask a little to help with the aeration. So it is photosynthetic but requires dim light for the first few weeks,so I am going to put it in my bedroom where there is some light but it is mostly dim. So that is how to set up a basis chlorella culture. It does need air so I aerate by shaking the culture flask.
I hope this helps, let me know if you get stuck :)
Another great video Victoria x
Thank you x
So awesome! 💚
Thank you 💙
Great video Victoria
Thank you