Primary Culture of Chicken Embryos - Step 2: Embryo Extraction
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- Опубликовано: 8 фев 2022
- Video showing the removal, cleaning and termination of a chicken embryo for creation of primary culture. This is the second video in a series I will be releasing over the next few weeks. We will go from an egg to heart cells, isolated and growing on their own.
Drew, you are the best! I was a student of yours and I want to thank you so much for your amazing lectures and labs. They have made me so detail oriented that everyone at my workplace appreciates me for. I can never forget the pre labs for primary culture
That was helpful
Sir i want to perform some experiments on a muscle .... So i took out muscle from a chicken (not from embroy like after killing it) and i kept it in normal saline as a physiological solution and after adding a pinch of glucose ...will this setup of my will work and if it does how can i make muscles contract in vitro
You would have first of all egg punching with the help of egg punching machine then with the help of forcep first remove the punched egg shell,then remove the membrane and then with the help of thin pointed forcep remove the embryo it will be easily removed. Then put it in the petridish its head will be separated.
Why you do that
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The video was very well appreciated, Prof. Collop! Thank you for the clarity of your instructions :) - a dev bio student from Aberdeen, Scotland
I am currently doing a master's degree in the animal breeding department and I am also an agricultural engineer/zootechnician. And I am about to complete my master's degree by receiving a significant project scholarship in my country, which aims to manipulate the gender of chicks by injecting herbal and artificial materials with the in ovo injection method. I have provided long-term support to many projects using feed and animal materials on poultry. I am the first generation in my family to pursue higher education and I am looking for a PhD education with a scholarship. Due to my increasing interest in laboratory studies, I have also completed the laboratory veterinary health department and am in the internship phase. I am looking for an internship in a laboratory in the field of poultry nutrition or breeding for the summer term. I have a laboratory animals certificate and a B1 level English certificate. I followed some of your works on RUclips with interest and reached you. I live in Turkey and I do not want to leave the field in which I work with interest due to financial difficulties. I would be grateful if you could guide me. Best regards