Nice explanation. Can you explain what the colors symbolize? Is black the viral particles, and what about green, red, and blue? I have a test that includes this question, please answer me
Is it in the ultracentrifugation diagram? The colours don't represent anything particular however the viral particles are obtained last whereas the cell and other debris is removed first. Hope this helps?
Thank you very much for explaining so very detailed. Is there a Study of a coronavirus where they used one of the methods you descibed in this video for purification? Also is Isolation and purification the same in Virology?
Isolation and purification are used interchangeably for a process where you obtain virus in a 'pure' form, free of cell extracts. Isolation is also used to signify cultivation of virus on a suitable medium.
There are several studies where they mention the exact method used for purification. Here I'm attaching one such research paper. Hope that helps! www.ncbi.nlm.nih.gov/pmc/articles/PMC9505060/
@@microbiologyDV hi, looking through the study you provided, there’s a lot of scientific language that flies over my head but am I correct in saying that “monkey kidney cells were used and added with P2 of SARS-CoV2 isolate Finland/1/2020 (Genbank accession number MT020781.2) “ and from that they isolated and purified their sample of SARS-CoV-2? Also, is their a study demonstrating isolation and purification of SARS-CoV-2 from a sample of a person infected with COVID-19?
Thanks a lot mam😇...mam while doing density gradient centrifugation...both types i.e isopycnic centrifugation and rate zonal centrifugation are involved at a time or these are two different types which can perform seperately?
Thank you, this was immensely helpful and well explained!! Do you know if these different methods produce equally pure viral stocks, or are some methods better at removing cellular protein/debris than others?
I have mentioned it towards the end of ultrafiltration segment. Normally a pore size of 0.002-0.1 μ and MWCO of approximately 10,000 to 100,000 Da works.
Hi ma'am we are investment firm from pune. Our board members are willing to start biotechnology company we can invest handsome amount in firm ma'am if you are able to guide us we can make you board member
I don't have notes on this topic. However you can refer Microbiology by Prescott for an overview of this topic. You can also refer the links provided in the description for details of each technique.
Thank you wonderful information
Nice explanation. Can you explain what the colors symbolize? Is black the viral particles, and what about green, red, and blue? I have a test that includes this question, please answer me
Is it in the ultracentrifugation diagram? The colours don't represent anything particular however the viral particles are obtained last whereas the cell and other debris is removed first.
Hope this helps?
Thank you very much for explaining so very detailed. Is there a Study of a coronavirus where they used one of the methods you descibed in this video for purification? Also is Isolation and purification the same in Virology?
Isolation and purification are used interchangeably for a process where you obtain virus in a 'pure' form, free of cell extracts.
Isolation is also used to signify cultivation of virus on a suitable medium.
There are several studies where they mention the exact method used for purification. Here I'm attaching one such research paper. Hope that helps!
www.ncbi.nlm.nih.gov/pmc/articles/PMC9505060/
thank you very much!@@microbiologyDV
@@microbiologyDV hi, looking through the study you provided, there’s a lot of scientific language that flies over my head but am I correct in saying that “monkey kidney cells were used and added with P2 of SARS-CoV2 isolate Finland/1/2020 (Genbank accession number MT020781.2) “ and from that they isolated and purified their sample of SARS-CoV-2?
Also, is their a study demonstrating isolation and purification of SARS-CoV-2 from a sample of a person infected with COVID-19?
Thanks a lot mam😇...mam while doing density gradient centrifugation...both types i.e isopycnic centrifugation and rate zonal centrifugation are involved at a time or these are two different types which can perform seperately?
They are used separately as the criteria for separation is contrasting in both these techniques.
@@microbiologyDV thank you mam...
Thank you, this was immensely helpful and well explained!! Do you know if these different methods produce equally pure viral stocks, or are some methods better at removing cellular protein/debris than others?
These methods have varied purity outputs so usually two or more of these methods are used in combination.
Mam aap lecture me thoda thoda hindi me bhi bata deti to bahut help ho jati mam aapka daigram stu se samjhana bahut ach ha hI
Theek hai.. Isi video with English terms ko hindi mein explain karoon toh useful hoga?
what is extremely small doesnt it have a pore size ?
I have mentioned it towards the end of ultrafiltration segment. Normally a pore size of 0.002-0.1 μ and MWCO of approximately 10,000 to 100,000 Da works.
This is the same process that I use to separate bologna.
MSc base pe
Hi ma'am we are investment firm from pune. Our board members are willing to start biotechnology company we can invest handsome amount in firm ma'am if you are able to guide us we can make you board member
Ma'am how to consult with you i need some help
You can mail me at deepthivarier@hotmail.com
Would be a great video if she didn't talk so fast. I gather English is not her first language.
Meam iske notes mil sakte hai
I don't have notes on this topic. However you can refer Microbiology by Prescott for an overview of this topic. You can also refer the links provided in the description for details of each technique.
Some fast speaking