thanks for the video, do the same procedure every day, I try to purify a antimicrobial peptide using a polyhistidine tag and CPD (peptide-CPD-H6), the purification of the protein is optimal but when I try to cut the peptide within the column, the peptide does not appear in the wash, in fact but never leaves out the CPD-H6 with wash buffer (that's right, wash buffer), could give me some suggestions or email to communicate better? thank you very much. pd. sorry for my English, I'm from Colombia :)
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@Miiilen We don't where lab coats in our lab, only if we are working with acids, dyes, etc.
When you are sonicating anyway, what's the purpose of using Dounce homogeniser?
What do you call the glass tube and piston you use to resuspend your cell pellet?
It's called a Dounce homogenizer or a glass cell homogenizer.
thanks for the video, do the same procedure every day, I try to purify a antimicrobial peptide using a polyhistidine tag and CPD (peptide-CPD-H6), the purification of the protein is optimal but when I try to cut the peptide within the column, the peptide does not appear in the wash, in fact but never leaves out the CPD-H6 with wash buffer (that's right, wash buffer), could give me some suggestions or email to communicate better? thank you very much. pd. sorry for my English, I'm from Colombia :)
good video, helped me. Was just wondering where are the lab coats? :)
In research labs they generally don't wear labcoats Im one of the few who does
eh come on. Its an R&D lab. not a GMP facility. As long as they wear gloves its okay.
Exactly..my question..you should be in labcoats.
Eye protection??? Would love to share this, but not with lax safety standards. Hard to see lab students wearing ear buds. And no lab coats? Poor form.
Eye protection is kinda important, literally everyone wears ear buds in the lab, and lab coats are totally unimportant.
@@vintagescorpio49 agree
Unprofessional