This is a great introduction of the principle of confocal microscope. However, there is a mistake from 10:00 when you calculate the pinhole diameter using point spread function: D= 1.22λ/NA - this is the size of the illumination point on your sample, not the physical pinhole diameter. For physical diameter of the pinhole, it need to be multiplied by magnification (of the objective), which give the optimal pinhole diameter D = M x (1.22λ/NA), where M is the magnification of the objective lens you use with your confocal (x60, x100,etc). It is worth to point this out in case mislead people who are studying or developing system related to confocal microscopy. Overall, great video, pretty clear and straightforward explanation. Thanks for sharing:)
Thanks for pointing that out! - I was already wondering how such super-small pinholes with diameters of 500 nm would be realized mechanically.. Otherwise thanks for the great video arpan! :)
i dont completely understand the comment is sarcastic or genuine. But anyway if you find the content useful please share among your friends and help me to reach big audience
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Eugene Moon but you should have watched the advanced version of this video not this one ....it too much basics .Please watch the following ruclips.net/video/FJbn_eXzA4o/видео.html
I love the background sound “Boah”
Ha ha ha....... that sounds funny
this sound improves my focus on confocal microscopy
This was great information. Thanks for taking time to explain how Confocal Microscopy works!
Watch this one ruclips.net/video/FJbn_eXzA4o/видео.html
Please share among friends and help me to reach big audience
Excellent! Clear and concise. Many thanks.
There is a second part to it .... watch my microscopy playlist
Please share among your friends
This was an excellent explanation of this technology. Well done. Thank you very much!
Please rever to my another video for better explanation ruclips.net/video/FJbn_eXzA4o/видео.html
If like my explanation please share among friends and help me to reach big audience
This is beautifully explained, thank you so much. Phenomenal video
Please share my channel link with your friends and help me to reach big audience
A good explanation for a quick first introduction. A minor inaccuracy on the energy level diagram. Thanks and continue, please.
Thanks for your feedback and suggestions. Here is an updated version of the same video
ruclips.net/video/FJbn_eXzA4o/видео.html
Very useful. Could easily understand for the very first time. Thanks a lot. :)
thanks, more videos would be uploaded soon on confocal microscopy and image analysis
Very informative, and well said. Thank you!
Thank you, you made it easy to understand
Please share my channel link in your college group
This is a great introduction of the principle of confocal microscope. However, there is a mistake from 10:00 when you calculate the pinhole diameter using point spread function: D= 1.22λ/NA - this is the size of the illumination point on your sample, not the physical pinhole diameter. For physical diameter of the pinhole, it need to be multiplied by magnification (of the objective), which give the optimal pinhole diameter D = M x (1.22λ/NA), where M is the magnification of the objective lens you use with your confocal (x60, x100,etc). It is worth to point this out in case mislead people who are studying or developing system related to confocal microscopy. Overall, great video, pretty clear and straightforward explanation. Thanks for sharing:)
Thanks for giving a detailed feedback.
Thanks for pointing that out! - I was already wondering how such super-small pinholes with diameters of 500 nm would be realized mechanically..
Otherwise thanks for the great video arpan! :)
Very well explained
saved me from spending one hour on a book chapter.
agreed! >
U my friend is a treasure which must be save
Ok I see
i dont completely understand the comment is sarcastic or genuine. But anyway if you find the content useful please share among your friends and help me to reach big audience
Dude has mad drawing skills
Please share my channel link with your friends and juniors
Beautifully explained. Can you upload a video explaining Phase contrast microscopy? Please....
Very good! thank you!!
Please share my channel link with your friends and help me to reach big audience
This helped me a lot..thnks
There is a detailed video on same topic and I suggest you watch it
Please share among your friends and help me to reach big audience
Set for my presentation thank you
Do watch another video on confocal from my microscopy playlist....it’s a detailed one...share among your friends and help me to reach big audience
Feel free to get in touch if you need help
Awesome explanation, thanks!
Glad to know it’s helpful.... share among friends....
ruclips.net/video/FJbn_eXzA4o/видео.html in case you want details
Literally a blessing
Watch this part 2 of that video as well ruclips.net/video/FJbn_eXzA4o/видео.html
Share among friends and help them as well
Thank you so much sir
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@@animatedbiologywitharpan ofcourse sir
It helped a lot thank you so much
There is another video in the playlist named confocal microscopy details......do watch that as well
Might be helpful
Well explained
Soon I would upload a detailed video on confocal
ruclips.net/video/FJbn_eXzA4o/видео.html
If an electron goes from S0 to S1 and then back shouldn't it emmit the same light as it absorbs? What happens to the 21nm worth of light/energy?
There is an error there, there should be other vibrational states higher than S1 and the blue arrow should land there, hence the Energy difference. 😅
The formula should be D= 1.22 lambda/ 2 X NA, right?
Thank you so much
There is an advance version of this
Check my playlist...might be useful
a normal achromat is confocal if you use a pinhole
thank you
Well said
Thanks
Watch the second video about confocal for details
if both my professor and you drown in water i shall save you first
Eugene Moon please share among your friends
Eugene Moon but you should have watched the advanced version of this video not this one ....it too much basics .Please watch the following ruclips.net/video/FJbn_eXzA4o/видео.html
@@animatedbiologywitharpan i needed to learn from the crude basics, but thank you anyways :) i will watch your recommendation too thanx!!
Please use ur own accent..U already have a good one..Otherwise good video..Thanks
sakshi sood I appreciate your feedback.... it would help me to improve in future
Does anybody know the max resolution and magnification for this type of microscope?
Theoretically it can separate 2 points that are 200nm apart
Practically with higher na objective it and if you use 488 lazer to illuminate do it would be roughly 300 nm
Thank you!
confocal must not be fluorescence
"Microscopy"
Reduce back ground disturbance.
Not clear
ruclips.net/video/FJbn_eXzA4o/видео.html
Please watch this video...it would be clear
So irritating background noise.....aaahhhhhhhhhh
There is another video which don't have this back ground noise called " confocal microscopy details "
Thanks... tomorrow is IIT jam exam....
All the best
Thank u so much..