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Hi Professor Janet. My name is Micah.

What is the best way to extract my images from H5 files? I am a new user trying to learn

Employees not wearing lab coats are unnecessarily exposing themselves to acid burns, carcinogens, mycoplasma and other microbes, and this institute is exposing itself to lawsuits, which means either there is no occupational health and safety team, or they are not doing their job to protect the institute from lawsuits.

NLT gang

This was bloody crap! you should be refluxed is the shyt music for a period of 12 hrs.

Unprofessional

Thanks for this, very helpful

Awesome

What was the movement that created this? Translational, rotational, or what?

When you are sonicating anyway, what's the purpose of using Dounce homogeniser?

eh come on. Its an R&D lab. not a GMP facility. As long as they wear gloves its okay.

One drop per well? That's it?

Eye protection??? Would love to share this, but not with lax safety standards. Hard to see lab students wearing ear buds. And no lab coats? Poor form.

Great firm and even greater work. Our project was not only completed on time but with additional advice as to our project directions. We will be happy to continue working with this firm in future.goo.gl/CVUr8a

thanks for the video, do the same procedure every day, I try to purify a antimicrobial peptide using a polyhistidine tag and CPD (peptide-CPD-H6), the purification of the protein is optimal but when I try to cut the peptide within the column, the peptide does not appear in the wash, in fact but never leaves out the CPD-H6 with wash buffer (that's right, wash buffer), could give me some suggestions or email to communicate better? thank you very much. pd. sorry for my English, I'm from Colombia :)

In research labs they generally don't wear labcoats Im one of the few who does

It's called a Dounce homogenizer or a glass cell homogenizer.

Nice video Todd

What do you call the glass tube and piston you use to resuspend your cell pellet?

guys its so cool....and science is sexy but....it seems too dangerous so why you are not wearing a coat or a goggles :)

I'm going to be freezing crystals for the first time today. Thanks for such a great video overview!

Nice development of ice rings too :)

Use a program like adxv to find your best frames. After you have identified your best frames, use only those frames in imosflm or XDS for indexing. With weak data, you may have to adjust your settings for finding and accepting reflections during indexing. There are also some more tips in the CCP4 and XDS wiki. Good luck!

Hi, do you have any tips for if the autoindexing doesn't work? I've tried using lots of different images but I have weak spots and neither mosflm nor XDS seem to be able to index my data. Suggestions would be appreciated

A really useful visual tutorial for iMosflm,thanks for uploading it!

It would be very helpful for me and people in the same stage of their jump into this field to have a more illustrated tutorial like yours. Could you please share with me some quick guides... ( I know it cant be quick but I really hate to read mathematics around it...) to master the methods.

where can I find more of this tutorial. Too impressive.. the magic was I am getting more interested to know by the videos and the coming too books (which I hated before).. Hello..Madam, I am just a beginner in structural biology... I know nothing (before today). Was feeling quiet lonely but I am trying my level best to learn this field and getting more interested as God has mercy with crystals showing up.

Great video, thanks!

@Miiilen We don't where lab coats in our lab, only if we are working with acids, dyes, etc.

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Exactly..my question..you should be in labcoats.

very useful work.... we appreciate JanetSmithLab...... keeep this work ........

APS Rocks....BIO-CAT User...LOMs would be great to show too!! Kudos to APS!!

good video, helped me. Was just wondering where are the lab coats? :)