Tissue Culture and Systemic Pathogens - Dr. Punja and Emerald Genomics
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- Опубликовано: 18 окт 2024
- Main focus is on SYSTEMIC PATHOGENS -what they are, what they do, how they affect yield and how can hinder resistance to other pathogens like powdery mildew, botrytis etc. Procedures to maintain a systemically clean facility.
www.sfu.ca/biol...
/ emeraldgenomics
Please give me a head's up before the next viroid/fusarium discussion. Microclone pioneered the "vigor breeding" tip cloning techniques almost a decade ago to tissue clone vigorous tips to outgrow our own Fusarium issue. It just happens to work so well for viroids as well. Love it. More, please.
I've watched your video's many times. The techniques you pioneered is really great. Easy and simple to use for a dummy like me. I would have loved to buy your kit but I live in South Africa, the freight costs are just day light robbery.
Hey Bill, how you doing
Amazing video, listened to this twice while trimming 🎉
How do you deal with fusarium . Is that something that stays in the ground every year once you have it. Can you get rid of it? I have new seeds for this year. But that plot of land was my best area
It can last 6 plus years in soil and commercial ag folks generally rotate crop or fallow field for several years. Fortunately in ground planting going to have a lot of natural competition. I’d test your soil and figure out what strain of fusarium and CFU count to see how much potential for problems. I think the non pathogenic fusarium inoculation in field will be good tool too, much better than any beneficial and potential to outcompete the pathogenic strain. Outdoors in hot and dry environment will also help plant fight infection naturally. It’s generally when rain, fog, cold, or salt stress happens that the bad symptoms can appear (weak branching, stems, stretchy plant, carrot like snap of stems, very hollow stems, lack of roots near crown of plant, slower root growth and sometimes crown rot)
Hi I am a hemp farmer in florida I Had fucereal last year It was devastating. Couldn't figure out why all my plants kept wilting and dying. . It took me the whole season to realize it was that. My tomatoes plants die the same way Once plants get sick Termite's Ate all the white stuff in the middle of the plant. My question is how long would that last in ground
Fusarium , around 3 years , don’t grow in the ground is my advice
what strand were you growing?
Interesting. That is much more rare situation. In fact I haven’t seen total loss situations except in publications and photos. That strain of fusarium you had was probably a F proliferatum- the worst most aggressive strain we’ve isolated yet.
If you send me your soil I’d be happy to culture it and find out what species.
are the spores negatively charged? could you catch them with a positive field..?
Interesting conversation. I would like to contact Brian Zimmerman , I have three strains that show a resistance to HLVd . I do test my mother stock for HLVd and fusarium.
Thanks for the information
How do you test resistance
Expose the plants to the virus, fungus, mold and test to see if it gets it.
@@jennthomas7418controlled inoculation, same for all pathogen resistance traits.
@@jennthomas7418 good question. inoculating clean lab tested plants with the disease in sterile lab and in field and observing symptoms. Run quantitative PCR to see presence and how concentrated diseases are in the plant’s tissue. Resistant could have undetectable to low amount of disease present. Could also be a strain that has high presence of disease but no significant symptoms showing.
Expose the plant to the virus and if it tests clean it has resistance.
Powdery mildew is systemic. I've done my own research and tests for the past 20 years.
I’m fairly sure fungus gnats transmit hlv and fusarium
I’m not sure about the fungus gnats but for sure root aphids will.
Fungus gnats are almost impossible to get rid of if you are using any organic material that composts. I have found a solution to them though.
Try🎉 letting top surface layer dry out between watering@max420thc
I agree. I have seen several tissue culture labs that root and or harden ex-vitro and had fungus gnats. All were positive with high levels of fusarium and or pythium. If soil particulate, air, water and touch can transmit it I think be possible for any insect as well.
@@max420thc what do you use? We have had best success with steam pasteurization to start 100% clean but only works with coco and makes organic soils and peat hydrophobic. Having our mother and veg room on positive pressure through HEPA filter has helped a lot with bugs and fungus. Heard Agratech has installed jet fans for positive filtered air for cooling greenhouses. That Beauveria bassiana strain seems to work well and not phytotoxic. Top dressing with it lasted longest and made them vanish but expensive at scale. Luckily easy to culture it on PDA and make your own home made inoculations.
Always looking for new products and ideas for fungus gnats, thrips, and aphids. Pesky bastards!
I simply grow in straight perlite and vermiculite, the critters do not like the perlite at all. This stops most of the bugs. I also use a product called t drops , and also 6 percent pyrethrins mixed at 3 ml a gallon . I have completely moved away from coco, peat, dirt. 10 years ago everyone used to sterilize their material but OMRI made them stop and we have had nothing but problems since. For spider mites I have had good success with a mixture of neem oil at one ounce a gallon, a table spoon of dawn dish soap and isopropyl alcohol at 3/4 ounce a gallon then dip the plants in it. Mitey wash dip when clones go into the cloner and coming out of the cloner.
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Does tissue culture work with Autos
Yes but only through organogenesis- multiplying callus cell masses then initiate roots and shoots when ready to grow. Some strains do really well kept in callus stage and require less maintenance than keeping them as nodal plants in media.