I have a question: What if we destain too much and the bands are too faint to be visualized, can we restain the gel with Coomassie and then destain again?
Thanks! You take the ratio because it allows you to kinda normalize for background and make the standard curve linear: www.ncbi.nlm.nih.gov/pmc/articles/PMC3164080/
Very informative and I like the energy you share in the video!
Thank you!
I love you for making this video. you're a true angel, please keep making these useful videos! thank you, thank you, thank youuuuu!!!!
Glad you found it helpful!
Thank you for sharing a wonderful information
Bravo. Super helpful. Thank you so much for making this video :) Thumbs UP :)
Glad to hear it - thanks!
very clear and correct explaination. thanks alot
Thanks! Glad to hear it
I have a question: What if we destain too much and the bands are too faint to be visualized, can we restain the gel with Coomassie and then destain again?
Yes
This was super helpful!! Can you tell why we take the ratio of absorbance 590/450 while performing Bradford assay?
Thanks! You take the ratio because it allows you to kinda normalize for background and make the standard curve linear: www.ncbi.nlm.nih.gov/pmc/articles/PMC3164080/
Great explanation and so complete, thanks :) I have a question: How much time do you suggest to stain with R-250?
Thanks! Probably like 20 min or so - longer if you need more sensitivity but then the destain takes longer too! Microwaving can help
can you share the volume of staining solution used and the time used at the microwave? thanks!
Just enough to cover the gel and 30s-1 min spurts usually
@@thebumblingbiochemist thank you!
thank you very much!
You're very welcome!
LMAO I love this intro. HAHAHA
very informative, thank u
Glad it was helpful!